Active-site mutations of diphtheria toxin: effects of replacing glutamic acid-148 with aspartic acid, glutamine, or serine

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

[32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors

1983 • 1,093 citations

The tac promoter: a functional hybrid derived from the trp and lac promoters.

1983 • 1,001 citations

Structure of exotoxin A of Pseudomonas aeruginosa at 3.0-Angstrom resolution.

1986 • 517 citations

Determining the Chemical Mechanisms of Enzyme‐Catalyzed Reactions by Kinetic Studies

1977 • 340 citations

The use of phosphorothioate-modified DNA in restriction enzyme reactions to prepare nicked DNA

1985 • 247 citations

Active site of Pseudomonas aeruginosa exotoxin A. Glutamic acid 553 is photolabeled by NAD and shows functional homology with glutamic acid 148 of diphtheria toxin.

1987 • 213 citations

The Intrinsic Pk_A-Values of Functional Groups in Enzymes: Improper Deductions from the Ph-Dependence of Steady-State Parameter

1976 • 168 citations

NAD binding site of diphtheria toxin: identification of a residue within the nicotinamide subsite by photochemical modification with NAD.

1984 • 150 citations

Interaction of Fragment A from Diphtheria Toxin with Nicotinamide Adenine Dinucleotide

1974 • 147 citations

Exotoxin A of Pseudomonas aeruginosa: substitution of glutamic acid 553 with aspartic acid drastically reduces toxicity and enzymatic activity

1987 • 115 citations

The mechanism of ADP-ribosylation of elongation factor 2 catalyzed by fragment a from diphtheria toxin

1977 • 99 citations

Diphtheria toxin. Effect of substituting aspartic acid for glutamic acid 148 on ADP-ribosyltransferase activity.

1985 • 96 citations

ADP-Ribose Transfer Reactions

1989 • 86 citations

Photoaffinity labeling of diphtheria toxin fragment A with NAD: structure of the photoproduct at position 148.

1985 • 86 citations

Amino acid sequence homology between the enzymic domains of diphtheria toxin and Pseudomonas aeruginosa exotoxin A

1988 • 65 citations

Exotoxin A of Pseudomonas aeruginosa: active, cloned toxin is secreted into the periplasmic space of Escherichia coli

1987 • 65 citations

Histidine 21 Is at the NAD+ Binding Site of Diphtheria Toxin

1989 • 61 citations

Photolabeling of Glu-129 of the S-1 subunit of pertussis toxin with NAD

1989 • 59 citations

Mapping the enzymatic active site of Pseudomonas aeruginosa exotoxin A

1988 • 58 citations

Covalent adducts of lactate oxidase. Photochemical formation and structure identification.

1979 • 57 citations

The photochemical addition of alcohols to purine

1968 • 57 citations

Toxoid of Pseudomonas aeruginosa exotoxin A generated by deletion of an active-site residue

1988 • 55 citations

Photoinduced reactions of anthraquinone antitumor agents with peptides and nucleic acid bases: An electron spin resonance and spin trapping study

1985 • 53 citations

Expression of a mutant, full-length form of diphtheria toxin in Escherichia coli

1987 • 50 citations

Streptococcal M protein size mutants occur at high frequency within a single strain.

1986 • 47 citations

Cloned Fragment A of Diphtheria Toxin Is Expressed and Secreted into the Periplasmic Space of Escherichia coli K12

1983 • 46 citations

Molecular cloning and expression of gene fragments from corynebacteriophage beta encoding enzymatically active peptides of diphtheria toxin

1983 • 42 citations

Identification of an active‐site residue in subunit S1 of pertussis toxin by photocrosslinking to NAD

1989 • 41 citations

Restoration of enzymic activity and cytotoxicity of mutant, E553C, Pseudomonas aeruginosa exotoxin A by reaction with iodoacetic acid.

1988 • 40 citations

Localization of the active site of diphtheria toxin

1988 • 30 citations

A novel photo-induced methylation of pyrimidines and condensed pyrimidine compounds

1967 • 24 citations

Cloned diphtheria toxin fragment A is expressed from the tox promoter and exported to the periplasm by the SecA apparatus of Escherichia coli K12.

1983 • 24 citations

Photolabelling of mutant forms of the S1 subunit of pertussis toxin with NAD+

1990 • 22 citations

Photochemical, alkylation of nitrogen heteroaromatics by carboxylic acids under decarboxylation

1967 • 20 citations