Nitrogen-15 NMR relaxation studies of the FK506 binding protein: Backbone dynamics of the uncomplexed receptor

[6] Use of T7 RNA polymerase to direct expression of cloned genes

1990 • 6,681 citations

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1986 • 6,284 citations

Modified Spin-Echo Method for Measuring Nuclear Relaxation Times

1958 • 5,632 citations

Calcineurin is a common target of cyclophilin-cyclosporin A and FKBP-FK506 complexes

1991 • 4,043 citations

Model-free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules. 1. Theory and range of validity

1982 • 3,662 citations

Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of 1H-1H spin-spin coupling constants in proteins

1983 • 3,150 citations

Model-free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules

1982 • 2,524 citations

Model-free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules. 2. Analysis of experimental results

1982 • 1,800 citations

Backbone dynamics of proteins as studied by nitrogen-15 inverse detected heteronuclear NMR spectroscopy: application to staphylococcal nuclease

1989 • 1,740 citations

Chemistry and Biology of the Immunophilins and Their Immunosuppressive Ligands

1991 • 1,479 citations

Cyclophilin and peptidyl-prolyl cis-trans isomerase are probably identical proteins

1989 • 1,414 citations

Atomic Structures of the Human Immunophilin FKBP-12 Complexes with FK506 and Rapamycin

1993 • 1,202 citations

Clean TOCSY for proton spin system identification in macromolecules

1988 • 1,128 citations

Nuclear association of a T-cell transcription factor blocked by FK-506 and cyclosporin A

1991 • 1,054 citations

Deviations from the simple two-parameter model-free approach to the interpretation of nitrogen-15 nuclear magnetic relaxation of proteins

1990 • 1,044 citations

Structure-Based Strategies for Drug Design and Discovery

1992 • 972 citations

Backbone dynamics of calmodulin studied by nitrogen-15 relaxation using inverse detected two-dimensional NMR spectroscopy: the central helix is flexible

1992 • 880 citations

Cyclosporin A, FK-506, and Rapamycin: Pharmacologic Probes of Lymphocyte Signal Transduction

1992 • 675 citations

Atomic Structure of FKBP-FK506, an Immunophilin-Immunosuppressant Complex

1991 • 641 citations

Intramolecular motions of a zinc finger DNA-binding domain from Xfin characterized by proton-detected natural abundance carbon-13 heteronuclear NMR spectroscopy

1991 • 566 citations

Inhibition of T cell signaling by immunophilin-ligand complexes correlates with loss of calcineurin phosphatase activity

1992 • 561 citations

Comparison of different modes of two-dimensional reverse-correlation NMR for the study of proteins

1990 • 510 citations

Analysis of the backbone dynamics of interleukin-1.beta. using two-dimensional inverse detected heteronuclear nitrogen-15-proton NMR spectroscopy

1990 • 432 citations

Mapping of spectral density functions using heteronuclear NMR relaxation measurements

1992 • 386 citations

Analysis of networks of coupled spins by multiple quantum N.M.R.

1983 • 381 citations

Probing Immunosuppressant Action with a Nonnatural Immunophilin Ligand

1990 • 353 citations

Mapping of the spectral densities of nitrogen-hydrogen bond motions in Eglin c using heteronuclear relaxation experiments

1992 • 313 citations

Solution Structure of FKBP, a Rotamase Enzyme and Receptor for FK506 and Rapamycin

1991 • 302 citations

Solvent suppression using a spin lock in 2D and 3D NMR spectroscopy with H2O solutions

1989 • 290 citations

Structure of human cyclophilin and its binding site for cyclosporin A determined by X-ray crystallography and NMR spectroscopy

1991 • 270 citations

NMR structure of cyclosporin A bound to cyclophilin in aqueous solution

1991 • 268 citations

Studies of chemical exchange by nuclear magnetic relaxation in the rotating frame

