Techniques: Recombinogenic engineering–new options for cloning and manipulating DNA

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products

2000 • 14,118 citations

Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter

1995 • 4,806 citations

A simplified system for generating recombinant adenoviruses

1998 • 3,179 citations

An efficient recombination system for chromosome engineering in Escherichia coli

2000 • 1,679 citations

Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.

1992 • 1,651 citations

Yeast transformation: a model system for the study of recombination.

1981 • 1,491 citations

A new logic for DNA engineering using recombination in Escherichia coli

1998 • 1,199 citations

Biochemistry of homologous recombination in Escherichia coli

1994 • 1,195 citations

Recombinational Repair of DNA Damage in Escherichia coli and Bacteriophage λ

1999 • 902 citations

A new bacteriophage P1–derived vector for the propagation of large human DNA fragments

1994 • 857 citations

Genetic engineering. Principles and methods

1987 • 715 citations

New method for generating deletions and gene replacements in Escherichia coli

1989 • 680 citations

Efficient generation of recombinant adenovirus vectors by homologous recombination in Escherichia coli

1996 • 655 citations

Use of Bacteriophage λ Recombination Functions To Promote Gene Replacement in Escherichia coli

1998 • 552 citations

Homologous recombination based modification in Esherichia coli and germline transmission in transgenic mice of a bacterial artificial chromsome

1997 • 551 citations

Rapid modification of bacterial artificial chromosomes by ET- recombination

1999 • 533 citations

Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome

1997 • 419 citations

DNA cloning by homologous recombination in Escherichia coli

2000 • 415 citations

Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli

1985 • 342 citations

Cloning and Mutagenesis of the Murine Gammaherpesvirus 68 Genome as an Infectious Bacterial Artificial Chromosome

2000 • 328 citations

PCR-mediated gene replacement in Escherichia coli

2000 • 287 citations

Reduction of liver Fas expression by an antisense oligonucleotide protects mice from fulminant hepatitis

2000 • 275 citations

Construction of Large DNA Segments in Escherichia coli

1989 • 194 citations

RecE/RecT and Redα/Redβ initiate double-stranded break repair by specifically interacting with their respective partners

2000 • 185 citations

Rapid engineering of bacterial artificial chromosomes using oligonucleotides

2000 • 158 citations

Lambda Gam protein inhibits the helicase and chi-stimulated recombination activities of Escherichia coli RecBCD enzyme

1991 • 153 citations

Selective isolation of cosmid clones by homologous recombination in Escherichia coli.

1984 • 151 citations

Efficient and precise engineering of a 200 kb β-globin human/bacterial artificial chromosome in E. coli DH10B using an inducible homologous recombination system

1999 • 143 citations

Point mutation of bacterial artificial chromosomes by ET recombination

2000 • 137 citations

Modification of bacterial artificial chromosomes through Chi-stimulated homologous recombination and its application in zebrafish transgenesis

1998 • 137 citations

Forward with BACs

2000 • 119 citations

Homologous pairing proteins encoded by the Escherichia coii recE and recT genes

1994 • 91 citations

Cloning high molecular weight DNA fragments by the bacteriophage P1 system

1992 • 78 citations

Simplified generation of targeting constructs using ET recombination

1999 • 73 citations

Simplified generation of targetting constructs using ET recombination

1999 • 71 citations

Modification of human beta-globin locus PAC clones by homologous recombination in Escherichia coli

2000 • 55 citations

Insertion of disease-causing mutations in BACs by homologous recombination in Escherichia coli

2000 • 49 citations

Rapid Construction in Yeast of Complex Targeting Vectors for Gene Manipulation in the Mouse

1996 • 48 citations

Gene Replacement With Linear DNA Fragments in Wild-Type Escherichia coli: Enhancement by Chi Sites

1997 • 48 citations

Recombinogenic targeting: a new approach to genomic analysis—a review

1998 • 35 citations

ET-Cloning: Think Recombination First

2000 • 35 citations

In vivo intermolecular recombination in Escherichia coli: application to plasmid constructions

1996 • 29 citations

Direct Cloning of Genomic DNA by Recombinogenic Targeting Method Using a Yeast–Bacterial Shuttle Vector, pClasper

1999 • 22 citations

BAC Trimming: Minimizing Clone Overlaps

2000 • 19 citations

Manipulating large genomic clones via in vivo recombination in bacteria

1999 • 9 citations