Activity gel and activity blotting methods for detecting DNA-modifying (repair) enzymes

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: Results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes

1980 • 1,256 citations

[17] Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis

1983 • 1,115 citations

DNA Repair Enzymes

1982 • 905 citations

MUTAGENESIS BY APURINIC/APYRIMIDINIC SITES

1986 • 884 citations

DNA REPAIR ENZYMES

1988 • 822 citations

Nuclease detection in SDS-polyacrylamide gel electrophoresis

1977 • 291 citations

Renaturation of enzymes after polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate.

1980 • 279 citations

Activity staining of nucleolytic enzymes after sodium dodecyl sulfate-polyacrylamide gel electrophoresis: Use of aqueous isopropanol to remove detergent from gels

1982 • 206 citations

Detection of the catalytic activities of DNA polymerases and their associated exonucleases following SDS-polyacrylamide gel electrophoresis

1981 • 193 citations

Ionizing radiation-induced mutagenesis

1988 • 158 citations

[40] Locating enzymes on gels

1971 • 156 citations

Deoxyribonuclease I in mammalian tissues. Specificity of inhibition by actin.

1981 • 150 citations

A mitochondrial DNA polymerase from embryos of Drosophila melanogaster. Purification, subunit structure, and partial characterization.

1986 • 136 citations

cDNA cloning, sequencing, expression and possible domain structure of human APEX nuclease homologous to Escherichia coli exonuclease III

1992 • 122 citations

Polypeptide structure of DNA primase from a yeast DNA polymerase-primase complex.

1985 • 117 citations

cDNA and deduced amino acid sequence of a mouse DNA repair enzyme (APEX nuclease) with significant homology to Escherichia coli exonuclease III.

1991 • 109 citations

Purification and characterization of wheat germ DNA topoisomerase I (nicking-closing enzyme).

1981 • 109 citations

Purification and properties of single strand DNA-binding endo-exonuclease of Neurospora crassa.

1983 • 94 citations

Human placental DNA polymerase .delta.: identification of a 170 kilodalton polypeptide by activity staining and immunoblotting

1987 • 85 citations

Staining for enzymatic activity after gel electrophoresis, I

1992 • 83 citations

A mouse DNA repair enzyme (APEX nuclease) having exonuclease and apurinic/apyrimidinic endonuclease activities: purification and characterization

1991 • 83 citations

The mammalian primase is part of a high molecular weight DNA polymerase alpha polypeptide.

1983 • 75 citations

Characterization of the 5′ to 3′ exonuclease associated withThermus aquaticusDNA polymerase

1990 • 72 citations

Identification of deoxyribonucleases in polyacrylamide gel following their separation by disk electrophoresis

1965 • 71 citations

Detection of enzymatic activities in sodium dodecyl sulfate-polyacrylamide gels: DNA polymerases as model enzymes

1983 • 69 citations

Second Cytotoxic Pathway of Diphtheria Toxin Suggested by Nuclease Activity

1989 • 66 citations

Effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis

1979 • 64 citations

Rapid purification and characterization of DNA topoisomerase I from cultured mouse mammary carcinoma FM3A cells.

1983 • 64 citations

Genetic analysis of human deoxyribonuclease I by immunoblotting and the zymogram method following isoelectric focusing

1989 • 63 citations

Improved conditions for activity gel analysis of DNA polymerase catalytic polypeptides

1983 • 61 citations

DNA Damage and Repair during Cellular Aging

1989 • 61 citations

Detection of poly(ADP ribose) polymerase in crude extracts by activity-blot

1991 • 57 citations

Catalytic activities of human poly(ADP-ribose) polymerase from normal and mutagenized cells detected after sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

1984 • 55 citations

Proteolytic degradation of calf thymus terminal deoxynucleotidyl transferase.

1982 • 54 citations

[22] Recovery of functional proteins in sodium dodecyl sulfate gels

1983 • 53 citations

Complementation of DNA repair‐deficient Escherichia coli by the yeast Apn1 apurinic/apyrimidinic endonuclease gene

1991 • 52 citations

Striking similarity of the distribution patterns of the poly(ADP-ribose) polymerase and DNA polymerase β among various mouse organs

1990 • 47 citations

Structural analysis of poly(ADP‐ribose) polymerase in higher and lower eukaryotes

1986 • 43 citations

On the molecular weight and subunit composition of calf thymus ribonuclease H1

1990 • 43 citations

An immunochemical study of Neurospora nucleases

1986 • 40 citations

Studies on catalytic subunits of mouse myeloma alpha-polymerase.

1982 • 39 citations

An exonuclease possibly involved in the initiation of repair of bleomycin-damaged DNA in mouse ascites sarcoma cells

1988 • 39 citations

DNA polymerase-alpha. Common polypeptide core structure of three enzyme forms from human KB cells.

