Cloning and Prokaryotic Expression of a Biologically Active Human Placental Aldose Reductase

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

A comprehensive set of sequence analysis programs for the VAX

1984 • 14,824 citations

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

Efficient isolation of genes by using antibody probes.

1983 • 2,065 citations

Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli

1988 • 1,049 citations

Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli

1983 • 810 citations

The Aldo-Keto Reductase Superfamily

1989 • 455 citations

Regulation of ribosomal RNA promoters with a synthetic lac operator.

1984 • 451 citations

Efficient oligonucleotide-directed construction of mutations in expression vectors by the gapped duplex DNA method using alternating selectable markers

1989 • 321 citations

Aldose Reductase Inhibition Improves Nerve Conduction Velocity in Diabetic Patients

1983 • 314 citations

Herpes Simplex Virus Type-1 Glycoprotein D Gene: Nucleotide Sequence and Expression inEscherichia coli

1982 • 285 citations

RCapillaries: Basement Membrane Thickening by Galactosemia Prevented with Aldose Reductase Inhibitor

1983 • 265 citations

Spacing of the -10 and -35 regions in the tac promoter. Effect on its in vivo activity.

1985 • 245 citations

Aldose reductase in diabetic complications of the eye

1979 • 237 citations

The Pharmacology of Aldose Reductase Inhibitors

1985 • 210 citations

The T7 phage gene 10 leader RNA, a ribosome-binding site that dramatically enhances the expression of foreign genes in Escherichia coli

1988 • 189 citations

Aldose reductase and ϱ‐crystallin belong to the same protein superfamily as aldehyde reductase

1987 • 175 citations

Glomerular Polyol Accumulation in Diabetes and its Prevention by Oral Sorbinil

1984 • 160 citations

Structural similarity of bovine lung prostaglandin F synthase to lens epsilon-crystallin of the European common frog.

1988 • 156 citations

The Effect of Aldose Reductase Inhibition on Motor Nerve Conduction Velocity in Diabetic Rats

1982 • 154 citations

Characterization of cDNA coding for human factor XIIIa.

1986 • 126 citations

Cloning and Sequence Determination of Human Placental Aldose Reductase Gene

1989 • 101 citations

High–Level Expression, Efficient Secretion and Folding of Human Growth Hormone in Escherichia coli

1986 • 101 citations

Chicken ovalbumin is synthesized and secreted by Escherichia coli.

1978 • 100 citations

An ‘internal” signal sequence directs secretion and processing of proinsulin in bacteria

1981 • 68 citations

Aldose reductase in diabetic cataracts

1981 • 50 citations

Immunohistochemical location of placental proteins (PP8, 9, 10, 11, 12) in human term placentae

1980 • 46 citations