Staining for enzymatic activity after gel electrophoresis

Enzymatic Synthesis of Deoxyribonucleic Acid

1969 • 543 citations

Nuclease detection in SDS-polyacrylamide gel electrophoresis

1977 • 291 citations

Renaturation of enzymes after polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate.

1980 • 279 citations

Activity staining of nucleolytic enzymes after sodium dodecyl sulfate-polyacrylamide gel electrophoresis: Use of aqueous isopropanol to remove detergent from gels

1982 • 206 citations

Detection of the catalytic activities of DNA polymerases and their associated exonucleases following SDS-polyacrylamide gel electrophoresis

1981 • 193 citations

DNA sequence of the gene coding for Escherichia coli ribonuclease H.

1983 • 167 citations

A mitochondrial DNA polymerase from embryos of Drosophila melanogaster. Purification, subunit structure, and partial characterization.

1986 • 136 citations

Polypeptide structure of DNA primase from a yeast DNA polymerase-primase complex.

1985 • 117 citations

Purification and properties of single strand DNA-binding endo-exonuclease of Neurospora crassa.

1983 • 94 citations

Human placental DNA polymerase .delta.: identification of a 170 kilodalton polypeptide by activity staining and immunoblotting

1987 • 85 citations

The mammalian primase is part of a high molecular weight DNA polymerase alpha polypeptide.

1983 • 75 citations

Characterization of the 5′ to 3′ exonuclease associated withThermus aquaticusDNA polymerase

1990 • 72 citations

Identification of deoxyribonucleases in polyacrylamide gel following their separation by disk electrophoresis

1965 • 71 citations

Detection of enzymatic activities in sodium dodecyl sulfate-polyacrylamide gels: DNA polymerases as model enzymes

1983 • 69 citations

Second Cytotoxic Pathway of Diphtheria Toxin Suggested by Nuclease Activity

1989 • 66 citations

Genetic analysis of human deoxyribonuclease I by immunoblotting and the zymogram method following isoelectric focusing

1989 • 63 citations

Improved conditions for activity gel analysis of DNA polymerase catalytic polypeptides

1983 • 61 citations

Proteolytic degradation of calf thymus terminal deoxynucleotidyl transferase.

1982 • 54 citations

Comparison of nucleic acid-protein interactions in solution and in polyacrylamide gels

1986 • 52 citations

Blue‐Dextran — Sepharose Affinity Chromatography: Recognition of a Polynucleotide Binding Site of a Protein

1978 • 50 citations

Striking similarity of the distribution patterns of the poly(ADP-ribose) polymerase and DNA polymerase β among various mouse organs

1990 • 47 citations

On the molecular weight and subunit composition of calf thymus ribonuclease H1

1990 • 43 citations

Structural analysis of poly(ADP‐ribose) polymerase in higher and lower eukaryotes

1986 • 43 citations

A general method for the localization of enzymes that produce phosphate, pyrophosphate, or CO2 after polyacrylamide gel electrophoresis

1982 • 43 citations

Cloning of E. coli pnp gene from an episome

1981 • 41 citations

An immunochemical study of Neurospora nucleases

1986 • 40 citations

Structural homology among calf thymus α-polymerase polypeptides

1982 • 40 citations

Studies on catalytic subunits of mouse myeloma alpha-polymerase.

1982 • 39 citations

DNA polymerase-alpha. Common polypeptide core structure of three enzyme forms from human KB cells.

1982 • 38 citations

Antibody-linked polymerase assay on protein blots: a novel method for identifying polymerases following SDS-polyacrylamide gel electrophoresis.

1983 • 36 citations

Depletion of adenosine diphosphate-ribosyl transferase activity in rat liver during exposure to N-2-acetylaminofluorene: effect of thiols.

1988 • 36 citations

Changes in activity and mRNA levels of poly(ADP-ribose) polymerase during rat liver regeneration

1990 • 33 citations

Detection of nucleases degrading double helical RNA and of nucleic acid‐binding proteins following SDS‐gel electrophoresis

1978 • 32 citations

Mammalian DNA ligase. Structure and function in rat-liver tissues

1987 • 31 citations

DNA methyltransferases: Activity minigel analysis and determination with DNA covalently bound to a solid matrix

1985 • 31 citations

A fluorometric method for the detection of endodeoxyribonuclease on DNA-polyacrylamide gels

1973 • 29 citations

Heterogeneity of mammalian DNA ligase detected on activity and DNA sequencing gets

1984 • 27 citations

Isoelectric focusing of multiple forms of DNase in thin layers of polyacrylamide gel and detection of enzymatic activity with a zymogram method following separation

1982 • 26 citations

Purification and characterization of a .gamma.-like DNA polymerase from Chlamydomonas reinhardtii

1991 • 26 citations

Structure et activité de la polynucléotide phosphorylase d'Escherichia coli: une espèce à faible poids moléculaire

1969 • 26 citations

Nachweis pflanzlicher Ribonukleasen in Polyacrylamid-Gelen nach Disk-Elektrophorese

1968 • 26 citations

DNA ligase activity in human cell lines from normal donors and Bloom's syndrome patients

