Identification of Regions in HIV-1 Nef Required for Efficient Downregulation of Cell Surface CD4

Importance of the nef gene for maintenance of high virus loads and for development of AIDS

1991 • 1,613 citations

Serine phosphorylation-independent downregulation of cell-surface CD4 by nef

1991 • 760 citations

Trans-activation of human immunodeficiency virus occurs via a bimodal mechanism

1986 • 730 citations

Nef induces CD4 endocytosis: Requirement for a critical dileucine motif in the membrane-proximal CD4 cytoplasmic domain

1994 • 701 citations

Proline-rich (PxxP) motifs in HIV-1 Nef bind to SH3 domains of a subset of Src kinases and are required for the enhanced growth of Nef+ viruses but not for down-regulation of CD4.

1995 • 557 citations

The human immunodeficiency virus-1 nef gene product: a positive factor for viral infection and replication in primary lymphocytes and macrophages.

1994 • 536 citations

Crystal Structure of the Conserved Core of HIV-1 Nef Complexed with a Src Family SH3 Domain

1996 • 433 citations

The importance of nef in the induction of human immunodeficiency virus type 1 replication from primary quiescent CD4 lymphocytes.

1994 • 401 citations

Nef stimulates human immunodeficiency virus type 1 proviral DNA synthesis

1995 • 385 citations

Optimal infectivity in vitro of human immunodeficiency virus type 1 requires an intact nef gene

1994 • 372 citations

The solution structure of HIV-1 Nef reveals an unexpected fold and permits delineation of the binding surface for the SH3 domain of Hck tyrosine protein kinase

1996 • 336 citations

HIV-1 nef leads to inhibition or activation of T cells depending on its intracellular localization

1994 • 305 citations

Downregulation of cell-surface CD4 expression by simian immunodeficiency virus Nef prevents viral super infection.

1993 • 291 citations

Identification of a nef allele that causes lymphocyte activation and acute disease in Macaque monkeys

1995 • 252 citations

Analysis of human immunodeficiency virus type 1 nef gene sequences present in vivo

1993 • 241 citations

Nef protein of human immunodeficiency virus type 1: evidence against its role as a transcriptional inhibitor.

1989 • 227 citations

Requirement of human immunodeficiency virus type 1 nef for in vivo replication and pathogenicity

1994 • 195 citations

Dissociation of the CD4 downregulation and viral infectivity enhancement functions of human immunodeficiency virus type 1 Nef

1995 • 191 citations

CD4 down-regulation by nef alleles isolated from human immunodeficiency virus type 1-infected individuals.

1993 • 186 citations

Human immunodeficiency virus type 1 Nef-induced down-modulation of CD4 is due to rapid internalization and degradation of surface CD4

1994 • 179 citations

Natural HIV-1 NEF accelerates virus replication in primary human lymphocytes

1992 • 158 citations

A Conserved Domain and Membrane Targeting of Nef from HIV and SIV Are Required for Association with a Cellular Serine Kinase Activity

1995 • 122 citations

Nef from primary isolates of human immunodeficiency virus type 1 suppresses surface CD4 expression in human and mouse T cells

1993 • 118 citations

HIV-1 Nef Association with Cellular Serine Kinase Correlates with Enhanced Virion Infectivity and Efficient Proviral DNA Synthesis

1996 • 94 citations

The Role of Nef in the Replication Cycle of the Human and Simian Immunodeficiency Viruses

1994 • 88 citations

Mutational Analysis of HIV-1 Nef: Identification of Two Mutants That Are Temperature-Sensitive for CD4 Downregulation

1996 • 76 citations

Human immunodeficiency virus type 1 Nef protein down-regulates transcription factors NF-kappa B and AP-1 in human T cells in vitro after T-cell receptor stimulation

1994 • 76 citations

Both Virus and Host Components Are Important for the Manifestation of a Nef- Phenotype in HIV-1 and HIV-2

1995 • 55 citations

Mutational analysis of the human immunodeficiency virus type 1 Eli Nef function.

1992 • 49 citations

Serine Phosphorylation-Independent Downregulation of Cell-Surface CD4 by nef

1991 • 38 citations

Sequence requirements for ligand binding and cell surface expression of the Tac antigen, a human interleukin-2 receptor.

1988 • 21 citations