Structural and Functional Characterization of the Human Perlecan Gene Promoter

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1984 • 4,899 citations

Transcriptional Regulation in Mammalian Cells by Sequence-Specific DNA Binding Proteins

1989 • 3,219 citations

The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene

1990 • 993 citations

[36] Eukaryotic gene transcription with purified components

1983 • 992 citations

Compilation of vertebrate-encoded transcription factors

1992 • 934 citations

Heparan sulphate bound growth factors: a mechanism for stromal cell mediated haemopoiesis

1988 • 845 citations

Human CCAAT-binding proteins have heterologous subunits

1988 • 712 citations

Proteoglycans of the extracellular environment: clues from the gene and protein side offer novel perspectives in molecular diversity and function

1996 • 639 citations

A nuclear factor 1 binding site mediates the transcriptional activation of a type I collagen promoter by transforming growth factor-β

1988 • 620 citations

The Degradation of Human Endothelial Cell-derived Perlecan and Release of Bound Basic Fibroblast Growth Factor by Stromelysin, Collagenase, Plasmin, and Heparanases

1996 • 601 citations

Perlecan, basal lamina proteoglycan, promotes basic fibroblast growth factor-receptor binding, mitogenesis, and angiogenesis

1994 • 541 citations

Analysis of the DNA-binding and activation properties of the human transcription factor AP-2.

1991 • 519 citations

The complete sequence of perlecan, a basement membrane heparan sulfate proteoglycan, reveals extensive similarity with laminin A chain, low density lipoprotein-receptor, and the neural cell adhesion molecule.

1991 • 446 citations

The biology of perlecan: the multifaceted heparan sulphate proteoglycan of basement membranes and pericellular matrices

1994 • 384 citations

Primary structure of the human heparan sulfate proteoglycan from basement membrane (HSPG2/perlecan). A chimeric molecule with multiple domains homologous to the low density lipoprotein receptor, laminin, neural cell adhesion molecules, and epidermal growth factor.

1992 • 307 citations

Differential regulation of extracellular matrix proteoglycan (PG) gene expression. Transforming growth factor-beta 1 up-regulates biglycan (PGI), and versican (large fibroblast PG) but down-regulates decorin (PGII) mRNA levels in human fibroblasts in culture

1991 • 261 citations

Transcription Factors and Hematopoietic Development

1995 • 236 citations

Basement‐membrane heparan sulfate proteoglycan binds to laminin by its heparan sulfate chains and to nidogen by sites in the protein core

1992 • 193 citations

Endothelial cells interact with the core protein of basement membrane perlecan through beta 1 and beta 3 integrins: an adhesion modulated by glycosaminoglycan.

1992 • 180 citations

Widespread expression of perlecan proteoglycan in basement membranes and extracellular matrices of human tissues as detected by a novel monoclonal antibody against domain III and by in situ hybridization.

1994 • 180 citations

Proteoglycans of basement membranes

1993 • 166 citations

Characterization of the complete genomic structure of the human versican gene and functional analysis of its promoter

1994 • 154 citations

Perlecan is a component of cartilage matrix and promotes chondrocyte attachment

1995 • 151 citations

Adenovirus DNA replication in vitro: synthesis of full-length DNA with purified proteins.

1983 • 148 citations

Biosynthesis of heparan sulfate proteoglycan by human colon carcinoma cells and its localization at the cell surface.

1984 • 145 citations

Transforming-growth-factor-β activation elements in the distal promoter regions of the rat α1 type I collagen gene

1991 • 138 citations

Transcriptional Regulation of Decorin Gene Expression

1995 • 130 citations

Structural characterization of the complete human perlecan gene and its promoter.

1993 • 130 citations

Transcriptional Regulation of the Mouse αA-Crystallin Gene: Activation Dependent on a Cyclic AMP-Responsive Element (DE1/CRE) and a Pax-6-Binding Site

1995 • 127 citations

Abnormal expression of perlecan proteoglycan in metastatic melanomas.

1994 • 118 citations

Transforming Growth Factor βl-Responsive Element: Closely Associated Binding Sites for USF and CCAAT-Binding Transcription Factor-Nuclear Factor I in the Type 1 Plasminogen Activator Inhibitor Gene

1992 • 112 citations

High-affinity binding of interferon-gamma to a basement membrane complex (matrigel).

1991 • 109 citations

Regulation of the alpha 1(I) collagen promoter via a transforming growth factor-beta activation element

1993 • 109 citations

The human alpha 2 integrin gene promoter. Identification of positive and negative regulatory elements important for cell-type and developmentally restricted gene expression.

1994 • 109 citations

Nuclear factor-I and activator protein-2 bind in a mutually exclusive way to overlapping promoter sequences andtrans-activate the human growth hormone gene

1990 • 102 citations

Isolation and characterization of fibronectin-binding proteoglycan carrying both heparan sulfate and dermatan sulfate chains from human placenta.

1987 • 89 citations

Overlapping Egr-1 and Sp1 sites function in the regulation of transcription of the mouse thrombospondin 1 gene.

1994 • 87 citations

Structural and functional characterization of the human decorin gene promoter. A homopurine-homopyrimidine S1 nuclease-sensitive region is involved in transcriptional control.

1994 • 78 citations

Transforming growth factor beta alters the expression of heparan sulfate proteoglycan in human colon carcinoma cells.

1990 • 70 citations

Perlecan in human bone marrow: A growth-factor-presenting, but anti-adhesive, extracellular matrix component for hematopoietic cells

1995 • 69 citations

The Murine Biglycan: Complete cDNA Cloning, Genomic Organization, Promoter Function, and Expression

1995 • 58 citations

Heparan sulfate proteoglycan of human colon: Partial molecular cloning, cellular expression, and mapping of the gene (HSPG2) to the short arm of human chromosome 1

1991 • 56 citations

Establishment of a Cell Line from the EHS Tumor: Biosynthesis of Basement Membrane Constituents and Characterization of a Hybrid Proteoglycan Containing Heparan and Chondroitin Sulfate Chains

1992 • 50 citations

Structural basis of beta 1 integrin-mediated cell adhesion to a large heparan sulfate proteoglycan from basement membranes.

1993 • 48 citations

Identification of the precursor protein for the heparan sulfate proteoglycan of human colon carcinoma cells and its post-translational modifications

1989 • 45 citations

Identification of a TGF-β Responsive Element in the Human Elastin Promoter

1994 • 44 citations

Transforming growth factor beta 1 stimulates the production of basic fibroblast growth factor binding proteoglycans in Balb/c3T3 cells.

1992 • 43 citations

Functional Characterization of Mouse Syndecan-1 Promoter

1996 • 43 citations

Antibody mapping and tissue localization of globular and cysteine-rich regions of perlecan domain III.

1995 • 37 citations

Binding of a nuclear protein to the rat growth hormone silencer element

1992 • 36 citations

Fatty acylation of heparan sulfate proteoglycan from human colon carcinoma cells.

1990 • 30 citations

The proteoglycan perlecan is expressed in the erythroleukemia cell line K562 and is upregulated by sodium butyrate and phorbol ester

1995 • 30 citations

Phorbol esters activate proteoglycan metabolism in human colon cancer cells en route to terminal differentiation.

1990 • 18 citations

SV40 large-T oncogene inhibits transcription of perlecan-related proteoglycans but stimulates hyaluronan synthesis in a temperature-sensitive renal-tubule principal cell line

1994 • 9 citations