1989 • 23 citations

Purification and Properties of an Alkaline Ribonuclease from the Hepatic Cytosol Fraction of Bullfrog, Rana catesbeiana

1976 • 22 citations

Polynucleotide‐acrylamide gel electrophoresis of soluble nucleases from tobacco leaves

1975 • 21 citations

Non‐disruptive detection of DNA polymerases in nondenaturing polyacrylamide gels

1985 • 21 citations

Human serum deoxyribonuclease assay in [3H]DNA-polyacrylamide gels without staining artifacts

1974 • 20 citations

Detection of possible DNA repair enzymes on sodium dodecyl sulfate-polyacrylamide gels by protein blotting to damaged DNA-fixed membranes

1991 • 20 citations

Thermophilic Polynucleotide Phosphorylase from Thermus thermophilus

1977 • 19 citations

Effect of polyamines on the activity of malarial α‐like DNA polymerase

1990 • 19 citations

Properties of a Cytoplasmic Proteolytic Enzyme from Escherichia coli

1975 • 19 citations

Characterization of polynucleotide phosphorylase from Micrococcus luteus and isolation of the 13,000 base poly(A) product of the polymerization reaction.

1982 • 18 citations

The occurrence of two ribosomal ribonucleases depending on growth phase in yeast

1981 • 17 citations

Variation in DNA ligase structure during repair and replication processes in monkey kidney cells

1985 • 17 citations

Partial purification and characterization of the reverse transcriptase of the simian immunodeficiency virus TYO-7 isolated from an African green monkey

1990 • 17 citations

Study on the structure-function relationship of polynucleotide phosphorylase: model of a proteolytic degraded polynucleotide phosphorylase

1976 • 17 citations

Two-dimensional zymogram analysis of nucleases in Bacillus subtilis

1983 • 17 citations

A DNA polymerase with unusual properties from the slime mold Physarum polycephalum

1987 • 16 citations

Simian immunodeficiency virus reverse transcriptase

1990 • 16 citations

Bloom's syndrome

1986 • 16 citations

A study by polyacrylamide-gel electrophoresis of the effect of proteolysis on Micrococcus lysodeikticus polynucleotide phosphorylase

1968 • 16 citations

Characterization of DNA metabolizing enzymes in situ following polyacrylamide gel electrophoresis

1991 • 15 citations

Purification and caracterization of DNA polymerase α from plasmodia of Physarum polycephalum

1988 • 15 citations

Properties and applications of new monoclonal antibodies raised against calf DNA polymerase α

1985 • 15 citations

Synthesis of DNA polymerase by in vitro translation of calf RNA

1984 • 12 citations

Detection of DNA and RNA polymerase activities in situ following electrophoresis in polyacrylamide gels

1976 • 11 citations

Purification and some properties of a thermostable DNA polymerase from a Thermotoga species

1990 • 10 citations

Enhanced Deoxyribonuclease Activity in Human Transformed Cells and in Bloom's Syndrome Cells

1989 • 10 citations

Processive and Synchronous Mechanism of Polynucleotide Phosphorylase Phosphorolysis

1977 • 10 citations

Electrophoresis of acid phosphohydrolase isozymes on cellogel

1977 • 10 citations

An animal virus-induced DNA methyltransferase

1988 • 9 citations

A single, phosphate-repressible deoxyribonuclease, DNase A, secreted inAspergillus nidulans

1989 • 9 citations

Changes in ribonuclease activity during development of the mosquito, Aedes aegypti

1986 • 8 citations

DNA polymerase alpha-primase complexes from carcinogen-treated Chinese hamster ovary cells.

1990 • 8 citations

Purification and Properties of Two Ribonucoleases in Different Intracellular Compartments in Pea Root Tissue

1975 • 8 citations

Evidence for multiple ribonucleases in crude extracts from cultured mosquito cells

1985 • 8 citations

Use of methyl green-DNA agarose for detecting deoxyribonuclease activity in polyacrylamide gels

1981 • 7 citations

A positive zymogram method for ribonuclease

1979 • 7 citations

Purification and characterization of a DNA polymerase from the cyanobacteriumAnacystis nidulansR2

1990 • 7 citations

The origin of the electrophoretic microheterogeneity in primer-independent polynucleotide phosphorylase from Micrococcus luteus.

1978 • 6 citations

Electrophoretic and zymogram analysis of the cytoplasmic soluble protein fraction of Spiroplasma citri

1988 • 6 citations

Assays and structure of reverse transcriptase of the AIDS virus

1989 • 6 citations

Deoxyribonuclease zymography adapted to the precast PhastGel electrophoresis media

1990 • 5 citations

Cloning and expression of a cDNA encoding a catalytically active fragment of calf thymus DNA polymerase alpha

1986 • 5 citations

Partial purification and some properties of a liver alkaline ribonuclease from the frog Rana esculenta.

1979 • 5 citations

Improved method for ribonuclease zymogram

1981 • 5 citations

Purification and Activity Gel Analysis of Polynucleotide Phosphorylase from the Cyanobacterium Nostoc sp. MAC

1989 • 4 citations

[Determination of DNA nonradioactively labeled with photobiotin using streptavidin conjugates with peroxidase or alkaline phosphatase].

1988 • 3 citations