Construction and analysis of chromosomal Clostridium difficile mutants

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Basic local alignment search tool

1990 • 87,358 citations

Molecular cloning: A laboratory manual

1990 • 85,659 citations

CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice

1994 • 63,397 citations

Molecular cloning : a laboratory manual

2001 • 30,392 citations

Experiments in molecular genetics

1972 • 28,260 citations

Linear Models and Empirical Bayes Methods for Assessing Differential Expression in Microarray Experiments

2004 • 11,620 citations

A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria

1983 • 7,336 citations

An Epidemic, Toxin Gene–Variant Strain ofClostridium difficile

2005 • 2,004 citations

A Predominantly Clonal Multi-Institutional Outbreak ofClostridium difficile–Associated Diarrhea with High Morbidity and Mortality

2005 • 1,938 citations

Over-production of Proteins inEscherichia coli: Mutant Hosts that Allow Synthesis of some Membrane Proteins and Globular Proteins at High Levels

1996 • 1,892 citations

Toxin production by an emerging strain of Clostridium difficile associated with outbreaks of severe disease in North America and Europe

2005 • 1,422 citations

Use of within-array replicate spots for assessing differential expression in microarray experiments

2005 • 1,363 citations

Glucosylation of Rho proteins by Clostridium difficile toxin B

1995 • 1,025 citations

Health Care Costs and Mortality Associated with Nosocomial Diarrhea Due to Clostridium difficile

2002 • 931 citations

A versatile quick-prep of genomic DNA from Gram-positive bacteria

1995 • 721 citations

The Enterotoxin from Clostridium difficile (ToxA) Monoglucosylates the Rho Proteins

1995 • 478 citations

VIRULENCE GENES OFCLOSTRIDIUM PERFRINGENS

1998 • 347 citations

Antibiotics and hospital-acquired Clostridium difficile-associated diarrhoea: a systematic review

2003 • 308 citations

The Spectrum of Pseudomembranous Enterocolitis and Antibiotic-Associated Diarrhea

2002 • 291 citations

Regulated transcription of Clostridium difficile toxin genes

1998 • 288 citations

Conjugative transfer of clostridial shuttle vectors from Escherichia coli to Clostridium difficile through circumvention of the restriction barrier

2002 • 245 citations

Regulation of toxin synthesis in Clostridium difficile by an alternative RNA polymerase sigma factor

2001 • 245 citations

Generation of an erythromycin-sensitive derivative of Clostridium difficile strain 630 (630Δerm) and demonstration that the conjugative transposon Tn916ΔE enters the genome of this strain at multiple sites

2005 • 233 citations

Northern, Morphological, and Fermentation Analysis of spo0A Inactivation and Overexpression in Clostridium acetobutylicum ATCC 824

2002 • 226 citations

Pathogenesis of Clostridium difficile infection

1998 • 223 citations

High Genetic Variability of the agr Locus in Staphylococcus Species

2002 • 204 citations

A novel type of conserved DNA-binding domain in the transcriptional regulators of the AlgR/AgrA/LytR family

2002 • 192 citations

Identification and molecular analysis of a locus that regulates extracellular toxin production in Clostridium perfringens

1994 • 188 citations

Clostridial VirR/VirS regulon involves a regulatory RNA molecule for expression of toxins

2002 • 142 citations

The virR gene, a member of a class of two-component response regulators, regulates the production of perfringolysin O, collagenase, and hemagglutinin in Clostridium perfringens

1994 • 141 citations

The virR/virS locus regulates the transcription of genes encoding extracellular toxin production in Clostridium perfringens

1996 • 140 citations

Clostridium perfringens-Escherichia coli Shuttle Vectors That Carry Single Antibiotic Resistance Determinants

1993 • 137 citations

Transferable tetracycline resistance in Clostridium difficile

1981 • 137 citations

Environmental Response and Autoregulation of Clostridium difficile TxeR, a Sigma Factor for Toxin Gene Expression

2002 • 136 citations

Efficient sporulation in Clostridium difficile requires disruption of the σ^K gene

2003 • 126 citations

Comparative analysis of Clostridium difficile clinical isolates belonging to different genetic lineages and time periods

2004 • 108 citations

The Type IV Fimbrial Subunit Gene ( fimA ) of Dichelobacter nodosus Is Essential for Virulence, Protease Secretion, and Natural Competence

2001 • 102 citations

Transferable Resistance to Clindamycin, Erythromycin, and Tetracycline in Clostridium difficile

1983 • 102 citations

Genomic analysis of the erythromycin resistance element Tn5398 from Clostridium difficile The GenBank accession number for the Tn5398 element and flanking sequence is AF109075.

2001 • 99 citations

Identification of the Putative Staphylococcal AgrB Catalytic Residues Involving the Proteolytic Cleavage of AgrD to Generate Autoinducing Peptide

2005 • 83 citations

International Typing Study of Toxin A-Negative, Toxin B-Positive Clostridium difficile Variants

2003 • 77 citations

The VirR Response Regulator from Clostridium perfringens Binds Independently to Two Imperfect Direct Repeats Located Upstream of the pfoA Promoter

2000 • 75 citations

Quorum sensing in Clostridium difficile: analysis of a luxS-type signalling system

2005 • 74 citations

The VirR/VirS regulatory cascade affects transcription of plasmid-encoded putative virulence genes inClostridium perfringensstrain 13

2003 • 69 citations

Identification of novel VirR/VirS‐regulated genes in Clostridium perfringens

2000 • 68 citations

Conjugative Transfer of RP4-oriTShuttle Vectors fromEscherichia colitoClostridium perfringens

1998 • 61 citations

Transposition of Tn4451 and Tn4453 involves a circular intermediate that forms a promoter for the large resolvase, TnpX

2000 • 58 citations

Gene Cloning in Clostridium difficile Using Tn916 as a Shuttle Conjugative Transposon

1994 • 47 citations

Clostridium beijerinckii and Clostridium difficile Detoxify Methylglyoxal by a Novel Mechanism Involving Glycerol Dehydrogenase

2001 • 46 citations

Development of an integrative vector for the expression of antisense RNA in Clostridium difficile

2003 • 41 citations

The Spatial Organization of the VirR Boxes Is Critical for VirR-Mediated Expression of the Perfringolysin O Gene, pfoA , from Clostridium perfringens

2004 • 39 citations

Detection of binary-toxin genes (cdtA and cdtB) among Clostridium difficile strains isolated from patients with C. difficile-associated diarrhoea (CDAD) in Poland

2005 • 34 citations

The FxRxHrS Motif: A Conserved Region Essential for DNA Binding of the VirR Response Regulator from Clostridium perfringens

2002 • 26 citations

Glass slide microarrays for bacterial genomes

2002 • 23 citations

Genetic Analysis of the ycgJ‐metB‐cysK‐ygaG Operon Negatively Regulated by the VirR/VirS System in Clostridium perfringens

2000 • 23 citations

Analysis of Clostridium difficile-associated diarrhea among patients hospitalized in tertiary care academic hospital

2005 • 22 citations

Microarray design for bacterial genomes

2002 • 21 citations

Gene transfer intoClostridium difficileCD630 and characterisation of its methylase genes

2003 • 17 citations

The SKHR Motif Is Required for Biological Function of the VirR Response Regulator from Clostridium perfringens

2003 • 15 citations

UK launches inquiry into Clostridium difficile outbreak

2005 • 15 citations

Quebec strikes committee on Clostridium difficile

2004 • 7 citations

The VirR Response Regulator from Clostridium perfringens Binds Independently to Two Imperfect Direct Repeats Located Upstream of the pfoA Promoter

2000 • 3 citations