Cloning vectors for expression of cDNA libraries in mammalian cells.

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

Selective extraction of polyoma DNA from infected mouse cell cultures

1967 • 5,357 citations

Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes

1986 • 4,785 citations

A simple and very efficient method for generating cDNA libraries

1983 • 4,606 citations

Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs

1984 • 3,334 citations

Multiple nuclear factors interact with the immunoglobulin enhancer sequences

1986 • 2,771 citations

Gene transfer into mouse lyoma cells by electroporation in high electric fields.

1982 • 2,413 citations

The isolation of structural genes from libraries of eucaryotic DNA

1978 • 1,877 citations

DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.

1979 • 1,339 citations

High-Efficiency Cloning of Full-Length cDNA

1982 • 1,309 citations

Redesign of Retrovirus Packaging Cell Lines To Avoid Recombination Leading to Helper Virus Production

1986 • 1,276 citations

Human GM-CSF: Molecular Cloning of the Complementary DNA and Purification of the Natural and Recombinant Proteins

1985 • 1,253 citations

Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.

1981 • 1,048 citations

Construction and applications of a highly transmissible murine retrovirus shuttle vector

1984 • 1,020 citations

Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure.

1987 • 949 citations

Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation.

1984 • 889 citations

Expression of a foreign gene in myeloid and lymphoid cells derived from multipotent haematopoietic precursors

1985 • 598 citations

A drosophila RNA polymerase II transcription factor binds to the regulatory site of an hsp 70 gene

1984 • 534 citations

A vector that replicates as a plasmid and can be efficiently selected in B-lymphoblasts transformed by Epstein-Barr virus.

1985 • 445 citations

Mapping temperature-sensitive and host-range mutations of adenovirus type 5 by marker rescue

1978 • 408 citations

Bifunctional messenger RNAs in eukaryotes

1986 • 405 citations

The isolation and sequence of the gene encoding T8: A molecule defining functional classes of T lymphocytes

1985 • 325 citations

Polyethylene glycol-induced mammalian cell hybridization: Effect of polyethylene glycol molecular weight and concentration

1976 • 300 citations

Deletion mutants of bacteriophage lambda

1971 • 289 citations

Expression of human class II major histocompatibility complex antigens using retrovirus vectors.

1987 • 257 citations

Construction of coliphage lambda Charon vectors with BamHI cloning sites

1980 • 230 citations

Isolation of the chicken thymidine kinase gene by plasmid rescue

1980 • 208 citations

Polyomavirus enhancer contains multiple redundant sequence elements that activate both DNA replication and gene expression.

1985 • 206 citations

A Vector That Replicates as a Plasmid and Can Be Efficiently Selected in B-Lymphoblasts Transformed by Epstein-Barr Virus

1985 • 187 citations

Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell growth-factor activity after transfection of monkey cells.

1985 • 178 citations

[35] Use of the λ phage promoter PL to promote gene expression in hybrid plasmid cloning vehicles

1979 • 127 citations

Isolation of the gene encoding the human T-lymphocyte differentiation antigen Leu-2 (T8) by gene transfer and cDNA subtraction.

1984 • 108 citations

Translation of insulin-related polypeptides from messenger RNAs with tandemly reiterated copies of the ribosome binding site

1983 • 105 citations

Eukaryotic viral vectors

1987 • 96 citations

Molecular cloning and structural analysis of murine thymidine kinase genomic and cDNA sequences.

1985 • 96 citations

Sequences in the polyomavirus DNA regulatory region involved in viral DNA replication and early gene expression

1985 • 83 citations

Cell-specified expression of a selectable hybrid gene.

1984 • 69 citations

Bacteriophage lambda vector for transducing a cDNA clone library into mammalian cells.

1985 • 57 citations

Molecular Cloning and Structural Analysis of Murine Thymidine Kinase Genomic and cDNA Sequences

1985 • 28 citations

Bacteriophage Lambda Vector for Transducing a cDNA Clone Library into Mammalian Cells

1985 • 12 citations