Rapid fluorimetric assay for the detection of the peptidyl α-amdating enzyme intermediate using high-performance liquid chromatography

Identification in pituitary tissue of a peptide alpha-amidation activity that acts on glycine-extended peptides and requires molecular oxygen, copper, and ascorbic acid.

1983 • 363 citations

The Enzymology and Intracellular Organization of Peptide Precursor Processing: The Secretory Vesicle Hypothesis

1985 • 194 citations

NEUROPEPTIDES: MULTIPLE MOLECULAR FORMS, METABOLIC PATHWAYS, AND RECEPTORS

1986 • 177 citations

The 108-kDa peptidylglycine α-amidating monooxygenase precursor contains two separable enzymatic activities involved in peptide amidation

1990 • 140 citations

A novel enzyme from bovine neurointermediate pituitary catalyzes dealkylation of .alpha.-hydroxyglycine derivatives, thereby functioning sequentially with peptidylglycine .alpha.-amidating monooxygenase in peptide amidation

1990 • 138 citations

Enzymatic peptidyl .alpha.-amidation proceeds through formation of an .alpha.-hydroxyglycine intermediate

1989 • 103 citations

The reaction product of peptidylglycine alpha-amidating enzyme is a hydroxyl derivative at alpha-carbon of the carboxyl-terminal glycine.

1990 • 85 citations

Peptide C-terminal α-amidating enzyme purified to homogeneity from Xenopuslaevis skin

1986 • 77 citations

Peptidylglycine α-amidating reaction: Evidence for a two-step mechanism involving a stable intermediate at neutral pH

1990 • 76 citations

Two enzymes concerned in peptide hormone α-amidation are synthesized from a single mRNA

1990 • 69 citations

Elucidation of amidating reaction mechanism by frog amidating enzyme, peptidylglycine alpha-hydroxylating monooxygenase, expressed in insect cell culture.

1990 • 67 citations

Nonenzymatic peptide alpha-amidation. Implications for a novel enzyme mechanism.

1985 • 66 citations

Developmental regulation of peptidylglycine α-amidating monooxygenase (PAM) in rat heart atrium and ventricle

1989 • 63 citations

Membrane-associated peptidylglycine alpha-amidating monooxygenase in the heart.

1988 • 63 citations

Formation of the COOH‐terminal amide group of thyrotropin‐releasing‐factor

1983 • 58 citations

Formation of peptide amides by peptidylglycine .alpha.-amidating monooxygenase: a new assay and stereochemistry of hydrogen loss

1988 • 55 citations

The amidating enzyme in pituitary will accept a peptide with c-terminal d-alanine as substrate

1983 • 49 citations

Purification of a peptidylglycine α-amidating enzyme from transplantable rat medullary thyroid carcinomas

1988 • 46 citations

A fluorometric assay for peptidyl α-amidation activity using high-performance liquid chromatography

1988 • 45 citations

A fluorometric assay for HIV-protease activity using high-performance liquid chromatography

1990 • 39 citations

Detection and partial characterization of an amidating enzyme in skin secretion of Xenopus laevis

1986 • 29 citations

Purification and characterization of functional recombinant alpha-amidating enzyme secreted from mammalian cells.

1990 • 23 citations

Recombinant type A rat 75-kDa α-amidating enzyme catalyzes the conversion of glycine-extended peptides to peptide amides via an α-hydroxyglycine intermediate

1991 • 17 citations

A colorimetric assay for measuring peptidylglycine α-amidating monooxygenase using high-performance liquid chromatography

1991 • 15 citations

A rapid fluorometric assay for N-terminal glutaminyl cyclase activity using high-performance liquid chromatography

1988 • 14 citations

Methods for the carboxyl-terminal fluorescent labeling of peptides using solid phase peptide synthesis

1989 • 9 citations

Determination of peptidylglycine α-amidating monooxygenase activity in human serum by thin-layer chromatography

1991 • 5 citations

ONTOGENY OF PEPTIDYLGLYCINE alpha-AMIDATING MONOOXYGENASE ACTIVITY IN RAPIDLY MINERALIZING BONE FROM NEONATAL MOUSE

1989 • 5 citations

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