Role of tyrosine-80 in the stability of kanamycin nucleotidyltransferase analyzed by site-directed mutagenesis

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

A simple method for displaying the hydropathic character of a protein

1982 • 22,471 citations

Measurement of protein using bicinchoninic acid

1985 • 17,516 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

Principles that Govern the Folding of Protein Chains

1973 • 6,771 citations

[2] New M13 vectors for cloning

1983 • 5,402 citations

Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing

1980 • 3,476 citations

Proteins: Structures and Molecular Properties

1986 • 2,694 citations

Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.

1983 • 2,456 citations

Estimation of globular protein secondary structure from circular dichroism

1981 • 2,139 citations

The Solubility of Amino Acids and Two Glycine Peptides in Aqueous Ethanol and Dioxane Solutions

1971 • 1,417 citations

[32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors

1983 • 1,093 citations

Electrostatic Effects in Proteins

1978 • 734 citations

The Stability of Globular Protein

1975 • 648 citations

Thermal stability and protein structure

1979 • 518 citations

Life at high temperatures

1994 • 482 citations

Stereochemical basis of heat stability in bacterial ferredoxins and in haemoglobin A2

1975 • 420 citations

Life at High Temperatures

1985 • 248 citations

Molecular basis of thermostability in the lysozyme from bacteriophage T4

1979 • 212 citations

Enzymatic and nucleotide sequence studies of a kanamycin-inactivating enzyme encoded by a plasmid from thermophilic bacilli in comparison with that encoded by plasmid pUB110

1984 • 154 citations

Effect of single amino acid replacements on the thermal stability of the NH2-terminal domain of phage lambda repressor.

1984 • 153 citations

Thermodynamic stability and point mutations of bacteriophage T4 lysozyme

1984 • 149 citations

Cumulative effect of intragenic amino-acid replacements on the thermostability of a protein

1986 • 128 citations

Effect of a single amino acid substitution on stability of conformation of a protein

1977 • 127 citations

Haemoglobin and myoglobin

1981 • 108 citations

Screening for thermostable mutant of kanamycin nucleotidyltransferase by the use of a transformation system for a thermophile, Bacillus stearothermophilus.

1985 • 101 citations

Purification and characterization of a kanamycin nucleotidyltransferase from plasmid pUB110-carrying cells of Bacillus subtilis

1980 • 55 citations

Effect of amino acid residues on conformational stability in eight mutant proteins variously substituted at a unique position of the tryptophan synthase alpha-subunit.

1984 • 50 citations

A genetic enrichment for mutations constructed by oligodeoxynucleotide-directed mutagenesis

1985 • 45 citations

Effect of single amino acid substitutions on the protease susceptibility of tryptophan synthase a subunit

1985 • 21 citations

Single amino acid replacements affecting the thermostability of kanamycin nucleotidyltransferase

1986 • 12 citations