1982 • 38 citations

Mechanism of action of deoxyribonuclease II from human lymphoblasts

1991 • 37 citations

Multiple DNA repair activities for 3'-deoxyribose fragments inEscherichia coli

1989 • 37 citations

Depletion of adenosine diphosphate-ribosyl transferase activity in rat liver during exposure to N-2-acetylaminofluorene: effect of thiols.

1988 • 36 citations

Nuclear endo-exonuclease of Neurospora crassa. Evidence for a role in DNA repair.

1987 • 33 citations

Mammalian DNA ligase. Structure and function in rat-liver tissues

1987 • 31 citations

DNA methyltransferases: Activity minigel analysis and determination with DNA covalently bound to a solid matrix

1985 • 31 citations

A fluorometric method for the detection of endodeoxyribonuclease on DNA-polyacrylamide gels

1973 • 29 citations

Heterogeneity of mammalian DNA ligase detected on activity and DNA sequencing gets

1984 • 27 citations

Isoelectric focusing of multiple forms of DNase in thin layers of polyacrylamide gel and detection of enzymatic activity with a zymogram method following separation

1982 • 26 citations

Purification and characterization of a .gamma.-like DNA polymerase from Chlamydomonas reinhardtii

1991 • 26 citations

DNA ligase activity in human cell lines from normal donors and Bloom's syndrome patients

1989 • 23 citations

Non‐disruptive detection of DNA polymerases in nondenaturing polyacrylamide gels

1985 • 21 citations

Detection of possible DNA repair enzymes on sodium dodecyl sulfate-polyacrylamide gels by protein blotting to damaged DNA-fixed membranes

1991 • 20 citations

Thermophilic Polynucleotide Phosphorylase from Thermus thermophilus

1977 • 19 citations

Effect of polyamines on the activity of malarial α‐like DNA polymerase

1990 • 19 citations

Activity gels for analysing DNA processing enzymes

1986 • 19 citations

Repair of X-ray-induced single-strand breaks by a cell-free system

1990 • 18 citations

Partial purification and characterization of the reverse transcriptase of the simian immunodeficiency virus TYO-7 isolated from an African green monkey

1990 • 17 citations

Two-dimensional zymogram analysis of nucleases in Bacillus subtilis

1983 • 17 citations

Variation in DNA ligase structure during repair and replication processes in monkey kidney cells

1985 • 17 citations

A study by polyacrylamide-gel electrophoresis of the effect of proteolysis on Micrococcus lysodeikticus polynucleotide phosphorylase

1968 • 16 citations

Simian immunodeficiency virus reverse transcriptase

1990 • 16 citations

Isolation of Chinese hamster ovary cells with reduced poly(ADP-ribose) polymerase activity

1990 • 16 citations

Bloom's syndrome

1986 • 16 citations

A DNA polymerase with unusual properties from the slime mold Physarum polycephalum

1987 • 16 citations

Staining for enzymatic activity after gel electrophoresis

1992 • 15 citations

Properties and applications of new monoclonal antibodies raised against calf DNA polymerase α

1985 • 15 citations

Characterization of DNA metabolizing enzymes in situ following polyacrylamide gel electrophoresis

1991 • 15 citations

Purification and caracterization of DNA polymerase α from plasmodia of Physarum polycephalum

1988 • 15 citations

DNA Maintenance and its Relation to Human Pathology

1986 • 12 citations

A possible cause of heterogeneity of mammalian apurinic/apyrimidinic endonuclease

1993 • 11 citations

Characterization of Messenger RNA for Chick‐Embryo DNA Polymerase β and Its Translation Product in vitro

1983 • 10 citations

Purification and some properties of a thermostable DNA polymerase from a Thermotoga species

1990 • 10 citations

Enhanced Deoxyribonuclease Activity in Human Transformed Cells and in Bloom's Syndrome Cells

1989 • 10 citations

An animal virus-induced DNA methyltransferase

1988 • 9 citations

A single, phosphate-repressible deoxyribonuclease, DNase A, secreted inAspergillus nidulans

1989 • 9 citations

DNA polymerase alpha-primase complexes from carcinogen-treated Chinese hamster ovary cells.

1990 • 8 citations

Purification and characterization of a DNA polymerase from the cyanobacteriumAnacystis nidulansR2

1990 • 7 citations

Use of methyl green-DNA agarose for detecting deoxyribonuclease activity in polyacrylamide gels

1981 • 7 citations

Assays and structure of reverse transcriptase of the AIDS virus

1989 • 6 citations

Deoxyribonuclease zymography adapted to the precast PhastGel electrophoresis media

1990 • 5 citations

Purification and Activity Gel Analysis of Polynucleotide Phosphorylase from the Cyanobacterium Nostoc sp. MAC

1989 • 4 citations

[Determination of DNA nonradioactively labeled with photobiotin using streptavidin conjugates with peroxidase or alkaline phosphatase].

1988 • 3 citations

A single, phosphate-repressible deoxyribonuclease, DNase A, secreted inAspergillus nidulans

1989 • 1 citations