<scp>STED</scp> microscopy of living cells – new frontiers in membrane and neurobiology

Two-Photon Laser Scanning Fluorescence Microscopy

1990 • 9,129 citations

Imaging Intracellular Fluorescent Proteins at Nanometer Resolution

2006 • 8,251 citations

Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)

2006 • 7,599 citations

Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy

1994 • 5,569 citations

Lipid Rafts As a Membrane-Organizing Principle

2009 • 4,078 citations

Ultra-High Resolution Imaging by Fluorescence Photoactivation Localization Microscopy

2006 • 3,456 citations

Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy

2000 • 3,436 citations

Far-Field Optical Nanoscopy

2007 • 2,876 citations

Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy

2008 • 2,696 citations

Beiträge zur Theorie des Mikroskops und der mikroskopischen Wahrnehmung

1873 • 2,407 citations

Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution

2005 • 2,115 citations

Subdiffraction‐Resolution Fluorescence Imaging with Conventional Fluorescent Probes

2008 • 1,794 citations

Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission

2000 • 1,736 citations

Thermodynamic Fluctuations in a Reacting System—Measurement by Fluorescence Correlation Spectroscopy

1972 • 1,704 citations

Super-Resolution Fluorescence Microscopy

2009 • 1,563 citations

Direct observation of the nanoscale dynamics of membrane lipids in a living cell

2008 • 1,461 citations

Multicolor Super-Resolution Imaging with Photo-Switchable Fluorescent Probes

2007 • 1,436 citations

Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging

2011 • 1,270 citations

Fluorescent probes for super-resolution imaging in living cells

2008 • 1,249 citations

Morphological Changes in Dendritic Spines Associated with Long-Term Synaptic Plasticity

2001 • 1,236 citations

High-density mapping of single-molecule trajectories with photoactivated localization microscopy

2008 • 1,167 citations

STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis

2006 • 1,148 citations

Breaking the Diffraction Barrier: Super-Resolution Imaging of Cells

2010 • 1,110 citations

Subdiffraction Multicolor Imaging of the Nuclear Periphery with 3D Structured Illumination Microscopy

2008 • 1,099 citations

Paradigm Shift of the Plasma Membrane Concept from the Two-Dimensional Continuum Fluid to the Partitioned Fluid: High-Speed Single-Molecule Tracking of Membrane Molecules

2005 • 1,086 citations

Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI)

2009 • 1,047 citations

Microscopy and its focal switch

2008 • 997 citations

Toward fluorescence nanoscopy

2003 • 979 citations

Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function

2009 • 955 citations

Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure

2009 • 863 citations

A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins

2013 • 844 citations

Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics

2008 • 840 citations

Optimized localization analysis for single-molecule tracking and super-resolution microscopy

2010 • 837 citations

Three-dimensional sub–100 nm resolution fluorescence microscopy of thick samples

2008 • 799 citations

Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins

2005 • 797 citations

Video-Rate Far-Field Optical Nanoscopy Dissects Synaptic Vesicle Movement

2008 • 777 citations

Fluorescence nanoscopy by ground-state depletion and single-molecule return

2008 • 747 citations

Fast, three-dimensional super-resolution imaging of live cells

2011 • 732 citations

Photobleaching of Fluorescent Dyes under Conditions Used for Single-Molecule Detection: Evidence of Two-Step Photolysis

1998 • 695 citations

Superresolution Imaging of Chemical Synapses in the Brain

2010 • 691 citations

Saturated patterned excitation microscopy—a concept for optical resolution improvement

2002 • 554 citations

Ground-state-depletion fluorscence microscopy: A concept for breaking the diffraction resolution limit

1995 • 518 citations

Fast, single-molecule localization that achieves theoretically minimum uncertainty

2010 • 516 citations

Superresolution Imaging using Single-Molecule Localization

2010 • 513 citations

STED microscopy with continuous wave beams

2007 • 506 citations

Diffraction-unlimited all-optical imaging and writing with a photochromic GFP

2011 • 468 citations

Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell

2008 • 458 citations

Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton

2012 • 455 citations

Anatomy and Dynamics of a Supramolecular Membrane Protein Cluster

2007 • 449 citations

Fluorescence Correlation Spectroscopy Diffusion Laws to Probe the Submicron Cell Membrane Organization

