Messenger RNA translation in prokaryotes: GTPase centers associated with translational factors

Escherichia Coli and Salmonella: Typhimurium Cellular and Molecular Biology

1987 • 4,385 citations

The GTPase superfamily: conserved structure and molecular mechanism

1991 • 3,365 citations

Electron Spin Resonance

1968 • 1,711 citations

Structure of the guanine-nucleotide-binding domain of the Ha-ras oncogene product p21 in the triphosphate conformation

1989 • 881 citations

Structure, Function, and Genetics of Ribosomes

1986 • 852 citations

Crystal Structure of the Ternary Complex of Phe-tRNA ^Phe , EF-Tu, and a GTP Analog

1995 • 846 citations

The 2.2 Å crystal structure of transducin-α complexed with GTPγS

1993 • 788 citations

Intermediate states in the movement of transfer RNA in the ribosome

1989 • 736 citations

STRUCTURE AND FUNCTION OF SIGNAL-TRANSDUCING GTP-BINDING PROTEINS

1991 • 630 citations

Structure of the GDP Domain of EF-Tu and Location of the Amino Acids Homologous to ras Oncogene Proteins

1985 • 626 citations

GTP-binding domain: three consensus sequence elements with distinct spacing.

1987 • 602 citations

Termination of translation in eukaryotes is governed by two interacting polypeptide chain release factors, eRF1 and eRF3.

1995 • 589 citations

The role of guanosine 5′-triphosphate in polypeptide chain elongation

1978 • 559 citations

Crystal structure of active elongation factor Tu reveals major domain rearrangements

1993 • 533 citations

The Ribosome : structure, function, and evolution

1990 • 508 citations

Initiation of mRNA translation in prokaryotes

1990 • 501 citations

Molecular Mechanisms of Protein Biosynthesis

1977 • 472 citations

Gene for a novel tRNA species that accepts L-serine and cotranslationally inserts selenocysteine

1988 • 434 citations

A highly conserved eukaryotic protein family possessing properties of polypeptide chain release factor

1994 • 407 citations

The crystal structure of elongation factor EF-Tu from Thermus aquaticus in the GTP conformation

1993 • 399 citations

Structural details of the binding of guanosine diphosphate to elongation factor Tu from E. coli as studied by X-ray crystallography.

1985 • 396 citations

The crystal structure of elongation factor G complexed with GDP, at 2.7 A resolution.

1994 • 377 citations

Release factors differing in specificity for terminator codons.

1968 • 374 citations

Three-dimensional structure of the ribosomal translocase: elongation factor G from Thermus thermophilus.

1994 • 367 citations

Binding of tRNA to the ribosomal A and P sites protects two distinct sets of nucleotides in 16 S rRNA

1990 • 294 citations

The structure of the Escherichia coli EF-Tu· EF-Ts complex at 2.5 Å resolution

1996 • 289 citations

Refined structure of elongation factor EF-Tu from Escherichia coli

1992 • 287 citations

Identification of a novel translation factor necessary for the incorporation of selenocysteine into protein

1989 • 269 citations

Features of the formate dehydrogenase mRNA necessary for decoding of the UGA codon as selenocysteine.

1990 • 264 citations

Identification of two copies of the gene for the elongation factor EF–Tu in E. Coli

1975 • 259 citations

A Model for the Tertiary Structure of p21, the Product of the ras Oncogene

1985 • 248 citations

Eukaryotic polypeptide chain release factor eRF3 is an eRF1- and ribosome-dependent guanosine triphosphatase.

1996 • 245 citations

Selection of the initiator tRNA by Escherichia coli initiation factors.

1989 • 237 citations

Ribosome gymnastics—Degree of difficulty 9.5, style 10.0

1990 • 226 citations

The nucleotide sequence of tufB and four nearby tRNA structural genes of Escherichia coli

1980 • 213 citations

GTP consumption of elongation factor Tu during translation of heteropolymeric mRNAs.

