Recombinant DNA procedures for producing small antimicrobial cationic peptides in bacteria

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2001 • 133,517 citations

Current protocols in molecular biology

1988 • 7,584 citations

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1988 • 6,107 citations

A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

1985 • 3,185 citations

High resolution acrylamide gel electrophoresis of histones

1969 • 2,470 citations

Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of two active forms, and partial cDNA sequence of a precursor.

1987 • 2,216 citations

The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage

1983 • 1,315 citations

Insect Immunity. Purification and Properties of Three Inducible Bactericidal Proteins from Hemolymph of Immunized Pupae of Hyalophora cecropia

1980 • 960 citations

A synthetic IgG-binding domain based on staphylococcal protein A

1987 • 735 citations

Interaction of human defensins with Escherichia coli. Mechanism of bactericidal activity.

1989 • 695 citations

All-D amino acid-containing channel-forming antibiotic peptides.

1990 • 656 citations

Primary structures of three human neutrophil defensins.

1985 • 529 citations

Insect Immunity: Isolation and Structure of Cecropin D and Four Minor Antibacterial Components from Cecropia Pupae

1982 • 427 citations

Outer membrane of Pseudomonas aeruginosa: heat- 2-mercaptoethanol-modifiable proteins

1979 • 426 citations

[Sequence analysis of melittin from tryptic and peptic degradation products].

1967 • 381 citations

Sequenzanalyse des Melittins aus den tryptischen und peptischen Spaltstücken

1967 • 332 citations

Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors.

1985 • 331 citations

Shortened cecropin A‐melittin hybrids Significant size reduction retains potent antibiotic activity

1992 • 262 citations

Outer membrane permeability in Pseudomonas aeruginosa: comparison of a wild-type with an antibiotic-supersusceptible mutant

1982 • 256 citations

Defensins

1990 • 241 citations

Engineering proteins for purification

1990 • 165 citations

Determination of the disulfide array in the human defensin HNP-2

1989 • 156 citations

Interaction of macrophage cationic proteins with the outer membrane of Pseudomonas aeruginosa

1988 • 148 citations

Isolation and characterization of human defensin cDNA clones.

1988 • 145 citations

Nucleotide sequence of the alkaline phosphatase gene of Escherichia coli

1986 • 140 citations

Augmentation of the antibacterial activity of magainin by positive-charge chain extension

1992 • 131 citations

A gene fusion system for generating antibodies against short peptides

1987 • 123 citations

Indirect Method for Assessing the Penetration of Beta-Lactamase-Nonsusceptible Penicillins and Cephalosporins in Escherichia coli Strains

1976 • 109 citations

Magainins, a class of antimicrobial peptides from Xenopus skin: Isolation, characterization of two active forms, and partial cDNA sequence of a precursor

1988 • 72 citations

Biologically active and amidated cecropin produced in a baculovirus expression system from a fusion construct containing the antibody-binding part of protein A

1991 • 52 citations

Analysis of the Pseudomonas aeruginosa major outer membrane protein OprF by use of truncated OprF derivatives and monoclonal antibodies

1992 • 40 citations

Expression of a gene encoding a scorpion insectotoxin peptide in yeast, bacteria and plants

1992 • 37 citations

Role of host factors in the regulation of the enterotoxin B gene

1991 • 37 citations

Expression and post‐translational processing of preprocecropin A using a baculovirus vector

1991 • 32 citations

Expression and secretion in yeast of active insect defensin, an inducible antibacterial peptide from the fleshflyPhormia terranovae

1992 • 28 citations