One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products

Studies on transformation of Escherichia coli with plasmids

1983 • 11,179 citations

The Complete Genome Sequence of Escherichia coli K-12

1997 • 7,325 citations

Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter

1995 • 4,806 citations

Escherichia Coli and Salmonella: Typhimurium Cellular and Molecular Biology

1987 • 4,385 citations

Escherichia coli and Salmonella typhimurium: Cellular and molecular biology

1988 • 3,957 citations

Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant

1995 • 1,866 citations

A simple and efficient method for direct gene deletion inSaccharomyces cerevisiae

1993 • 1,256 citations

Bacterial gene transfer by natural genetic transformation in the environment

1994 • 1,253 citations

Systematic functional analysis of the yeast genome

1998 • 1,213 citations

A new logic for DNA engineering using recombination in Escherichia coli

1998 • 1,199 citations

Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization

1997 • 858 citations

Rapid Hypothesis Testing with Candida albicans through Gene Disruption with Short Homology Regions

1999 • 765 citations

New method for generating deletions and gene replacements in Escherichia coli

1989 • 680 citations

Use of Bacteriophage λ Recombination Functions To Promote Gene Replacement in Escherichia coli

1998 • 552 citations

Conditionally Replicative and Conjugative Plasmids CarryinglacZα for Cloning, Mutagenesis, and Allele Replacement in Bacteria

1996 • 431 citations

Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli

1985 • 342 citations

Methods in molecular genetics

1995 • 319 citations

recD: the gene for an essential third subunit of exonuclease V.

1986 • 276 citations

Homologous recombination in procaryotes

1988 • 271 citations

Markerless gene replacement in Escherichia coli stimulated by a double-strand break in the chromosome

1999 • 254 citations

Identification and characterization of recD, a gene affecting plasmid maintenance and recombination in Escherichia coli

1986 • 219 citations

Homologous Genetic Recombination: The Pieces Begin to Fall into Place

1994 • 199 citations

Specific-purpose plasmid cloning vectors I. Low copy number, temperature-sensitive, mobilization-defective pSC101-derived containment vectors

1981 • 198 citations

Chromosomal transformation of Escherichia coli recD strains with linearized plasmids

1989 • 167 citations

Genetic Transformation in Escherichia coli K12

1973 • 143 citations

Use of New Methods for Construction of Tightly Regulated Arabinose and Rhamnose Promoter Fusions in Studies of the Escherichia coli Phosphate Regulon

1998 • 131 citations

MUTANTS AFFECTED IN ALKALINE PHOSPHATASE EXPRESSION: EVIDENCE FOR MULTIPLE POSITIVE REGULATORS OF THE PHOSPHATE REGULON IN ESCHERICHIA COLI

1980 • 121 citations

Molecular cloning, mapping, and regulation of Pho regulon genes for phosphonate breakdown by the phosphonatase pathway of Salmonella typhimurium LT2

1995 • 106 citations

Identification of phosphate starvation-inducible genes in Escherichia coli K-12 by DNA sequence analysis of psi::lacZ(Mu d1) transcriptional fusions

1990 • 101 citations

Sequence identity of then-1 product of a synthetic oligonucleotide

1995 • 94 citations

The nucleotide sequence recognized by the Escherichia coli K12 restriction and modification enzymes

1979 • 93 citations

In vivoexcision and amplification of large segments of theEscherichia coligenome

1994 • 71 citations

Overlapping and separate controls on the phosphate regulon in Escherichia coli K12

1983 • 68 citations

Transcriptional regulation of the Enterococcus faecium BM4147 vancomycin resistance gene cluster by the VanS-VanR two-component regulatory system in Escherichia coli K-12

1997 • 66 citations

Mutations determining generalized resistance to aminoglycoside antibiotics in Escherichia coli

1978 • 57 citations

Escherichia coli genome targeting I. Cre-Zox-mediated in vitro generation of ori− plasmids and their in vivo chromosomal integration and retrieval

1994 • 54 citations

Gene Replacement With Linear DNA Fragments in Wild-Type Escherichia coli: Enhancement by Chi Sites

1997 • 48 citations

Analysis of internal (n-1)mer deletion sequences in synthetic oligodeoxyribonucleotides by hybridization to an immobilized probe array

1999 • 46 citations

Locus determining normal colony formation on the chromosome of Escherichia coli K-12

1967 • 26 citations

A Rapid Method for Disrupting Genes in theEscherichia coliGenome

1998 • 12 citations