1995 • 209 citations

Selenocysteine synthase from Escherichia coli. Analysis of the reaction sequence.

1991 • 198 citations

Light-scattering studies showing the effect of initiation factors on the reversible dissociation of Escherichia coli ribosomes

1975 • 191 citations

The Translational Apparatus

1993 • 190 citations

Escherichia coli genes whose products are involved in selenium metabolism

1988 • 182 citations

Comparison of guanosine triphosphate split and polypeptide synthesis with a purified E. coli system.

1966 • 176 citations

The primary structure of the α subunit of human elongation factor 1

1986 • 173 citations

Selection of the mRNA translation initiation region by Escherichia coli ribosomes.

1988 • 170 citations

The nucleotide sequence of the cloned tufA gene of Escherichia coli

1980 • 163 citations

Structural studies of ribosomes

1982 • 159 citations

The allosteric three-site model for the ribosomal elongation cycle: features and future

1990 • 154 citations

A mutation that alters the nucleotide specificity of elongation factor Tu, a GTP regulatory protein.

1987 • 153 citations

Sequential Translation of Trinucleotide Codons for the Initiation and Termination of Protein Synthesis

1968 • 148 citations

Factors Involved in the Transfer of Aminoacyl-tRNA to the Ribosome

1977 • 142 citations

EVOLVING RIBOSOME STRUCTURE: DOMAINS IN ARCHAEBACTERIA, EUBACTERIA, EOCYTES AND EUKARYOTES

1985 • 140 citations

Translational termination: "stop" for protein synthesis or "pause" for regulation of gene expression

1992 • 139 citations

Initiator transfer RNAs

1994 • 137 citations

Conserved motifs in prokaryotic and eukaryotic polypeptide release factors: tRNA-protein mimicry hypothesis.

1996 • 136 citations

Identification of the prfC gene, which encodes peptide-chain-release factor 3 of Escherichia coli.

1994 • 134 citations

Relative affinities of all Escherichia coli aminoacyl-tRNAs for elongation factor Tu-GTP.

1984 • 131 citations

Localization and characterization of the gene encoding release factor RF3 in Escherichia coli.

1994 • 123 citations

Properties and regulation of the GTPase activities of elongation factors Tu and G, and of initiation factor 2

1981 • 120 citations

Peptide chain termination

1980 • 120 citations

Toward a Model for the Interaction Between Elongation Factor Tu and the Ribosome

1993 • 117 citations

Occurrence in vivo of selenocysteyl-tRNAUCASER in Escherichia coli

1989 • 116 citations

Ribosome recycling factor (ribosome releasing factor) is essential for bacterial growth.

1994 • 114 citations

Elongation factor Tu: a regulatory GTPase with an integrated effector

1994 • 113 citations

Ribosomal Translocation: Facts and Models

1985 • 112 citations

The length of the aminoacyl-acceptor stem of the selenocysteine-specific tRNA(Sec) of Escherichia coli is the determinant for binding to elongation factors SELB or Tu.

1991 • 112 citations

Organization and nucleotide sequence of a new ribosomal operon in Escherichia coli containing the genes for ribosomal protein S2 and elongation factor Ts

1981 • 110 citations

Sequence of the initiation factor IF2 gene: unusual protein features and homologies with elongation factors.

1984 • 107 citations

Kinetic studies of Escherichia coli elongation factor Tu-guanosine 5'-triphosphate-aminoacyl-tRNA complexes

1985 • 100 citations

Interaction of bacterial initiation factor 2 with initiator tRNA.

1976 • 98 citations

Chemical crosslinking of elongation factor G to the 23S RNA in 70S ribosomes fromEscherichia coli

1983 • 96 citations

Interaction of translation factor SELB with the formate dehydrogenase H selenopolypeptide mRNA.

1993 • 95 citations

How many EF-Tu molecules participate in aminoacyl-tRNA binding and peptide bond formation in Escherichia coli translation?

