Molecular cloning and sequence determination of rat preproenkephalin cDNA: sensitive probe for studying transcriptional changes in rat tissues.

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

[57] Sequencing end-labeled DNA with base-specific chemical cleavages

1980 • 15,302 citations

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I

1977 • 12,015 citations

Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

1980 • 8,711 citations

Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose

1972 • 6,922 citations

A simple and very efficient method for generating cDNA libraries

1983 • 4,606 citations

Identification of two related pentapeptides from the brain with potent opiate agonist activity

1975 • 3,780 citations

Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples.

1982 • 1,333 citations

Methylmercury as a reversible denaturing agent for agarose gel electrophoresis

1976 • 1,323 citations

Plasmid screening at high colony density

1980 • 1,100 citations

Cloning and sequence analysis of cDNA for bovine adrenal preproenkephalin

1982 • 928 citations

Primary structure of the human Met- and Leu-enkephalin precursor and its mRNA

1982 • 672 citations

Molecular cloning establishes proenkephalin as precursor of enkephalin-containing peptides

1982 • 621 citations

PROCESSING MECHANISMS IN THE BIOSYNTHESIS OF PROTEINS*

1980 • 461 citations

Effect of Growth Conditions on the Formation of the Relaxation Complex of Supercoiled ColE1 Deoxyribonucleic Acid and Protein in Escherichia coli

1972 • 351 citations

Regional distribution of leu and met enkephalin in rat brain

1977 • 307 citations

Biochemistry of the enkephalins and enkephalin-containing peptides

1983 • 270 citations

A highly potent 3200-dalton adrenal opioid peptide that contains both a [Met]- and [Leu]enkephalin sequence.

1981 • 133 citations

Transformation of E. coli using homopolymer-linked plasmid chimeras

1981 • 99 citations

Characterization of the homopolymer tailing reaction catalyzed by terminal deoxynucleotidyl transferase. Implications for the cloning of cDNA.

1982 • 98 citations

Marked increases in large enkephalin-containing polypeptides in the rat adrenal gland following denervation

1981 • 82 citations

Solid-phase synthesis of polynucleotides. IV. Usage of polystyrene resins for the synthesis of polydeoxyribonucleotides by the phosphotriester method

1980 • 77 citations

Brain enkephalin distribution is unaltered by hypophysectomy

1978 • 76 citations

Denervation of rat adrenal glands markedly increases preproenkephalin mRNA.

1984 • 67 citations

Changes in rat adrenal catecholamines and proenkephalin metabolism after denervation.

1984 • 52 citations

mRNA species directing synthesis of milk proteins in normal and tumour tissue from human mammary gland

1979 • 37 citations