High-Level Expression of a Recombinant Antibody from Myeloma Cells Using a Glutamine Synthetase Gene as an Amplifiable Selectable Marker

A New Mouse Myeloma Cell Line that Has Lost Immunoglobulin Expression but Permits the Construction of Antibody-Secreting Hybrid Cell Lines

1979 • 1,885 citations

[1] Preparation of monoclonal antibodies: Strategies and procedures

1981 • 1,787 citations

A better cell line for making hybridomas secreting specific antibodies

1978 • 1,649 citations

Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line.

1982 • 923 citations

GENE AMPLIFICATION

1984 • 580 citations

THE GROWTH RESPONSE OF MAMMALIAN CELLS IN TISSUE CULTURE TO l-GLUTAMINE AND l-GLUTAMIC ACID

1956 • 349 citations

Assembly and secretion of heavy chains that do not associate posttranslationally with immunoglobulin heavy chain-binding protein.

1987 • 343 citations

Transcriptional interference and termination between duplicated α-globin gene constructs suggests a novel mechanism for gene regulation

1986 • 312 citations

Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors.

1981 • 303 citations

High Level Expression of Tissue Inhibitor of Metalloproteinases in Chinese Hamster Ovary Cells Using Glutamine Synthetase Gene Amplification

1990 • 249 citations

Glutamine: Metabolism, Enzymology, and Regulation

1980 • 220 citations

Transfectomas Provide Novel Chimeric Antibodies

1985 • 184 citations

High-level expression of chimeric antibodies using adapted cDNA variable region cassettes

1989 • 158 citations

Production of a mouse-human chimeric monoclonal antibody to CD20 with potent Fc-dependent biologic activity.

1987 • 143 citations

High Level Expression of the Humanized Monoclonal Antibody CAMPATH-1H in Chinese Hamster Ovary Cells

1991 • 142 citations

Synthesis of calf prochymosin (prorennin) in Escherichia coli.

1983 • 141 citations

Chimeric antibody with human constant regions and mouse variable regions directed against carcinoma-associated antigen 17-1A.

1987 • 137 citations

Immunoglobulin chain loss in hybridoma lines.

1980 • 129 citations

The inhibition by glutamine of glutamyl transferase formation in cultures of human cells

1958 • 125 citations

Effects of the position of the simian virus 40 enhancer on expression of multiple transcription units in a single plasmid.

1986 • 118 citations

A genetically engineered murine/human chimeric antibody retains specificity for human tumor-associated antigen.

1990 • 111 citations

Cloning of the genes for T84.66, an antibody that has a high specificity and affinity for carcinoembryonic antigen, and expression of chimeric human/mouse T84.66 genes in myeloma and Chinese hamster ovary cells.

1990 • 101 citations

Expression in COS cells of a mouse—human chimaeric B72.3 antibody

1987 • 100 citations

Effects of methotrexate on transfected DNA stability in mammalian cells.

1990 • 97 citations

The cloning and nucleotide sequence of cDNA for an amplified glutamine synthetase gene from the Chinese hamster

1986 • 94 citations

A genetically engineered murine/human chimeric antibody retains specificity for human tumor-associated antigen.

1986 • 86 citations

Amplified expression constructs for human tissue-type plasminogen activator in Chinese hamster ovary cells: instability in the absence of selective pressure

1988 • 81 citations

Recombinant human-mouse chimeric monoclonal antibody specific for common acute lymphocytic leukemia antigen.

1987 • 80 citations

Amplification and cloning of the Chinese hamster glutamine synthetase gene.

1984 • 78 citations

Expression of antibody genes in nonlymphoid mammalian cells

1991 • 74 citations

Expression, purification and characterization of a mouse-human chimeric antibody and chimeric Fab' fragment

1992 • 73 citations

Mouse glutamine synthetase is encoded by a single gene that can be expressed in a localized fashion

1989 • 70 citations

The use of engineered E1A genes to transactivate the hCMV-MIE promoter in permanent CHO cell lines

1991 • 68 citations

Cloning and high level expression of a chimeric antibody with specificity for human carcinoembryonic antigen.

1988 • 62 citations

High level synthesis of immunoglobulins in Chinese hamster ovary cells.

1990 • 59 citations

Application of flow cytometric measurement of surface IgG in kinetic analysis of monoclonal antibody synthesis and secretion by murine hybridoma cells

1989 • 58 citations

Enhanced transcription of the 78,000-dalton glucose-regulated protein (GRP78) gene and association of GRP78 with immunoglobulin light chains in a nonsecreting B-cell myeloma line (NS-1).

1989 • 54 citations

Glutamine regulates glutamine synthetase expression in skeletal muscle cells in culture

1990 • 51 citations

Mechanism of d-glutamyltransferase repression in mammalian cells

1963 • 47 citations

The effect of dihydrofolate reductase-mediated gene amplification on the expression of transfected immunoglobulin genes.

1987 • 40 citations

Expression of Human Anti-Tetanus Toxoid Antibody in Transfected Murine Myeloma Cells

1989 • 39 citations

An improved heat‐stable glutamine‐free chemically defined medium for growth of mammalian cells

1971 • 32 citations

The effects of adapting human diploid cells to grow in glutamic acid media on cell morphology, growth and metabolism

1973 • 26 citations

Mechanism of d-glutamyltransferase repression in mammalian cells

1963 • 15 citations

Production of recombinant proteins in serum-free media

1991 • 14 citations

GENETIC ENGINEERING OF CELLULAR PHYSIOLOGY

1991 • 4 citations