1990 • 92 citations

The nucleotide sequence of theEscherichta coli fusgene, coding for elongation factor G

1984 • 88 citations

Antideterminants present in minihelix(Sec) hinder its recognition by prokaryotic elongation factor Tu.

1996 • 85 citations

Polypeptide elongation and tRNA cycling in Escherichia coli: A dynamic approach

1980 • 85 citations

Cross-linking of initiation factor IF-2 to Escherichia coli 30 S ribosomal proteins with dimethylsuberimidate

1975 • 82 citations

Mutants of elongation factor Tu promote ribosomal frameshifting and nonsense readthrough.

1987 • 82 citations

The selenocysteine-inserting opal suppressor serine tRNA fromE.coliis highly unusual in structure and modification

1989 • 82 citations

Properties of a genetically engineered G domain of elongation factor Tu.

1987 • 81 citations

Peptide Chain Termination, Codon, Protein Factor, and Ribosomal Requirements

1969 • 81 citations

Solution Structure of Selenocysteine-inserting tRNASec from Escherichia coli

1993 • 80 citations

Interaction between Guanosine Derivatives and Factors involved in the Initiation of Protein Synthesis

1970 • 78 citations

Mutants of initiator tRNA that function both as initiators and elongators.

1991 • 77 citations

RELEASE OF TRANSFER RNA DURING PEPTIDE CHAIN ELONGATION

1969 • 76 citations

Guanosine 5'-triphosphatase activity of initiation factor f2.

1968 • 75 citations

The GTPase superfamily

1993 • 75 citations

Location of the tufB promoter of E. coil: Cotranscription of tufB with four transfer RNA genes

1981 • 72 citations

Hydrolysis of GTP by elongation factor Tu can be induced by monovalent cations in the absence of other effectors.

1982 • 71 citations

Direct crosslinks between initiation factors 1, 2, and 3 and ribosomal proteins promoted by 2-iminothiolane

1983 • 70 citations

Selenocysteine, a highly specific component of certain enzymes, is incorporated by a UGA-directed co-translational mechanism.

1988 • 70 citations

Peptide Chain Termination with Mammalian Release Factor

1970 • 67 citations

Molecular dissection of translation initiation factor IF2. Evidence for two structural and functional domains.

1991 • 66 citations

Structure-function relationships in the GTP binding domain of EF-Tu: mutation of Val20, the residue homologous to position 12 in p21.

1988 • 66 citations

Interaction of a selenocysteine-incorporating tRNA with elongation factor Tu fromE.coli

1990 • 64 citations

The Interaction of Radioactive Initiation Factor IF-2 with Ribosomes during Initiation of Protein Synthesis

1973 • 63 citations

Purification and properties of ribosome-releasing factor

1972 • 62 citations

A severely truncated form of translational initiation factor 2 supports growth of Escherichia coli

1991 • 62 citations

Peptide Chain Termination: Effect of Protein S on Ribosomal Binding of Release Factors

1970 • 62 citations

Purification and Properties of Factor G

1969 • 61 citations

Structure-function relationship in Escherichia coli initiation factors. Biochemical and biophysical characterization of the interaction between IF-2 and guanosine nucleotides.

1985 • 61 citations

Substitution of histidine-84 and the GTPase mechanism of elongation factor Tu

1991 • 60 citations

Structure of the ternary complex of EF-Tu: macromolecular mimicry in translation

1996 • 60 citations

Structural determination of the functional sites of E. coli elongation factor Tu

1990 • 57 citations

Two translational initiation sites in the infB gene are used to express initiation factor IF2 alpha and IF2 beta in Escherichia coli.

1985 • 57 citations

The protein synthesis initiation factor 2 G-domain. Study of a functionally active C-terminal 65-kilodalton fragment of IF2 from Escherichia coli

1987 • 56 citations

Cloning and mapping of a gene for translational initiation factor IF2 in Escherichia coli.

