Conformational changes of the leukocyte NADPH oxidase subunit p47phox during activation studied through its intrinsic fluorescence

Principles of Fluorescence Spectroscopy

1983 • 5,123 citations

Solute perturbation of protein fluorescence. Quenching of the tryptophyl fluorescence of model compounds and of lysozyme by iodide ion

1971 • 1,730 citations

Exposure of tryptophanyl residues in proteins. Quantitative determination by fluorescence quenching studies

1976 • 1,020 citations

Regulation of Phagocyte Oxygen Radical Production by the GTP-Binding Protein Rac 2

1991 • 669 citations

Two cytosolic components of the human neutrophil respiratory burst oxidase translocate to the plasma membrane during cell activation.

1990 • 435 citations

Neutrophil nicotinamide adenine dinucleotide phosphate oxidase assembly. Translocation of p47-phox and p67-phox requires interaction between p47-phox and cytochrome b558.

1991 • 379 citations

The phosphorylation of the respiratory burst oxidase component p47phox during neutrophil activation. Phosphorylation of sites recognized by protein kinase C and by proline-directed kinases.

1994 • 342 citations

Unsaturated fatty acids stimulate NADPH-dependent superoxide production by cell-free system derived from macrophages

1984 • 335 citations

Translocation of Rac correlates with NADPH oxidase activation. Evidence for equimolar translocation of oxidase components.

1993 • 316 citations

Role of Src homology 3 domains in assembly and activation of the phagocyte NADPH oxidase.

1994 • 286 citations

NADPH oxidase of human neutrophils. Subcellular localization and characterization of an arachidonate-activatable superoxide-generating system.

1987 • 266 citations

Reconstitution of neutrophil NADPH oxidase activity in the cell-free system by four components: p67-phox, p47-phox, p21rac1, and cytochrome b-245.

1992 • 265 citations

Phosphorylation of neutrophil 47-kDa cytosolic oxidase factor. Translocation to membrane is associated with distinct phosphorylation events.

1990 • 248 citations

Activation of p38 in Stimulated Human Neutrophils: Phosphorylation of the Oxidase Component p47 by p38 and ERK but Not by JNK

1996 • 248 citations

Amyloid β-Protein Inhibits Ubiquitin-dependent Protein Degradation in Vitro

1995 • 223 citations

The phosphorylation targets of p47phox, a subunit of the respiratory burst oxidase. Functions of the individual target serines as evaluated by site-directed mutagenesis.

1995 • 184 citations

Purification and characterization of Rac 2. A cytosolic GTP-binding protein that regulates human neutrophil NADPH oxidase.

1992 • 176 citations

A possible role for protein phosphorylation in the activation of the respiratory burst in human neutrophils. Evidence from studies with cells from patients with chronic granulomatous disease.

1986 • 168 citations

The cytosolic components of the respiratory burst oxidase exist as a M(r) approximately 240,000 complex that acquires a membrane-binding site during activation of the oxidase in a cell-free system.

1992 • 150 citations

Multiple SH3 domain interactions regulate NADPH oxidase assembly in whole cells.

1996 • 116 citations

Activation of the Superoxide Forming NADPH Oxidase in a Cell-free System by Sodium Dodecyl Sulfate

1989 • 111 citations

Mapping the domains of interaction of p40phox with both p47phox and p67phox of the neutrophil oxidase complex using the two-hybrid system.

1995 • 106 citations

Isolation of a Complex of Respiratory Burst Oxidase Components from Resting Neutrophil Cytosol

1994 • 96 citations

Kinase-dependent Activation of the Leukocyte NADPH Oxidase in a Cell-free System

1997 • 81 citations

Mapping the Domains of Interaction of p40 with Both p47 and p67 of the Neutrophil Oxidase Complex Using the Two-hybrid System

1995 • 81 citations

Direct evidence for interaction between COOH-terminal regions of cytochrome b558 subunits and cytosolic 47-kDa protein during activation of an O(2-)-generating system in neutrophils.

1992 • 78 citations

Activation of the Leukocyte NADPH Oxidase Subunit p47^phox by Protein Kinase C. A Phosphorylation-Dependent Change in the Conformation of the C-Terminal End of p47^phox

1997 • 71 citations

Cell-free translocation of recombinant p47-phox, a component of the neutrophil NADPH oxidase: effects of guanosine 5'-O-(3-thiotriphosphate), diacylglycerol, and an anionic amphiphile

1992 • 43 citations

Cell-Free Activation of the Respiratory Burst Oxidase by Protein Kinase C

1995 • 36 citations

Cytosolic factors in bovine neutrophil oxidase activation. Partial purification and demonstration of translocation to a membrane fraction

1990 • 31 citations

Translocation of Recombinant p47phox Cytosolic Component of the Phagocyte Oxidase by in Vitro Phosphorylation

1995 • 21 citations