In Vitro Dissociation of BiP-Peptide Complexes Requires a Conformational Change in BiP after ATP Binding but Does Not Require ATP Hydrolysis

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

Protein folding in the cell

1992 • 4,138 citations

An hsp70-like protein in the ER: Identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein

1986 • 1,457 citations

Three-dimensional structure of the ATPase fragment of a 70K heat-shock cognate protein

1990 • 1,035 citations

Escherichia coli DnaJ and GrpE heat shock proteins jointly stimulate ATPase activity of DnaK.

1991 • 835 citations

Peptide Binding and Release by Proteins Implicated as Catalysts of Protein Assembly

1989 • 763 citations

Loss of BiP/GRP78 function blocks translocation of secretory proteins in yeast.

1990 • 435 citations

ATP-induced protein Hsp70 complex dissociation requires K+ but not ATP hydrolysis

1993 • 366 citations

Similarity of the three-dimensional structures of actin and the ATPase fragment of a 70-kDa heat shock cognate protein.

1991 • 352 citations

Interaction of hsp70 with unfolded proteins: effects of temperature and nucleotides on the kinetics of binding.

1991 • 332 citations

Different conformations for the same polypeptide bound to chaperones DnaK and GroEL

1992 • 285 citations

The ATPase core of a clathrin uncoating protein.

1987 • 259 citations

The Escherichia coli DnaK chaperone, the 70-kDa heat shock protein eukaryotic equivalent, changes conformation upon ATP hydrolysis, thus triggering its dissociation from a bound target protein

1991 • 245 citations

Eukaryotic homologues of Escherichia coli dnaJ: a diverse protein family that functions with hsp70 stress proteins.

1993 • 225 citations

DnaK as a thermometer: threonine-199 is site of autophosphorylation and is critical for ATPase activity.

1991 • 218 citations

Interconversion of three differentially modified and assembled forms of BiP.

1992 • 180 citations

Interaction of heavy chain binding protein (BiP/GRP78) with adenine nucleotides.

1989 • 176 citations

Identification of the peptide binding domain of hsc70. 18-Kilodalton fragment located immediately after ATPase domain is sufficient for high affinity binding.

1993 • 166 citations

The chaperone function of DnaK requires the coupling of ATPase activity with substrate binding through residue E171.

1994 • 118 citations

Mutations within the nucleotide binding site of immunoglobulin-binding protein inhibit ATPase activity and interfere with release of immunoglobulin heavy chain.

1993 • 113 citations

In vivo expression of mammalian BiP ATPase mutants causes disruption of the endoplasmic reticulum.

1995 • 100 citations

Structural basis of the 70-kilodalton heat shock cognate protein ATP hydrolytic activity. I. Kinetic analyses of active site mutants.

1994 • 100 citations

The nucleotide sequence encoding the hamster 78-kDa glucose-regulated protein (GRP78) and its conservation between hamster and rat

1987 • 93 citations

YGE1 is a yeast homologue of Escherichia coli grpE and is required for maintenance of mitochondrial functions

1994 • 92 citations

Characterization of the Nucleotide Binding Properties and ATPase Activity of Recombinant Hamster BiP Purified from Bacteria

1995 • 89 citations

Interactions of liver Grp78 and Escherichia coli recombinant Grp78 with ATP: multiple species and disaggregation.

1992 • 73 citations

DnaK mutants defective in ATPase activity are defective in negative regulation of the heat shock response: expression of mutant DnaK proteins results in filamentation

1994 • 68 citations

Threonine 204 of the chaperone protein Hsc70 influences the structure of the active site, but is not essential for ATP hydrolysis.

1993 • 62 citations

Partial loss of function mutations in DnaK, the Escherichia coli homologue of the 70-kDa heat shock proteins, affect highly conserved amino acids implicated in ATP binding and hydrolysis.

1992 • 54 citations

The immunoglobulin-binding protein in vitro autophosphorylation site maps to a threonine within the ATP binding cleft but is not a detectable site of in vivo phosphorylation

1993 • 30 citations

Dissociation of glucose-regulated protein Grp78 and Grp78-IgE Fc complexes by ATP.

1993 • 24 citations

Affinity-purification and identification of GrpE homologues from mammalian mitochondria

1995 • 18 citations