1982 • 54 citations

Relevance of histidine-84 in the elongation factor Tu GTPase activity and in poly(Phe) synthesis: Its substitution by glutamine and alanine

1995 • 53 citations

The Solution Structure of the Escherichia coli Initiator tRNA and Its Interactions with Initiation Factor 2 and the Ribosomal 30 S Subunit

1989 • 51 citations

Membrane‐associated GTPases in bacteria

1992 • 51 citations

Both forms of translational initiation factor IF2 (α and β) are required for maximal growth of Escherichia coli

1992 • 50 citations

The elongation factor Tu coded by the tufA gene of Escherichia coli K-12 is almost identical to that coded by the tufB gene.

1977 • 50 citations

The binding of fluorescein-labeled protein synthesis initiation factor 2 to Escherichia coli 30 S ribosomal subunits determined by fluorescence polarization.

1982 • 50 citations

On the Structure, Function, and Dynamics of L7/L12 from Escherichia coliRibosomes

1986 • 49 citations

Recognition of the mRNA selenocysteine insertion sequence by the specialized translational elongation factor SELB.

1994 • 48 citations

A study of the kinetic mechanism of elongation factor Ts.

1985 • 47 citations

Minimalist Aminoacylated RNAs as Efficient Substrates for Elongation Factor Tu

1994 • 46 citations

Truncated elongation factor G lacking the G domain promotes translocation of the 3' end but not of the anticodon domain of peptidyl-tRNA.

1996 • 45 citations

Macromolecular arrangement in the aminoacyl-tRNA.cntdot.elongation factor Tu.cntdot.GTP ternary complex. A fluorescence energy transfer study

1995 • 44 citations

Sequence comparison of new prokaryotic and mitochondrial members of the polypeptide chain release factor family predicts a five-domain model for release factor structure

1992 • 44 citations

Site-Directed Mutagenesis of Thermus thermophilus Elongation Factor Tu. Replacement of His85, Asp81 and Arg300

1995 • 44 citations

Mutagenesis ofselC, the gene for the selenocysteine-insertlng tRNA-species inE.coli: effects onin vivofunction

1990 • 43 citations

Mutagenesis of bacterial elongation factor Tu at lysine 136

1989 • 42 citations

Characterization of the secondary structure of calmodulin in complex with a calmodulin-binding domain peptide

1992 • 41 citations

Translational Frameshifts Induced by Mutant Species of the Polypeptide Chain Elongation Factor Tu of Escherichia coli

1989 • 41 citations

Chemical Cross‐Linking of Elongation Factor G to both Subunits of the 70‐S Ribosomes from Escherichia coli

1982 • 39 citations

Preparation and characterization of two monoclonal antibodies against different epitopes in Escherichia coli ribosomal protein L7/L12.

1985 • 38 citations

Function of Polypeptide Chain Release Factor RF-3 in Escherichia coli

1995 • 37 citations

A ribosomal frameshifting error during translation of the argl mRNA of Escherichla coli

1994 • 37 citations

Photochemical Cross‐Linking of Elongation Factor G to 70‐S Ribosomes from Escherichia coli by 4‐(6‐Formyl‐3‐azidophenoxy)butyrimidate

1981 • 36 citations

A single amino acid substitution in elongation factor Tu disrupts interaction between the ternary complex and the ribosome

1993 • 36 citations

Cross-linking of initiation factor IF2 to proteins L7/L12 in 70 S ribosomes of Escherichia coli

1976 • 36 citations

Ternary complexes of Escherichia coli aminoacyl-tRNAs with the elongation factor Tu and GTP: Thermodynamic and structural studies

1990 • 35 citations

Direct demonstration of duplicate tuf genes in enteric bacteria.

1978 • 35 citations

Nucleotide binding and GTP hydrolysis by elongation factor Tu from Thermus thermophilus as monitored by proton NMR

1992 • 34 citations

Suppression of Temperature-sensitive Defects of Polypeptide Release Factors RF-1 and RF-2 by Mutations or by an Excess of RF-3 in

1996 • 33 citations

The elongation factor G carries a catalytic site for GTP hydrolysis, which is revealed by using 2-propanol in the absence of ribosomes.

