Ras effector-homolog region on Rac regulates protein associations in the neutrophil respiratory burst oxidase complex

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

Molecular Switch for Signal Transduction: Structural Differences Between Active and Inactive Forms of Protooncogenic ras Proteins

1990 • 1,037 citations

Activation of the NADPH oxidase involves the small GTP-binding protein p21rac1

1991 • 961 citations

Direct interaction of Ras and the amino-terminal region of Raf-1 in vitro

1993 • 730 citations

Regulation of Phagocyte Oxygen Radical Production by the GTP-Binding Protein Rac 2

1991 • 669 citations

rac, a novel ras-related family of proteins that are botulinum toxin substrates

1989 • 585 citations

Two cytosolic components of the human neutrophil respiratory burst oxidase translocate to the plasma membrane during cell activation.

1990 • 435 citations

Cloning of a 67-kD Neutrophil Oxidase Factor with Similarity to a Noncatalytic Region of p60 ^c-src

1990 • 393 citations

Cytochrome b ₅₅₈ : the Flavin-Binding Component of the Phagocyte NADPH Oxidase

1992 • 384 citations

Neutrophil nicotinamide adenine dinucleotide phosphate oxidase assembly. Translocation of p47-phox and p67-phox requires interaction between p47-phox and cytochrome b558.

1991 • 379 citations

Cytochrome b-245 is a flavocytochrome containing FAD and the NADPH-binding site of the microbicidal oxidase of phagocytes

1992 • 351 citations

Recombinant 47-Kilodalton Cytosol Factor Restores NADPH Oxidase in Chronic Granulomatous Disease

1989 • 341 citations

Unsaturated fatty acids stimulate NADPH-dependent superoxide production by cell-free system derived from macrophages

1984 • 335 citations

Activation of the respiratory burst enzyme from human neutrophils in a cell-free system. Evidence for a soluble cofactor.

1985 • 334 citations

Translocation of Rac correlates with NADPH oxidase activation. Evidence for equimolar translocation of oxidase components.

1993 • 316 citations

Activation of human neutrophil nicotinamide adenine dinucleotide phosphate, reduced (triphosphopyridine nucleotide, reduced) oxidase by arachidonic acid in a cell-free system.

1985 • 315 citations

Reconstitution of neutrophil NADPH oxidase activity in the cell-free system by four components: p67-phox, p47-phox, p21rac1, and cytochrome b-245.

1992 • 265 citations

The effector interactions of p21ras

1993 • 193 citations

Regulation of neutrophil NADPH oxidase activation in a cell-free system by guanine nucleotides and fluoride. Evidence for participation of a pertussis and cholera toxin-insensitive G protein.

1987 • 188 citations

GTP-dependent association of Raf-1 with Ha-Ras: identification of Raf as a target downstream of Ras in mammalian cells.

1993 • 180 citations

The cytosolic components of the respiratory burst oxidase exist as a M(r) approximately 240,000 complex that acquires a membrane-binding site during activation of the oxidase in a cell-free system.

1992 • 150 citations

Multiple forms of myeloperoxidase from human neutrophilic granulocytes: Evidence for differences in compartmentalization, enzymatic activity, and subunit structure

1983 • 134 citations

Participation of the small molecular weight GTP-binding protein Rac1 in cell-free activation and assembly of the respiratory burst oxidase. Inhibition by a carboxyl-terminal Rac peptide.

1994 • 90 citations

Activation of the respiratory burst oxidase in neutrophils: On the role of membrane-derived second messengers, Ca++, and protein kinase C

1988 • 83 citations

Production of recombinant cytochrome b558 allows reconstitution of the phagocyte NADPH oxidase solely from recombinant proteins

1993 • 76 citations

The respiratory burst oxidase of human neutrophils. Guanine nucleotides and arachidonate regulate the assembly of a multicomponent complex in a semirecombinant cell-free system.

1993 • 67 citations

Diradylglycerol synergizes with an anionic amphiphile to activate superoxide generation and phosphorylation of p47phox in a cell-free system from human neutrophils.

1990 • 67 citations

Reversible activation of NADPH oxidase in membranes of HL-60 human leukemic cells

1987 • 56 citations

Absolute requirement for GTP in activation of human neutrophil NADPH oxidase in a cell-free system: role of ATP in regenerating GTP.

1992 • 52 citations

Nucleoside triphosphate requirements for superoxide generation and phosphorylation in a cell-free system from human neutrophils. Sodium dodecyl sulfate and diacylglycerol activate independently of protein kinase C.

1991 • 47 citations

Reconstitution and characterization of the human neutrophil respiratory burst oxidase using recombinant p47-phox, p67-phox and plasma membrane

1992 • 39 citations

Activation of superoxide radical anion generating oxidase of bovine neutrophils in a cell-free system. Interaction of a cytosolic factor with the plasma membrane and control by G nucleotides

1989 • 36 citations