Site-directed mutagenesis of the GTP-binding domain of β-tubulin

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

The Protein Kinase Family: Conserved Features and Deduced Phylogeny of the Catalytic Domains

1988 • 5,115 citations

Dynamic instability of microtubule growth

1984 • 3,187 citations

Prediction of the occurrence of the ADP-binding βαβ-fold in proteins, using an amino acid sequence fingerprint

1986 • 1,172 citations

Refined crystal structure of the triphosphate conformation of H-ras p21 at 1.35 A resolution: implications for the mechanism of GTP hydrolysis.

1990 • 1,077 citations

Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies.

1988 • 985 citations

Turbidimetric studies of the in vitro assembly and disassembly of porcine neurotubules

1974 • 814 citations

GTP-binding domain: three consensus sequence elements with distinct spacing.

1987 • 602 citations

TCP1 complex is a molecular chaperone in tubulin biogenesis

1992 • 457 citations

Effects of Mg2+ and the beta gamma-subunit complex on the interactions of guanine nucleotides with G proteins.

1987 • 359 citations

Determination of cell division axes in the early embryogenesis of Caenorhabditis elegans.

1987 • 346 citations

Complete amino acid sequence of beta-tubulin from porcine brain.

1981 • 303 citations

Crystal structure of the complex of phosphofructokinase from Escherichia coli with its reaction products

1988 • 270 citations

Peptide sequences that target proteins for enhanced degradation during serum withdrawal.

1988 • 238 citations

Microtubule Dynamics and Kinetochore Function in Mitosis

1988 • 208 citations

Dominant effects of tubulin overexpression in Saccharomyces cerevisiae.

1989 • 191 citations

Modification of nuclear lamin proteins by a mevalonic acid derivative occurs in reticulocyte lysates and requires the cysteine residue of the C-terminal CXXM motif.

1989 • 183 citations

Localization of the 90-kDa heat shock protein-binding site within the hormone-binding domain of the glucocorticoid receptor by peptide competition

1991 • 165 citations

NMR studies of the magnesium-ATP binding site of adenylate kinase and of a 45-residue peptide fragment of the enzyme

1985 • 160 citations

An unusual active site identified in a family of zinc metalloendopeptidases.

1992 • 158 citations

A mutation that alters the nucleotide specificity of elongation factor Tu, a GTP regulatory protein.

1987 • 153 citations

Colchicine inhibition of microtubule assembly via copolymer formation.

1979 • 147 citations

Mg2+ dependence of guanine nucleotide binding to tubulin.

1987 • 140 citations

Modelling the ATP‐binding site of oncogene products, the epidermal growth factor receptor and related proteins

1984 • 121 citations

Homologies in the primary structure of GTP-binding proteins: the nucleotide-binding site of EF-Tu and p21.

1984 • 109 citations

Sequence of one α- and two β-tubulin genes of Tetrahymena pyriformis

1988 • 100 citations

Isolation of separate mRNAs for α- and β-Tubulin and characterization of the corresponding in vitro translation products

1978 • 99 citations

The GTP-binding peptide of beta-tubulin. Localization by direct photoaffinity labeling and comparison with nucleotide-binding proteins.

1988 • 85 citations

Role of GTP hydrolysis in microtubule polymerization: evidence for a coupled hydrolysis mechanism

1990 • 81 citations

Use of a GTP photoaffinity probe to resolve aspects of the mechanism of tubulin polymerization.

1979 • 80 citations

A simple and rapid purification of commercial trypsin and chymotrypsin by reverse-phase high-performance liquid chromatography

1982 • 80 citations

Soluble 14-kDa beta-galactoside-specific bovine lectin. Evidence from mutagenesis and proteolysis that almost the complete polypeptide chain is necessary for integrity of the carbohydrate recognition domain.

1991 • 79 citations

Determination of free and bound microtubular protein and guanine nucleotide under equilibrium conditions

1979 • 77 citations

A model of the nucleotide‐binding site in tubulin

1987 • 77 citations

Comparison of active sites of some microbial ribonucleases: structural basis for guanylic specificity

1990 • 72 citations

Tubulin sequence region beta 155-174 is involved in binding exchangeable guanosine triphosphate.

1987 • 61 citations

Direct photoaffinity labeling of tubulin with guanosine 5'-triphosphate

1985 • 60 citations

Different effects of tubulin ligands on the intrachain cross-linking of beta 1-tubulin.

1984 • 58 citations

Location of two cysteines in brain β1-tubulin that can be cross-linked after removal of exchangeable GTP

1987 • 58 citations

Structural determination of the functional sites of E. coli elongation factor Tu

1990 • 57 citations

Mechanism of GTP hydrolysis in tubulin polymerization: characterization of the kinetic intermediate microtubule-GDP-Pi using phosphate analogs

1989 • 55 citations

Maytansine inhibits nucleotide binding at the exchangeable site of tubulin

1985 • 53 citations

Mechanism of the microtubule GTPase reaction.

1990 • 50 citations

Assembly of proteolytically cleaved tubulin

1983 • 41 citations

Limited proteolysis of tubulin: nucleotide stabilizes an active conformation

1983 • 35 citations

Expression of a human .alpha.-tubulin: properties of the isolated subunit

1988 • 32 citations

Differential effects of magnesium on tubulin-nucleotide interactions

1985 • 30 citations

Interaction of 6-mercapto-GTP with bovine brain tubulin. Equilibrium aspects.

1984 • 26 citations

Translation of beta-tubulin mRNA in vitro generates multiple molecular forms.

1988 • 25 citations

Expression of the alpha and beta tubulin genes of the African trypanosome in Escherichia coli

1987 • 17 citations

A second consensus sequence of ATP‐requiring proteins resides in the 21‐kDa C‐terminal segment of myosin subfragment 1

1990 • 16 citations

Alpha-tubulin influences nucleotide binding to beta-tubulin: an assay using picomoles of unpurified protein.

1990 • 13 citations

Kinetics of β-tubulin exchange following translation

1989 • 13 citations

Effect of guanine nucleotides on the hydrophobic interaction of tubulin

1990 • 11 citations

Tubulin-nucleotide interactions. Effects of removal of exchangeable guanine nucleotide on protein conformation and microtubule assembly

1987 • 4 citations