Affinity purification of histidine-tagged proteins

Antibodies, a laboratory manual

1989 • 12,929 citations

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei

1983 • 11,902 citations

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1986 • 6,284 citations

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1988 • 6,107 citations

Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs

1984 • 3,334 citations

Metal chelate affinity chromatography, a new approach to protein fractionation

1975 • 2,050 citations

New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues

1987 • 1,107 citations

Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent

1988 • 1,030 citations

A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification

1988 • 934 citations

Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus.

1991 • 464 citations

Metal-Affinity Separations: A New Dimension in Protein Processing

1991 • 455 citations

RXR alpha, a promiscuous partner of retinoic acid and thyroid hormone receptors.

1992 • 446 citations

Crystal structure of TFIID TATA-box binding protein

1992 • 410 citations

Octamer binding proteins confer transcriptional activity in early mouse embryogenesis.

1989 • 338 citations

Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies

1991 • 289 citations

Oct-6: a POU transcription factor expressed in embryonal stem cells and in the developing brain.

1990 • 238 citations

Engineering proteins for purification

1990 • 165 citations

Mechanisms of transactivation by retinoic acid receptors

1993 • 156 citations

Amino-terminal domain of NF1 binds to DNA as a dimer and activates adenovirus DNA replication.

1990 • 146 citations

[12] Gene fusions for purpose of expression: An introduction

1990 • 145 citations

Large–Scale Affinity Purification of Human Insulin–Like Growth Factor I from Culture Medium of Escherichia Coli

1987 • 135 citations

Identification of multiple SRF N-terminal phosphorylation sites affecting DNA binding properties.

1992 • 132 citations

Affinity purification of histidine-tagged proteins transiently produced in HeLa cells

1992 • 38 citations

The glucocorticoid receptor hormone binding domain mediates transcriptional activationin vitroin the absence of ligand

1993 • 36 citations