Chemical modification of Escherichia coli RNA polymerase by diethyl pyrocarbonate: evidence of histidine requirement for enzyme activity and intrinsic zinc binding

Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.

1985 • 2,450 citations

Refinement of the Coomassie blue method of protein quantitation

1978 • 1,573 citations

Procedure for the rapid, large-scale purification of Escherichia coli DNA-dependent RNA polymerase involving polymin P precipitation and DNA-cellulose chromatography

1975 • 1,157 citations

[37] Reaction of protein sulfhydryl groups with Ellman's reagent

1972 • 892 citations

[41] Modification of histidyl residues in proteins by diethylpyrocarbonate

1977 • 813 citations

Ethoxyformylation of proteins. Reaction of ethoxyformic anhydride with .alpha.-chymotrypsin, pepsin, and pancreatic ribonuclease at pH 4

1970 • 388 citations

Xenopus transcription factor A requires zinc for binding to the 5 S RNA gene.

1983 • 356 citations

Purification and properties of the .sigma. subunit of Escherichia coli DNA-dependent RNA polymerase

1979 • 319 citations

The Presence and Possible Role of Zinc in RNA Polymerase Obtained from Escherichia coli

1971 • 197 citations

Ionic properties of an essential histidine residue in pig heart lactate dehydrogenase

1973 • 176 citations

The Role of Deoxyribonucleic Acid in Ribonucleic Acid Synthesis

1969 • 110 citations

Role of the intrinsic metal in RNA polymerase from Escherichia coli. In vivo substitution of tightly bound zinc with cobalt

1977 • 61 citations

Subunit location of the intrinsic divalent metal ions in RNA polymerase from Escherichia coli

1977 • 57 citations

Rose bengal. Inhibitor of ribonucleic acid chain elongation

1973 • 55 citations

The structure and mechanism of action of bacterial DNA-dependent RNA polymerase

1981 • 48 citations

Correlations between biological activity and the number of functional groups chemically modified

1979 • 47 citations

[42] Purification of the RNA polymerase sigma factor

1971 • 41 citations

Nuclear magnetic resonance studies on the role of intrinsic metals in Escherichia coli RNA polymerase. Effect of DNA template on the nucleotide-enzyme interaction.

1984 • 38 citations

Subunit localizations of zinc(II) in DNA-dependent RNA polymerase from Escherichia coli B

1979 • 37 citations

Selective substitution in vitro of an intrinsic zinc of Escherichia coli RNA polymerase with various divalent metals

1982 • 34 citations

Electron microscope studies of muscle structure

1948 • 33 citations

Direct coordination of nucleotide with the intrinsic metal in Escherichia coli RNA polymerase. A nuclear magnetic resonance study with cobalt-substituted enzyme

1982 • 32 citations

Nuclear magnetic resonance and chemical modification studies of the role of the metal in the yeast aldolase

1981 • 25 citations

The reaction of diethyl pyrocarbonate with pyruvate kinase

1974 • 24 citations

DNA-activated ATPase activity associated with Xenopus transcription factor A.

1986 • 20 citations

Intrinsic zinc ion is essential for proper conformation of active Escherichia coli RNA polymerase

1984 • 19 citations

Mechanistic aspects of promoter binding and chain initiation by RNA polymerase

1982 • 18 citations

Preparation and characterization of various Escherichia coli RNA polymerases containing one or two intrinsic metal ions

1985 • 16 citations