Protein folding in vitro

Protein Folding

1992 • 1,601 citations

The molten globule state as a clue for understanding the folding and cooperativity of globular‐protein structure

1989 • 1,446 citations

Study of the “molten globule” intermediate state in protein folding by a hydrophobic fluorescent probe

1991 • 1,231 citations

DENATURED STATES OF PROTEINS

1991 • 870 citations

Chaperonin-mediated protein folding at the surface of groEL through a 'molten globule'-like intermediate

1991 • 853 citations

Mechanism of acid-induced folding of proteins

1990 • 560 citations

GroE facilitates refolding of citrate synthase by suppressing aggregation

1991 • 486 citations

Characterization of a partly folded protein by NMR methods: studies on the molten globule state of guinea pig .alpha.-lactalbumin

1989 • 473 citations

Protein Aggregation in vitro and in vivo: A Quantitative Model of the Kinetic Competition between Folding and Aggregation

1991 • 440 citations

Protein folding: local structures, domains, subunits, and assemblies

1991 • 432 citations

A 'molten-globule' membrane-insertion intermediate of the pore-forming domain of colicin A

1991 • 416 citations

A kinetic study of the competition between renaturation and aggregation during the refolding of denatured-reduced egg white lysozyme

1991 • 393 citations

Structural description of acid-denatured cytochrome c by hydrogen exchange and 2D NMR

1990 • 306 citations

Thermodynamic study of the apomyoglobin structure

1988 • 299 citations

Chaperonins facilitate the in vitro folding of monomeric mitochondrial rhodanese

1991 • 298 citations

Molten globule intermediates and protein folding

1991 • 281 citations

Evidence for identity between the equilibrium unfolding intermediate and a transient folding intermediate: a comparative study of the folding reactions of .alpha.-lactalbumin and lysozyme

1986 • 254 citations

Demonstration by NMR of folding domains in lysozyme

1991 • 246 citations

Structure and Morphology of Protein Inclusion Bodies in Escherichia Coli

1991 • 238 citations

Contribution of hydration and non-covalent interactions to the heat capacity effect on protein unfolding

1992 • 237 citations

A Kinetic Partitioning Model of Selective Binding of Nonnative Proteins by the Bacterial Chaperone SecB

1991 • 225 citations

Stable submolecular folding units in a non-compact form of cytochrome c

1991 • 220 citations

Early steps in cytochrome c folding probed by time-resolved circular dichroism and fluorescence spectroscopy

1992 • 219 citations

Unfolded proteins, compact states and molten globules

1992 • 199 citations

Probing the stability of a partly folded apomyoglobin intermediate by site-directed mutagenesis

1991 • 179 citations

The folding of an enzyme

1992 • 172 citations

Theory of cooperative transitions in protein molecules. II. Phase diagram for a protein molecule in solution

1989 • 157 citations

Alternatively folded states of an immunoglobulin

1991 • 155 citations

Refolding and aggregation of bovine carbonic anhydrase B: quasi-elastic light scattering analysis

1990 • 155 citations

Thermodynamic characterization of cytochrome c at low pH

1992 • 153 citations

Protein stability: electrostatics and compact denatured states.

1991 • 151 citations

Transient intermediates in the folding of dihydrofolate reductase as detected by far-ultraviolet circular dichroism spectroscopy

1991 • 147 citations

Dissociation of a native dimer to a molten globule monomer

1992 • 147 citations

The molten globule protein conformation probed by disulphide bonds

1991 • 143 citations

Kinetics of disulfide bond reduction in .alpha.-lactalbumin by dithiothreitol and molecular basis of superreactivity of the Cys6-Cys120 disulfide bond

1990 • 137 citations

Role of electrostatic repulsion in the acidic molten globule of cytochrome c

1991 • 137 citations

Calorimetric determination of the energetics of the molten globule intermediate in protein folding: apo-.alpha.-lactalbumin

1991 • 118 citations

Folding of staphylococcal nuclease A studied by equilibrium and kinetic circular dichroism spectra

1991 • 115 citations

Absence of the thermal transition in apo-α-lactalbumin in the molten globule state

1992 • 111 citations

Conformational states of ribulose bisphosphate carboxylase and their interaction with chaperonin 60

1992 • 108 citations

How does protein synthesis give rise to the 3D‐structure?

1991 • 107 citations

Mechanism of polyethylene glycol interaction with the molten globule folding intermediate of bovine carbonic anhydrase B.

1992 • 95 citations

Unfolded proteins, compact states and molten globules

1992 • 94 citations

A water-lipid interface induces a highly dynamic folded state in apocytochrome c and cytochrome c, which may represent a common folding intermediate

1992 • 87 citations

Mechanism of the conformational transition of melittin

1992 • 81 citations

Microcalorimetric studies of conformational transitions of ferricytochrome c in acidic solution

1989 • 73 citations

Anion and pH-dependent conformational transition of an amphiphilic polypeptide

1991 • 69 citations

The three states of globular proteins: Acid denaturation

1991 • 66 citations

Comparison of the nucleotide sequence of cloned human and guinea-pig pre-α-lactalbumin cDNA with that of chick pre-lysozyme cDNA suggests evolution from a common ancestral gene

1982 • 65 citations

Detection and characterization of intermediates in the folding of large proteins by the use of genetically inserted tryptophan probes

1991 • 55 citations

Conformations and forces in protein folding

1992 • 55 citations

Protein aggregation and inclusion body formation in Escherichia coli rpoH mutant defective in heat shock protein induction

1991 • 44 citations

The isolated C-terminal (F2) fragment of the Escherichia coli tryptophan synthase .beta.2-subunit folds into a stable, organized nonnative conformation

1991 • 42 citations

The Pro¹¹⁷ to glycine mutation of staphylococcal nuclease simplifies the unfolding—folding kinetics

1991 • 42 citations

Evidence for an acid-induced molten-globule state in interleukin-2; a fluorescence and circular dichroism study

1991 • 40 citations

Site-directed mutagenesis to probe protein folding: evidence that the formation and aggregation of a bovine growth hormone folding intermediate are dissociable processes

1991 • 37 citations

Use of fluorescence energy transfer to characterize the compactness of the constant fragment of an immunoglobulin light chain in the early stage of folding

1991 • 34 citations

Structure and dynamics of des-pentapeptide-insulin in solution: the molten-globule hypothesis.

1992 • 31 citations

Transient Association of the First Intermediate during the Refolding of Bovine Carbonic Anhydrase B

1992 • 29 citations

Proposed molten globule intermediates in fd phage penetration and assembly

1991 • 24 citations

The mechanism of protein folding

1992 • 24 citations

Hydrophobic core of molten-globule state of bovine carbonic anhydrase B

1991 • 22 citations

Protein folding in vitro and in the cellular environment

1991 • 14 citations

A simplified mechanical model of proteins tested on the globin fold

1991 • 8 citations

Anion-Induced Conformational Change of Apo-Electron-Transferring Flavoprotein1

1992 • 5 citations