2005 • 430 citations

Spherical nanosized focal spot unravels the interior of cells

2008 • 421 citations

Bayesian localization microscopy reveals nanoscale podosome dynamics

2011 • 419 citations

Sharper low-power STED nanoscopy by time gating

2011 • 412 citations

Live-cell imaging of dendritic spines by STED microscopy

2008 • 393 citations

Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution

2011 • 380 citations

Single-molecule high-resolution imaging with photobleaching

2004 • 366 citations

Nanoscopy in a Living Mouse Brain

2012 • 366 citations

Nanoscale resolution in GFP-based microscopy

2006 • 363 citations

Rotational brownian motion and fluorescence intensify fluctuations

1974 • 363 citations

Focal Spots of Size<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mi>λ</mml:mi><mml:mi>/</mml:mi><mml:mn>23</mml:mn></mml:math>Open Up Far-Field Florescence Microscopy at 33 nm Axial Resolution

2002 • 357 citations

Superresolution by localization of quantum dots using blinking statistics

2005 • 356 citations

Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP

2008 • 341 citations

Breaking the Diffraction Barrier in Fluorescence Microscopy by Optical Shelving

2007 • 335 citations

Subnanometre single-molecule localization, registration and distance measurements

2010 • 324 citations

Partitioning, diffusion, and ligand binding of raft lipid analogs in model and cellular plasma membranes

2012 • 317 citations

Simultaneous multiple-emitter fitting for single molecule super-resolution imaging

2011 • 308 citations

Adaptive optics enables 3D STED microscopy in aberrating specimens

2012 • 306 citations

Nanometer-localized multiple single-molecule fluorescence microscopy

2004 • 299 citations

STED Nanoscopy of Actin Dynamics in Synapses Deep Inside Living Brain Slices

2011 • 290 citations

A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching

2011 • 282 citations

STED microscopy with a supercontinuum laser source

2008 • 273 citations

Concepts for nanoscale resolution in fluorescence microscopy

2004 • 269 citations

Molecular Photobleaching Kinetics of Rhodamine 6G by One‐ and Two‐Photon Induced Confocal Fluorescence Microscopy

2005 • 253 citations

STED Nanoscopy Reveals Molecular Details of Cholesterol- and Cytoskeleton-Modulated Lipid Interactions in Living Cells

2011 • 250 citations

Photoswitches: Key molecules for subdiffraction‐resolution fluorescence imaging and molecular quantification

2009 • 247 citations

Ultrasensitive investigations of biological systems by fluorescence correlation spectroscopy

2003 • 245 citations

Two-photon excitation STED microscopy

2009 • 226 citations

Real‐time computation of subdiffraction‐resolution fluorescence images

2009 • 219 citations

rsEGFP2 enables fast RESOLFT nanoscopy of living cells

2012 • 217 citations

Super-resolution microscopy by nanoscale localization of photo-switchable fluorescent probes

2008 • 204 citations

Three-Dimensional Nanoscopy of Colloidal Crystals

2008 • 192 citations

Imaging and writing at the nanoscale with focused visible light through saturable optical transitions

2003 • 191 citations

Polarization effect on position accuracy of fluorophore localization

2006 • 190 citations

Subdiffraction resolution in continuous samples

2009 • 184 citations

Fast molecular tracking maps nanoscale dynamics of plasma membrane lipids

2010 • 183 citations

Putting super-resolution fluorescence microscopy to work

2008 • 182 citations

Supraresolution Imaging in Brain Slices using Stimulated-Emission Depletion Two-Photon Laser Scanning Microscopy

2009 • 171 citations

Strategy for far-field optical imaging and writing without diffraction limit

2004 • 169 citations

Hierarchical organization of the plasma membrane: Investigations by single‐molecule tracking vs. fluorescence correlation spectroscopy

2010 • 168 citations

Endosomal sorting of readily releasable synaptic vesicles

2010 • 164 citations

Spatial distribution of Na+-K+-ATPase in dendritic spines dissected by nanoscale superresolution STED microscopy