1970 • 260 citations

Comparison of techniques for 1H-detected heteronuclear 1H15N Spectroscopy

1990 • 251 citations

Solution structure of the cyclosporin A/cyclophilin complex by NMR

1993 • 241 citations

Broadband spin decoupling in isotropic-liquids

1987 • 234 citations

Design of enzyme inhibitors using iterative protein crystallographic analysis

1991 • 228 citations

Determination of 15N chemical shift tensor via 15N-2H dipolar coupling in Boc-glycylglycyl[15N glycine]benzyl ester

1988 • 228 citations

X-ray structure of a decameric cyclophilin-cyclosporin crystal complex

1993 • 217 citations

Thienothiopyran-2-sulfonamides: novel topically active carbonic anhydrase inhibitors for the treatment of glaucoma

1989 • 197 citations

NMR studies of [U-13C] cyclosporin A bound to cyclophilin: bound conformation and portions of cyclosporin involved in binding

1991 • 194 citations

Backbone dynamics of the Bacillus subtilis glucose permease IIA domain determined from nitrogen-15 NMR relaxation measurements

1992 • 193 citations

Crystal structure of recombinant human T-cell cyclophilin A at 2.5 A resolution.

1991 • 169 citations

Backbone dynamics of calcium-loaded calbindin D9k studied by two-dimensional proton-detected nitrogen-15 NMR spectroscopy

1992 • 166 citations

Fast internal main-chain dynamics of human ubiquitin

1992 • 157 citations

Natural Products as Probes of Cellular Function: Studies of Immunophilins

1992 • 147 citations

Solution structure of the major binding protein for the immunosuppressant FK506

1991 • 146 citations

The X‐ray structure of a tetrapeptide bound to the active site of human cyclophilin A

1992 • 129 citations

Comparison of backbone and tryptophan side-chain dynamics of reduced and oxidized Escherichia coli thioredoxin using nitrogen-15 NMR relaxation measurements

1993 • 119 citations

Loop mobility in a four-helix-bundle protein: nitrogen-15 NMR relaxation measurements on human interleukin-4

1992 • 107 citations

Cyclosporin A—cyclophilin complex formation A model based on X‐ray and NMR data

1992 • 101 citations

Analysis of 1H NMR spectra of proteins using multiple-quantum coherence

1986 • 77 citations

Three-dimensional structure of the FK506 binding protein/ascomycin complex in solution by heteronuclear three- and four-dimensional NMR

1993 • 76 citations

Analysis of the backbone dynamics of the ribonuclease H domain of the human immunodeficiency virus reverse transcriptase using nitrogen-15 relaxation measurements

1992 • 68 citations

Protein secondary structure determination by NMR Application with recombinant human cyclophilin

1991 • 66 citations

Nuclear magnetic resonance studies of the internal dynamics in apo, (Cd2+)1 and (Ca2+)2 Calbindin D9k

1992 • 65 citations

Use of structural information in drug design

1992 • 59 citations

Conformation of two non‐immunosuppressive FK506 analogs when bound to FKBP by isotope‐filtered NMR

1992 • 56 citations

Uniform carbon-13 isotope labeling of proteins with sodium acetate for NMR studies: application to human carbonic anhydrase II

1991 • 54 citations

A model of the cyclophilin/cyclosporin A (CSA) complex from NMR and X-ray data suggests that CSA binds as a transition-state analog

1992 • 52 citations

Proton and nitrogen sequential assignments and secondary structure determination of the human FK506 and rapamycin binding protein

1991 • 50 citations

Backbone dynamics of the glucocorticoid receptor DNA-binding domain

1992 • 48 citations

Solution structure of cyclosporin A and a nonimmunosuppressive analog bound to fully deuterated cyclophilin

1992 • 47 citations

Characterization of the backbone dynamics of an anti‐digoxin antibody V_L domain by inverse detected ¹H–¹⁵N NMR: Comparisons with X‐ray data for the Fab

1993 • 45 citations

Solution structure of FK506 bound to FKBP‐12

1992 • 38 citations

NMR studies of an FK-506 analog, [U-carbon-13]ascomycin, bound to FK-506-binding protein

1992 • 27 citations