Two-Dimensional Electrophoresis of Membrane Proteins Using Immobilized pH Gradients

A simple method for displaying the hydropathic character of a protein

1982 • 22,471 citations

Escherichia coli and Salmonella :cellular and molecular biology

1996 • 8,124 citations

Phase separation of integral membrane proteins in Triton X-114 solution.

1981 • 3,366 citations

Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum.

1982 • 1,765 citations

Proteome and proteomics: New technologies, new concepts, and new words

1998 • 1,036 citations

Isoelectric focusing in immobilized pH gradients: Principle, methodology and some applications

1982 • 963 citations

The Gross Conformation of Protein-Sodium Dodecyl Sulfate Complexes

1970 • 935 citations

Two-dimensional gel electrophoresis of membrane proteins

1976 • 702 citations

Binding of Dodecyl Sulfate to Proteins at High Binding Ratios. Possible Implications for the State of Proteins in Biological Membranes

1970 • 684 citations

Methods for increasing the resolution of two-dimensional protein electrophoresis

1988 • 650 citations

Isoelectric Focusing: Theory, Methodology and Applications

1983 • 563 citations

Extraction of membrane proteins by differential solubilization for separation using two‐dimensional gel electrophoresis

1998 • 499 citations

Improvement of the solubilization of proteins in two‐dimensional electrophoresis with immobilized pH gradients

1997 • 484 citations

Proteome Research: New Frontiers in Functional Genomics

1997 • 484 citations

Sample application by in‐gel rehydration improves the resolution of two‐dimensional electrophoresis with immobilized pH gradients in the first dimension

1994 • 367 citations

Immobilized pH Gradients: Theory and Methodology

1990 • 363 citations

On the Structural Stability and Solvent Denaturation of Proteins

1970 • 346 citations

2-D Proteome Analysis Protocols

1998 • 342 citations

New zwitterionic detergents improve the analysis of membrane proteins by two‐dimensional electrophoresis

1998 • 334 citations

Solubilization of proteins for electrophoretic analyses

1996 • 316 citations

Improved and simplified in‐gel sample application using reswelling of dry immobilized pH gradients

1997 • 309 citations

The Binding of Detergents to Proteins

1971 • 267 citations

Recent developments in two-dimensional gel electrophoresis with immobilized pH gradients: Wide pH gradients up to pH 12, longer separation distances and simplified procedures

1999 • 251 citations

[10] Reductive cleavage of cystine disulfides with tributylphosphine

1977 • 249 citations

Improved protein solubility in two‐dimensional electrophoresis using tributyl phosphine as reducing agent

1998 • 241 citations

Proteomics and automation

1999 • 209 citations

Comparison of yeast cell protein solubilization procedures for two-dimensional electrophoresis

1999 • 202 citations

Two‐dimensional gel electrophoresis for proteome projects: The effects of protein hydrophobicity and copy number

1998 • 199 citations

Micropreparative two‐dimensional electrophoresis allowing the separation of samples containing milligram amounts of proteins

1993 • 191 citations

Surfactants in membrane solubilisation

1999 • 184 citations

Positional reproducibility of protein spots in two‐dimensional polyacrylamide gel electrophoresis using immobilised pH gradient isoelectric focusing in the first dimension: An interlaboratory comparison

1994 • 151 citations

Towards the recovery of hydrophobic proteins on two-dimensional electrophoresis gels

1999 • 150 citations

Preparative two‐dimensional gel electrophoresis of membrane proteins

1997 • 144 citations

Two‐dimensional electrophoresis of membrane proteins: A current challenge for immobilized pH gradients

1997 • 138 citations

The use of a zwitterionic detergent in two-dimensional gel electrophoresis of trout liver microsomes

1983 • 128 citations

Two‐dimensional electrophoresis of proteins in an immobilized pH 4–12 gradient

1998 • 123 citations

Extraction ofEscherichia coli proteins with organic solvents prior to two-dimensional electrophoresis

1999 • 119 citations

Membrane protein analysis by isoelectric focusing in immobilized pH gradients

1985 • 118 citations

Identification of the major membrane and core proteins of vaccinia virus by two-dimensional electrophoresis

1996 • 115 citations

The Detection of Enzyme Activity Following Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis

1998 • 79 citations

Proteome in Perspective

1998 • 77 citations

Effect of 2-mercaptoethanol on pH gradients in isoelectric focusing

1982 • 68 citations

Amidosulfobetaines, a family of detergents with improved solubilization properties: Application for isoelectric focusing under denaturing conditions

1990 • 67 citations

Isoelectric focusing and non‐isoelectric precipitation of ferritin in immobilized pH gradients: An improved protocol overcoming protein‐matrix interactions

1987 • 58 citations

Immobilized pH gradients: Effect of salts, added carrier ampholytes and voltage gradients on protein patterns

1988 • 56 citations

Proteomic ‘contigs’ of Ochrobactrum anthropi, application of extensive pH gradients

1997 • 55 citations

Construction of a directory of tobacco plasma membrane proteins by combined two‐dimensional gel electrophoresis and protein sequencing

1997 • 46 citations

Resolution of fibronectin and other uncharacterized proteins by two-dimensional polyacrylamide electrophoresis with thiourea

1998 • 45 citations

High-resolution two-dimensional electrophoresis of plant proteins

1988 • 42 citations

Studies on Adrenal Steroid Hydroxylases

1970 • 41 citations

Stable storage conditions of immobiline chemicals for isoelectric focusing

1988 • 39 citations

Additives for immobilized pH gradient two-dimensional separation of particulate material: Comparison between commercial and new synthetic detergents

1987 • 27 citations

Analytical IPG-Dalt

2003 • 26 citations

Dodecyl maltoside-sodium dodecyl sulfate two-dimensional polyacrylamide gel electrophoresis of chloroplast thylakoid membrane proteins

1988 • 24 citations

A universal method for two-dimensional polyacrylamide gel electrophoresis of membrane proteins using isoelectric focusing on immobilized pH gradients in the first dimension

1991 • 22 citations

Polyethyleneglycol methacrylate 200 as an electrophoresis matrix in hydroorganic solvents

1992 • 18 citations

Biochemical analysis of membrane proteins from an early maturation stage of phagosomes

1999 • 16 citations

Quantitative Reduction and Alkylation of Wool

1971 • 16 citations

Isoelectric focusing in immobilized pH gradients of the glucose transporter from human red cell membranes

1993 • 11 citations

Two‐dimensional electrophoresis of membrane proteins: Separation of myelin proteins

1990 • 11 citations

Two-Dimensional Electrophoresis and Densitometry Analysis of Solubilized Bovine Sperm Plasma Membrane Proteins Detected by Silver Staining and Radioiodination

1992 • 9 citations

Biochemical characterization by two‐dimensional electrophoresis of lymphocyte antigens involved in cell‐to‐cell or cell‐to‐matrix adhesion

1991 • 3 citations