1986 • 33 citations

Peptide Chain Termination, VI. Purification and Site of Action of S

1970 • 32 citations

Read-through translation

1983 • 32 citations

The role of EF-Tu and other translation components in determining translocation step size

1990 • 31 citations

The Involvement of a Complex between Formylmethionyl‐tRNA and Initiation Factor IF‐2 in Prokaryotic Initiation

1977 • 31 citations

Prokaryotic initiation factor 2 acts at the level of the 30 S ribosomal subunit

1986 • 30 citations

Carboxyl-terminal amino acid residues in elongation factor G essential for ribosome association and translocation

1994 • 30 citations

Site‐directed mutagenesis of elongation factor Tu

1992 • 30 citations

Structural and functional domains of E coli initiation factor IF2

1991 • 29 citations

Three-dimensional models of the GDP and GTP forms of the guanine nucleotide domain of Escherichia coli elongation factor Tu

1990 • 29 citations

The two main states of the elongating ribosome and the role of the α-sarcin stem-loop structure of 23S RNA

1992 • 27 citations

Antibiotic resistance mechanisms of mutant EF-Tu species inEscherichia coli

1995 • 26 citations

Mechanism of ribosomal translocation

1986 • 26 citations

Substitution of Val20 by Gly in elongation factor Tu

1989 • 26 citations

How many EF-Tu molecules participate in aminocyl-tRNA binding?

1991 • 25 citations

Substitution of proline 82 by threonine induces autophosphorylating activity in GTP-binding domain of elongation factor Tu.

1990 • 25 citations

Substitution of aspartic acid-80, a residue involved in coordination of magnesium, weakens the GTP binding and strongly enhances the GTPase of the G domain of elongation factor Tu

1992 • 25 citations

Nucleotide sequence of theThermus thermophilus HB8gene coding for elongation factor G

1989 • 25 citations

Site-directed mutagenesis of Arg58 and Asp86 of elongation factor Tu from Escherichia coli: effects on the GTPase reaction and aminoacyl-tRNA binding

1995 • 24 citations

Regulation of the Expression of Aminoacyl-tRNA Synthetases and Translation Factors

2013 • 23 citations

Crystallographic studies of elongation factor G

1995 • 22 citations

Characterization of the translational start site for IF2β, a short form of Escherichia coli initiation factor IF2

1990 • 19 citations

Mutation of the Conserved Gly94 and Glyl26 in Elongation Factor Tu from Escherichia Coli. Elucidation of their Structural and Functional Roles

1995 • 19 citations

Mutants of EF‐Tu defective in binding aminoacyl‐tRNA

1996 • 18 citations

Binding of initiation factor 2 and initiator tRNA to the Escherichia coli 30S ribosomal subunit induces allosteric transitions in 16S rRNA

1990 • 18 citations

Topography of the Escherichia coli Initiation Factor 2/fMet-tRNA_f^Met Complex as Studied by Cross-Linking

1996 • 18 citations

Structure of the ternary complex of EF-Tu: macromolecular mimicry in translation

1996 • 17 citations

Mutation of the Conserved Gly94 and Glyl26 in Elongation Factor Tu from Escherichia Coli. Elucidation of their Structural and Functional Roles

1995 • 17 citations

Histidine‐118 of elongation factor Tu: its role in aminoacyl‐tRNA binding and regulation of the GTPase activity

1994 • 17 citations

Mutagenesis of the NH2-terminal domain of elongation factor Tu

1990 • 17 citations

Conformational differences between complexes of elongation factor Tu studied by ¹⁹F‐NMR spectroscopy

1993 • 16 citations

Stoichiometry for the Elongation Factor Tu.cntdot.Aminoacyl-tRNA Complex Switches with Temperature

