Functional Immunoliposomes Harboring a Biosynthetically Lipid-Tagged Single-Chain Antibody

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

Production of large unilamellar vesicles by a rapid extrusion procedure. Characterization of size distribution, trapped volume and ability to maintain a membrane potential

1985 • 2,037 citations

Preparation of liposomes of defined size distribution by extrusion through polycarbonate membranes

1979 • 1,085 citations

Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent

1988 • 1,030 citations

Antibody fragments from a ‘single pot’ phage display library as immunochemical reagents.

1994 • 578 citations

Immobilized metal affinity adsorption and immobilized metal affinity chromatography of biomaterials. Serum protein affinities for gel-immobilized iron and nickel ions

1983 • 508 citations

Bioanalysis with surface plasmon resonance

1991 • 341 citations

Miniantibodies: use of amphipathic helixes to produce functional, flexibly linked dimeric FV fragments with high avidity in Escherichia coli

1992 • 294 citations

Homing of liposomes to target cells

1975 • 291 citations

The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment

1993 • 288 citations

Integrated fluid handling system for biomolecular interaction analysis

1991 • 263 citations

The Functional Expression of Antibody Fv Fragments in Ischhuchia coli: Improved Vectors and a Generally Applicable Purification Technique

1991 • 243 citations

Antibody-targeted delivery of doxorubicin entrapped in sterically stabilized liposomes can eradicate lung cancer in mice.

1993 • 239 citations

pH-sensitive immunoliposomes mediate target-cell-specific delivery and controlled expression of a foreign gene in mouse.

1987 • 235 citations

Immunospecific targeting of liposomes to cells: a novel and efficient method for covalent attachment of Fab' fragments via disulfide bonds

1981 • 214 citations

Monoclonal antibody covalently coupled with fatty acid. A reagent for in vitro liposome targeting.

1980 • 190 citations

Lipid composition is important for highly efficient target binding and retention of immunoliposomes.

1990 • 174 citations

Efficacy and Upper GI Safety of Diclofenac/Misoprostol, Piroxicam and Naproxen in Patients with Osteoarthritis

1993 • 167 citations

Characterization of new membrane lipoproteins and their precursors of Escherichia coli.

1981 • 146 citations

Building Antibodies from their Genes

1992 • 143 citations

Mono‐ and Bivalent Antibody Fragments Produced in Escherichia coli: Engineering, Folding and Antigen Binding

1992 • 133 citations

Bacterial expression of immunoglobulin fragments

1993 • 132 citations

Construction, Bacterial Expression and Characterization of a Bifunctional Single–Chain Antibody–Phosphatase Fusion Protein Targeted to the Human ERBB–2 Receptor

1992 • 120 citations

Improved Bivalent Miniantibodies, with Identical Avidity as Whole Antibodies, Produced by High Cell Density Fermentation of Escherichia coli

1993 • 116 citations

Inheritance of antibody specificity V. Anti-2-phenyloxazolone in the mouse.

1978 • 116 citations

Antibody-targeted liposomes: increase in specific toxicity of methotrexate-gamma-aspartate.

1983 • 111 citations

An active single-chain antibody containing a cellulase linker domain is secreted by Escherichia coli

1991 • 107 citations

A bifunctional fusion protein containing Fc-binding fragment B of staphylococcal protein A amino-terminal to antidigoxin single-chain Fv

1990 • 104 citations

The protein sequence responsible for lipoprotein membrane localization in Escherichia coli exhibits remarkable specificity.

1991 • 96 citations

Nine amino acid residues at the NH2-terminal of lipoprotein are sufficient for its modification, processing, and localization in the outer membrane of Escherichia coli.

1984 • 87 citations

Monoclonal antibody targeting of liposomes to mouse lung in vivo.

1989 • 79 citations

Recent advances in phage display

1993 • 65 citations

Trimeric structure and localization of the major lipoprotein in the cell surface of Escherichia coli.

1986 • 62 citations

Recombinant Colorimetric Antibodies: Construction and Characterization of a Bifunctional F(ab)2/Alkaline Phosphatase Conjugate Produced in Escherichia coli

1993 • 57 citations

[22] Sensitive protein assay in presence of high levels of lipid

1989 • 55 citations

Generic liposome reagent for immunoassays

1989 • 47 citations

Lipid-tagged antibodies: bacterial expression and characterization of a lipoprotein—single-chain antibody fusion protein

1993 • 44 citations

Stable target-sensitive immunoliposomes

1992 • 38 citations

Two-dimensional recognition pattern of lipid-anchored Fab' fragments

1990 • 35 citations

Targeting of monoclonal antibody-coated liposomes to sheep red blood cells

1981 • 27 citations

Antibody-bearing liposomes improve agglutination of latex particles used in clinical diagnostic assays

1985 • 21 citations

Biosynthetic lipid‐tagging of antibodies

1994 • 19 citations

Stable liposomes for assays of human sera

1993 • 19 citations

Recombinant antibody-metallothionein: design and evaluation for radioimmunoimaging.

1992 • 13 citations

Antigen Responsive Antibody–Receptor Kinase Chimera

1992 • 12 citations

The use of streptavidin-biotin interaction for preparation of reagents for complement-dependent liposome immunoassay of proteins: Detection of latrotoxin

1992 • 8 citations

Aggregation of hapten-bearing liposomes mediated by specific antibodies

1993 • 7 citations