The regulation of the binding affinity of the luteinizing hormone/choriogonadotropin receptor by sodium ions is mediated by a highly conserved aspartate located in the second transmembrane domain of G protein-coupled receptors.

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

LIGAND: A versatile computerized approach for characterization of ligand-binding systems

1980 • 8,534 citations

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

High-efficiency transformation of mammalian cells by plasmid DNA.

1987 • 5,377 citations

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

1989 • 3,067 citations

Simultaneous analysis of families of sigmoidal curves: application to bioassay, radioligand assay, and physiological dose-response curves.

1978 • 2,400 citations

Lutropin-Choriogonadotropin Receptor: An Unusual Member of the G Protein-Coupled Receptor Family

1989 • 955 citations

Sequence Alignment of the G-Protein Coupled Receptor Superfamily

1992 • 847 citations

The Testicular Receptor for Follicle Stimulating Hormone: Structure and Functional Expression of Cloned cDNA

1990 • 504 citations

Cloning and functional expression of a mouse gonadotropin-releasing hormone receptor.

1992 • 357 citations

An aspartate conserved among G-protein receptors confers allosteric regulation of alpha 2-adrenergic receptors by sodium.

1990 • 266 citations

Cloning, chromosomal assignment, and regulation of the rat thyrotropin receptor: expression of the gene is regulated by thyrotropin, agents that increase cAMP levels, and thyroid autoantibodies.

1990 • 225 citations

Intronic nature of the rat luteinizing hormone receptor gene defines a soluble receptor subspecies with hormone binding activity.

1990 • 218 citations

Characteristics of a solubilized thyrotropin receptor from bovine thyroid plasma membranes

1975 • 134 citations

Human choriogonadotropin binds to a lutropin receptor with essentially no N-terminal extension and stimulates cAMP synthesis

1991 • 100 citations

The Inositol Phosphate/Diacylglycerol Pathway in MA-10 Leydig Tumor Cells

1989 • 82 citations

The influence of Na+ on the alpha 2-adrenergic receptor system of human platelets. A method for removal of extraplatelet Na+. Effect of Na+ removal on aggregation, secretion, and cAMP accumulation.

1983 • 74 citations

Specific antibody to the thyrotropin receptor identifies multiple receptor forms in membranes of cells transfected with wild-type receptor complementary deoxyribonucleic acid: characterization of their relevance to receptor synthesis, processing, structure, and function.

1992 • 62 citations

Identification of hormone-binding regions of the luteinizing hormone/human chorionic gonadotropin receptor using synthetic peptides.

1992 • 62 citations

On the Fates of Receptor-Bound Ovine Luteinizing Hormone and Human Chorionic Gonadotropin in Cultured Leydig Tumor Cells. Demonstration of Similar Rates of Internalization*

1987 • 43 citations

Na+ modulates the affinity of the lutropin/choriogonadotropin receptor.

1984 • 40 citations

Functional states of the luteinizing hormone/choriogonadotropin-receptor complex in rat Leydig cells.

1986 • 31 citations

Signal Transduction by the Human Thyrotropin Receptor: Studies on the Role of Individual Amino Acid Residues in the Carboxyl Terminal Region of the Third Cytoplasmic Loop

1991 • 22 citations