2011 • 160 citations

Super-resolution optical microscopy: multiple choices

2009 • 158 citations

Molecular Orientation Affects Localization Accuracy in Superresolution Far-Field Fluorescence Microscopy

2010 • 157 citations

Two-color far-field fluorescence nanoscopy based on photoswitchable emitters

2007 • 155 citations

STED Microscopy and its Applications: New Insights into Cellular Processes on the Nanoscale

2012 • 151 citations

&lt;title&gt;Sevenfold improvement of axial resolution in 3D wide-field microscopy using two objective lenses&lt;/title&gt;

1995 • 147 citations

Metastable Dark States Enable Ground State Depletion Microscopy of Nitrogen Vacancy Centers in Diamond with Diffraction-Unlimited Resolution

2010 • 138 citations

STED microscopy detects and quantifies liquid phase separation in lipid membranes using a new far-red emitting fluorescent phosphoglycerolipid analogue

2012 • 138 citations

Super-Resolution Dynamic Imaging of Dendritic Spines Using a Low-Affinity Photoconvertible Actin Probe

2011 • 137 citations

Stimulated Emission Depletion Nanoscopy of Living Cells Using SNAP-Tag Fusion Proteins

2010 • 128 citations

Two-Color STED Microscopy of Living Synapses Using A Single Laser-Beam Pair

2011 • 128 citations

Two-color STED microscopy in living cells

2011 • 127 citations

(Un)Confined Diffusion of CD59 in the Plasma Membrane Determined by High-Resolution Single Molecule Microscopy

2007 • 127 citations

Nanoscopy of Living Brain Slices with Low Light Levels

2012 • 127 citations

Wide‐field subdiffraction RESOLFT microscopy using fluorescent protein photoswitching

2007 • 120 citations

Fast STED microscopy with continuous wave fiber lasers

2010 • 112 citations

Spectroscopic Rationale for Efficient Stimulated-Emission Depletion Microscopy Fluorophores

2010 • 104 citations

Molecular layers underlying cytoskeletal remodelling during cortical development

2009 • 100 citations

Nanoscopy in a Living Multicellular Organism Expressing GFP

2011 • 95 citations

A STED microscope aligned by design

2009 • 87 citations

Investigating Sub-Spine Actin Dynamics in Rat Hippocampal Neurons with Super-Resolution Optical Imaging

2009 • 87 citations

Two-photon excitation in functional biological imaging

1996 • 84 citations

Exploring single-molecule dynamics with fluorescence nanoscopy

2009 • 84 citations

STED Nanoscopy in Living Cells Using Fluorogen Activating Proteins

2009 • 78 citations

Super-resolution microscopy: breaking the limits

2008 • 68 citations

Characterization via Two-Color STED Microscopy of Nanostructured Materials Synthesized by Colloid Electrospinning

2011 • 60 citations

Triplet-relaxation microscopy with bunched pulsed excitation

2009 • 58 citations

Subdiffraction-Limit Two-Photon Fluorescence Microscopy for GFP-Tagged Cell Imaging

2009 • 57 citations

Breaking the Resolution Limit in Light Microscopy

2007 • 52 citations

In Vivo Labeling Method Using a Genetic Construct for Nanoscale Resolution Microscopy

2009 • 49 citations

Subdiffraction‐Resolution Fluorescence Microscopy of Myosin–Actin Motility

2010 • 41 citations

Diffraction-unlimited optical microscopy

2008 • 38 citations

Primer: fluorescence imaging under the diffraction limit

2008 • 36 citations

STED nanoscopy with mass-produced laser diodes

2011 • 35 citations

Beyond the diffraction limit: far-field fluorescence imaging with ultrahigh resolution

2007 • 33 citations

Fast scanning STED and two‐photon fluorescence excitation microscopy with continuous wave beam

2011 • 21 citations

Lucky Imaging: Improved Localization Accuracy for Single Molecule Imaging

2009 • 14 citations

STED-FCS Nanoscopy of Membrane Dynamics

2012 • 10 citations

Super-resolution STED microscopy advances with yellow CW OPSL

2012 • 7 citations

Ground State Depletion Fluorescence Microscopy

2008 • 2 citations