1995 • 16 citations

Ternary complex between elongation factor Tu•GTP and Phe-tRNAPhe

1993 • 16 citations

In vivo study of engineered G‐domain mutants of Escherichia coli translation initiation factor IF2

1994 • 16 citations

Two GTPs are hydrolysed on two molecules of EF-Tu for each elongation cycle during code translation

1994 • 15 citations

Site-directed mutagenesis of the GDP binding domain of bacterial elongation factor Tu

1989 • 15 citations

Conformational Transition of Polypeptide Chain Elongation Factor G as Determined by Electron Spin Resonance

1976 • 14 citations

Crystals of intact elongation factor Tu from Thermus thermophilus diffracting to high resolution

1991 • 13 citations

Purification and crystallization of the ternary complex of elongation factor Tu:GTP and Phe‐tRNA^Phe

1994 • 13 citations

The role of translocation in ribosomal accuracy. Translocation rates for cognate and noncognate aminoacyl- and peptidyl-tRNAs on Escherichia coli ribosomes.

1987 • 13 citations

Purified internal G-domain of translational initiation factor IF-2 displays guanine nucleotide binding properties

1990 • 13 citations

Functional domains in the Escherichia coli release factors

1993 • 12 citations

Visualization of a Ternary Complex of the Escherichia coli Phe-tRNAPhe and Tu · GTP from Thermus thermophilus by Scanning Transmission Electron Microscopy

1993 • 12 citations

NMR study of the phosphate-binding elements of Escherichia coli elongation factor Tu catalytic domain

1991 • 11 citations

Two GTPs are consumed on EF-Tu per peptide bond in poly(Phe) synthesis, in spite of switching stoichiometry of the EF-Tu·aminoacyl-tRNA complex with temperature

1995 • 11 citations

Effect of propan-2-ol on enzymic and structural properties of elongation factor G

1989 • 10 citations

Structure-function relationships of elongation factor Tu as studied by mutagenesis

1991 • 10 citations

Isolation and stability of ternary complexes of elongation factor Tu, GTP and aminoacyl-tRNA

1991 • 10 citations

The functional and structural roles of residues Gln114 and Glu117 in elongation factor Tu

1990 • 9 citations

Stoichiometry of elongation factor G function in translation

1990 • 9 citations

Formation of a Binary Complex between Elongation Factor G and Guanine Nucleotides

1975 • 9 citations

Stereochemistry and lifetime of the GTP hydrolysis intermediate at the active site of elongation factor Tu from Bacillus stearothermophilus as inferred from the ¹⁷O–⁵⁵Mn superhyperfine interaction

1990 • 8 citations

Elongation Factors in Protein Synthesis

2007 • 8 citations

Osmo‐Expression and Fast Two‐Step Purification of Escherichia Coli Translation Termination Factor RF‐3

1995 • 8 citations

The Allosteric Three-Site Model and the Mechanism of Action of Both Elongation Factors EF-Tu and EF-G

1993 • 8 citations

Domain of E. coli translational initiation factor IF2 homologous to lambda cI repressor and displaying DNA binding activity

1993 • 8 citations

Small clusters of divergent amino acids surrounding the effector domain mediate the varied phenotypes of EF‐G and LepA expression

1995 • 7 citations

Stoichiometry of the EF‐Tu · GTP complex with aminoacyl‐tRNA: ternary of quinternary?

1995 • 6 citations

Peptide Chain Termination

1977 • 6 citations

Tyrosine Residues in the C‐Terminal Domain of the Elongation Factor G Are Essential for Its Interaction with the Ribosome

1980 • 5 citations

The Aminoacyl-tRNA·Elongation Factor Tu·GTP Ternary Complex and Its Role in Aminoacyl-tRNA Selection at the Ribosome

1986 • 3 citations

Topography of the Ternary EF-Tu/GTP/Phe-tRNAPhe Complex as Studied by Crosslinking and Limited Proteolysis

1993 • 1 citations