Quantitation of RNA using the polymerase chain reaction

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

PCR protocols — A guide to methods and applications

1990 • 12,897 citations

PCR protocols — A guide to methods and applications

1990 • 10,078 citations

Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia

1985 • 9,128 citations

High Levels of HIV-1 in Plasma During All Stages of Infection Determined By Competitive PCR

1993 • 1,263 citations

Effects of primer-template mismatches on the polymerase chain reaction: Human immunodeficiency virus type 1 model studies

1990 • 1,037 citations

Transcription of the dystrophin gene in human muscle and non-muscle tissues

1988 • 733 citations

Absolute mRNA quantification using the polymerase chain reaction (PCR). A novel approach by aPCR aidedtranscipttitration assay (PATTY)

1989 • 659 citations

Human immunodeficiency virus-infected individuals contain provirus in small numbers of peripheral mononuclear cells and at low copy numbers

1990 • 605 citations

Competitive PCR

1992 • 528 citations

Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase

1991 • 475 citations

Use of the polymerase chain reaction in the quantitation of MDR-1 gene expression

1990 • 427 citations

PCR MIMICS: competitive DNA fragments for use as internal standards in quantitative PCR.

1993 • 374 citations

Quantitative or semi-quantitative PCR: reality versus myth.

1992 • 351 citations

Transient Expression of Progesterone Receptor Messenger RNA in Ovarian Granuiosa Cells after the Preovulatory Luteinizing Hormone Surge

1991 • 335 citations

Quantitative competitive polymerase chain reaction for accurate quantitation of HIV DNA and RNA species.

1993 • 319 citations

Cellular Localization and Hormonal Regulation of Follicle-Stimulating Hormone and Luteinizing Hormone Receptor Messenger RNAs in the Rat Ovary

1991 • 299 citations

Ubiquitous MyoD transcription at the midblastula transition precedes induction-dependent MyoD expression in presumptive mesoderm of X. laevis

1991 • 248 citations

Dynamics of GATA transcription factor expression during erythroid differentiation

1993 • 238 citations

Pcr in Situ Hybridization: Protocols and Applications

1997 • 208 citations

Quantitative estimation of minor mRNAs by cDNA‐polymerase chain reaction

1990 • 196 citations

Quantitative titration of nucleic acids by enzymatic amplification reactions run to saturation

1993 • 160 citations

Cell cycle regulation of the glyceraldehyde3phosphate dehydrogenaseluracil DNA glycosylase gene in normal human cells

1993 • 157 citations

Quantitative PCR and RT-PCR in virology.

1993 • 150 citations

Individual stage selector element mutations lead to reciprocal changes in beta- vs. epsilon-globin gene transcription: genetic confirmation of promoter competition during globin gene switching.

1992 • 101 citations

Determining transcript number using the polymerase chain reaction:Pgk-2,mP2, andPGK-2transgene mRNA levels during spermatogenesis

1991 • 77 citations

Single-step elimination of contaminating DNA prior to reverse transcriptase PCR.

1992 • 66 citations

PCR in Situ Hybridization: Protocols and Applications

1993 • 59 citations

Whole Animal Cell Sorting of Drosophila Embryos

1991 • 48 citations

RNA template-specific PCR: an improved method that dramatically reduces false positives in RT-PCR.

1991 • 46 citations

cDNA Equalization for Reverse Transcription-Polymerase Chain Reaction Quantitation

1993 • 43 citations

Thermocycler temperature variation invalidates PCR results.

1990 • 40 citations

RT-PCR artifacts from processed pseudogenes.

1991 • 34 citations

Molecular mechanics of nucleic acid sequence amplification

1993 • 24 citations