Disruption of an ionic network leads to accelerated thermal denaturation of d-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic bacterium Thermotoga maritima

MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures

1991 • 14,222 citations

The Activated Complex in Chemical Reactions

1935 • 5,261 citations

Helix stabilization by Glu-...Lys+ salt bridges in short peptides of de novo design.

1987 • 930 citations

Thermotoga maritima sp. nov. represents a new genus of unique extremely thermophilic eubacteria growing up to 90�C

1986 • 762 citations

A general method for rapid site-directed mutagenesis using the polymerase chain reaction

1990 • 642 citations

Do salt bridges stabilize proteins? A continuum electrostatic analysis

1994 • 629 citations

Protein stability and molecular adaptation to extreme conditons

1991 • 609 citations

STRUCTURAL AND GENETIC ANALYSIS OF PROTEIN STABILITY

1993 • 455 citations

Stereochemical basis of heat stability in bacterial ferredoxins and in haemoglobin A2

1975 • 420 citations

Strength and co-operativity of contributions of surface salt bridges to protein stability

1990 • 415 citations

The structure of Pyrococcus furiosus glutamate dehydrogenase reveals a key role for ion-pair networks in maintaining enzyme stability at extreme temperatures

1995 • 409 citations

Thermozymes: Identifying molecular determinants of protein structural and functional stability

1996 • 292 citations

Side‐chain conformational entropy in protein folding

1995 • 285 citations

ENZYMES UNDER EXTREMES OF PHYSICAL CONDITIONS

1981 • 275 citations

The energetics of ion-pair and hydrogen-bonding interactions in a helical peptide

1993 • 260 citations

Contributions of engineered surface salt bridges to the stability of T4 lysozyme determined by directed mutagenesis

1991 • 238 citations

2.0 å structure of indole-3-glycerol phosphate synthase from the hyperthermophile Sulfolobus solfataricus: possible determinants of protein stability

1995 • 224 citations

Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR)

1989 • 218 citations

The Crystal Structure of Holo-glyceraldehyde-3-phosphate Dehydrogenase from the Hyperthermophilic BacteriumThermotoga maritimaat 2.5 Å Resolution

1995 • 218 citations

Extremely thermostable D-glyceraldehyde-3-phosphate dehydrogenase from the eubacterium Thermotoga maritima

1990 • 211 citations

Determinants of Enzyme Thermostability Observed in the Molecular Structure ofThermusaquaticus<scp>d</scp>-Glyceraldehyde-3-phosphate Dehydrogenase at 2.5 Å Resolution^,

1996 • 204 citations

X‐ray crystal structures of the oxidized and reduced forms of the rubredoxin from the marine hyperthermophilic archaebacterium pyrococcus furiosus

1992 • 190 citations

Surface electrostatic interactions contribute little to stability of barnase

1991 • 168 citations

Structure and Stability of Hyperstable Proteins: Glycolytic Enzymes From Hyperthermophilic Bacterium Thermotoga Maritima

1996 • 156 citations

Role of bound calcium ions in thermostable, proteolytic enzymes. Separation of intrinsic and calcium ion contributions to the kinetic thermal stability

1976 • 146 citations

NMR Structure of HMfB from the Hyperthermophile,Methanothermus fervidus, Confirms that this Archaeal Protein is a Histone

1996 • 144 citations

Crystal structures of Escherichia coli and Salmonella typhimurium 3-isopropylmalate dehydrogenase and comparison with their thermophilic counterpart from Thermus thermophilus

1997 • 140 citations

Determinants of protein thermostability observed in the 1.9-.ANG. crystal structure of malate dehydrogenase from the thermophilic bacterium Thermus flavus

1993 • 138 citations

Crystal structure of thermitase at 1.4A˚resolution

1990 • 137 citations

KGB: a single buffer for all restriction endonucleases

1988 • 130 citations

Temperature adaptation of lactate dehydrogenase Structural, functional and genetic aspects

1988 • 120 citations

Crystal Structure of Ribonuclease H from Thermus thermophilus HB8 Refined at 2·8 Å Resolution

1993 • 119 citations

Heat Stability of a Tetrameric Enzyme, D-Glyceraldehyde-3-PhosphateDehydrogenase

1980 • 119 citations

Contributions of a hydrogen bond/salt bridge network to the stability of secondary and tertiary structure in λ repressor

1994 • 117 citations

Closed structure of phosphoglycerate kinase from Thermotoga maritima reveals the catalytic mechanism and determinants of thermal stability

1997 • 113 citations

Stability and folding of ultrastable proteins: eye lens crystalline and enzymes from thermophiles ¹

1996 • 108 citations

Comparison of the Structures of Wild-type and a N313T Mutant ofEscherichia coliGlyceraldehyde 3-Phosphate Dehydrogenases: Implication for NAD Binding and Cooperativity

1996 • 94 citations

Electrostatic effects on protein stability

1992 • 73 citations

How to make my blood boil

1995 • 73 citations

Stability and reconstitution of D-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic eubacterium Thermotoga maritima.

1992 • 71 citations

Engineering enzymes for stability

1996 • 62 citations

Role of Calcium Ions in the Thermostability of Thermolysin and Bacillus subtilis var. amylosacchariticus Neutral Protease

1976 • 60 citations

An improved thermal cycle for two-step PCR-based targeted mutagenesis

1992 • 59 citations

Hyperthermophiles: taking the heat and loving it

1995 • 58 citations

D-Glyceraldehyde-3-Phosphate Dehydrogenase. The Purification and Characterisation of the Enzyme from the Thermophiles Bacillus stearothermophilus and Thermus aquaticus

1980 • 46 citations

Sequence determinants of folding and stability for the P22 Arc repressor dimer

1996 • 45 citations

The effect of ion pairs on the thermal stability of D-glyceraldehyde 3-phosphate dehydrogenase from the hyperthermophilic bacterium Thermotoga maritima

1994 • 41 citations

Functional expression of <scp>d</scp>‐glyceraldehyde‐3‐phosphate dehydrogenase from the hyperthermophilic eubacterium Thermotoga maritima in Escherichia coli

1993 • 36 citations

Electrostatic effects on protein stability

1992 • 30 citations

Structural features responsible for kinetic thermal stability of a carboxypeptidase from the archaebacterium Sulfolobus solfataricus

1993 • 25 citations

Extremely thermostable L(+)‐lactate dehydrogenase from thermotoga maritima: Cloning, characterization, and crystallization of the recombinant enzyme in its tetrameric and octameric state

1996 • 22 citations

The effect of Na+ and K+ on the thermal denaturation of Na+ and + K+-dependent ATPase

1983 • 17 citations