The Two Nucleotide-binding Domains of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Have Distinct Functions in Controlling Channel Activity

Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches

1981 • 18,584 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

G PROTEINS: TRANSDUCERS OF RECEPTOR-GENERATED SIGNALS

1987 • 6,374 citations

The P-loop — a common motif in ATP- and GTP-binding proteins

1990 • 2,029 citations

Defective intracellular transport and processing of CFTR is the molecular basis of most cystic fibrosis

1990 • 1,756 citations

Structural model of ATP-binding proteing associated with cystic fibrosis, multidrug resistance and bacterial transport

1990 • 1,149 citations

Fast events in single‐channel currents activated by acetylcholine and its analogues at the frog muscle end‐plate.

1985 • 768 citations

Generation of cAMP-Activated Chloride Currents by Expression of CFTR

1991 • 550 citations

Nucleoside triphosphates are required to open the CFTR chloride channel

1991 • 520 citations

Use of a hybrid vaccinia virus-T7 RNA polymerase system for expression of target genes.

1987 • 409 citations

Substrate positions and induced-fit in crystalline adenylate kinase

1977 • 304 citations

Discrete mutations introduced in the predicted nucleotide-binding sites of the mdr1 gene abolish its ability to confer multidrug resistance.

1989 • 303 citations

Expression of Gsα in Escherichia coli

1989 • 262 citations

Regulation of the gating of cystic fibrosis transmembrane conductance regulator C1 channels by phosphorylation and ATP hydrolysis.

1994 • 262 citations

The influence of bound GDP on the kinetics of guanine nucleotide binding to G proteins.

1986 • 261 citations

Regulation by ATP and ADP of CFTR Chloride Channels That Contain Mutant Nucleotide-Binding Domains

1992 • 231 citations

Cystic fibrosis transmembrane conductance regulator: A chloride channel with novel regulation

1992 • 214 citations

Coupling of CFTR Cl− channel gating to an ATP hydrolysis cycle

1994 • 212 citations

Statistical methods for model discrimination. Applications to gating kinetics and permeation of the acetylcholine receptor channel

1987 • 199 citations

Structural model of the nucleotide-binding conserved component of periplasmic permeases.

1991 • 183 citations

Structure-function analysis of the histidine permease and comparison with cystic fibrosis mutations.

1991 • 173 citations

The protein kinase A-regulated cardiac CI− channel resembles the cystic fibrosis transmembrane conductance regulator

1992 • 170 citations

Control of CFTR chloride conductance by ATP levels through non-hydrolytic binding

1992 • 167 citations

Regulation of the cystic fibrosis transmembrane conductance regulator Cl- channel by negative charge in the R domain.

1993 • 148 citations

Effect of ATP concentration on CFTR Cl- channels: a kinetic analysis of channel regulation

1994 • 122 citations

Stretch Activation of a K+ Channel in Molluscan Heart Cells

1987 • 117 citations

The amino-terminal portion of CFTR forms a regulated CI− channel

1994 • 113 citations

Cystic Fibrosis Transmembrane Conductance Regulator: Nucleotide Binding to a Synthetic Peptide

1991 • 100 citations

Functional roles of the nucleotide-binding folds in the activation of the cystic fibrosis transmembrane conductance regulator.

1993 • 92 citations

Recombinant synthesis, purification, and nucleotide binding characteristics of the first nucleotide binding domain of the cystic fibrosis gene product.

1992 • 72 citations

Interaction of nucleotides with membrane-associated cystic fibrosis transmembrane conductance regulator

1993 • 61 citations

Mechanism of adenylate kinase. 6. Are the essential lysines essential?

1990 • 60 citations

Solution structure of the 45-residue magnesium ATP-binding peptide of adenylate kinase as examined by 2-D NMR, FTIR, and CD spectroscopy

1988 • 59 citations

Effect of deletion mutations on the function of CFTR chloride channels.

1993 • 51 citations

Regulation of CFTR Cl- conductance in secretion by cellular energy levels

1993 • 42 citations

The cystic fibrosis transmembrane conductance regulator. Nucleotide binding to a synthetic peptide segment from the second predicted nucleotide binding fold.

1994 • 26 citations

Partial purification of the cystic fibrosis transmembrane conductance regulator.

1992 • 20 citations