The quantification of gene expression in an animal model of brain ischaemia using TaqMan™ real-time RT-PCR

Real time quantitative PCR.

1996 • 6,155 citations

Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.

1991 • 2,688 citations

Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions

1993 • 2,177 citations

A novel method for real time quantitative RT-PCR.

1996 • 2,093 citations

Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.

1995 • 1,619 citations

Soft modelling: The Basic Design and Some Extensions

1982 • 1,295 citations

Simultaneous Amplification and Detection of Specific DNA Sequences

1992 • 1,108 citations

Experimental studies of ischemic brain edema

1986 • 932 citations

Cytokines and the nervous system II: actions and mechanisms of action

1995 • 928 citations

Experimental studies of ischemic brain edema : a new experimental study of ischemic brain edema : 1. A new experimental model of cerebral embolism in rats in which recirculation can be introduced in the ischemic area

1986 • 770 citations

Allelic discrimination by nick-translation PCR with fluorgenic probes

1993 • 756 citations

Cytokines and the nervous system I: expression and recognition

1995 • 745 citations

Lectures on Biostatistics.

1972 • 551 citations

Interleukin-1 receptor antagonist inhibits ischaemic and excitotoxic neuronal damage in the rat

1992 • 547 citations

Direct Comparison of GAPDH, β-Actin, Cyclophilin, and 28S rRNA as Internal Standards for Quantifying RNA Levels under Hypoxia

1999 • 466 citations

Neuropeptide expression in rat dorsal root ganglion cells and spinal cord after peripheral nerve injury with special reference to galanin

1989 • 460 citations

Lectures on biostatistics

1971 • 419 citations

Interleukin-1 beta mRNA expression in ischemic rat cortex.

1993 • 335 citations

Inducible Nitric Oxide Synthase Gene Expression in Vascular Cells After Transient Focal Cerebral Ischemia

1996 • 331 citations

Cytokines and the nervous system I: expression and recognition

1995 • 307 citations

The use of TaqMan RT-PCR assays for semiquantitative analysis of gene expression in CNS tissues and disease models

2000 • 265 citations

Advances in quantitative PCR technology: 5′ nuclease assays

1998 • 256 citations

Expression of tumor necrosis factor alpha after focal cerebral ischaemia in the rat

1996 • 247 citations

Nestin expression in reactive astrocytes following focal cerebral ischemia in rats

1997 • 208 citations

Upregulation of neuronal nitric oxide synthase and mRNA, and selective sparing of nitric oxide synthase-containing neurons after local cerebral ischemia in rat

1994 • 200 citations

Astrocytic demise precedes delayed neuronal death in focal ischemic rat brain

1999 • 179 citations

Expression of Interleukin-6, c-Fos, and zif268 mRNAs in Rat Ischemic Cortex

1995 • 179 citations

Problems related to the interpretation of autoradiographic data on gene expression using common constitutive transcripts as controls

1993 • 178 citations

Acute Focal Ischemia-Induced Alterations in MAP2 Immunostaining: Description of Temporal Changes and Utilization as a Marker for Volumetric Assessment of Acute Brain Injury

1996 • 177 citations

Temporal profile of nestin expression after focal cerebral ischemia in adult rat

1999 • 141 citations

Leukemia Inhibitory Factor Is Expressed in Astrocytes Following Cortical Brain Injury

1997 • 130 citations

Cranial motor neurons contain either galanin‐ or calcitonin gene‐related peptidelike immunoreactivity

1989 • 128 citations

Gene expression of IL-10 in relationship to TNF-α, IL-1β and IL-2 in the rat brain following middle cerebral artery occlusion

1997 • 126 citations

Adjusting for baseline: Change or percentage change?

1989 • 120 citations

The use of quantitative RT-PCR to measure mRNA expression in a rat model of focal ischemia — caspase-3 as a case study

2000 • 114 citations

Pcr-Based Diagnostics in Infectious Disease

1994 • 87 citations

High-resolution Quantification of Specific mRNA Levels in Human Brain Autopsies and Biopsies

2000 • 80 citations

Galanin expression increases in adult rat sympathetic neurons after axotomy

1994 • 74 citations

Immunohistochemical Detection of Leukemia Inhibitory Factor After Focal Cerebral Ischemia in Rats

2000 • 62 citations

Transcription of actin, cyclophilin and glyceraldehyde phosphate dehydrogenase genes: tissue- and treatment-specificity

1999 • 56 citations

Evaluation of quantitative variation in gene expression

1994 • 39 citations

Pixel-based image analysis of HSP70, GADD45 and MAP2 mRNA expression after focal cerebral ischemia: hemodynamic and histological correlates

1998 • 35 citations

Pattern Recognition Analysis of Endogenous Cell Metabolites for High Throughput Mode of Action Identification: Removing the Postscreening Dilemma Associated with Whole-Organism High Throughput Screening

2000 • 21 citations

Leukemia inhibitory factor regulates galanin/galanin message-associated peptide expression in cultured mouse dorsal root ganglia; with a note on in situ hybridization methodology

1999 • 17 citations