Introns increase gene expression in cultured maize cells.

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1983 • 26,063 citations

Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.

1982 • 8,069 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

Firefly luciferase gene: structure and expression in mammalian cells.

1987 • 3,088 citations

Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.

1982 • 2,753 citations

Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.

1983 • 2,456 citations

Why genes in pieces?

1978 • 2,256 citations

SPLICING OF MESSENGER RNA PRECURSORS

1986 • 1,233 citations

Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5

1982 • 936 citations

Splicing of Messenger RNA Precursors

1987 • 919 citations

Gene regulation by proteins acting nearby and at a distance

1986 • 886 citations

Splicing of Messenger RNA Precursors

1984 • 874 citations

Expression of bacterial genes in plant cells.

1983 • 836 citations

Expression of genes transferred into monocot and dicot plant cells by electroporation.

1985 • 749 citations

Agrobacterium rhizogenes inserts T-DNA into the genomes of the host plant root cells

1982 • 707 citations

Stable transformation of maize after gene transfer by electroporation

1986 • 638 citations

A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation

1983 • 594 citations

The role of small nuclear ribonucleoprotein particles in pre-mRNA splicing

1987 • 594 citations

Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector

1983 • 581 citations

The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene

1980 • 556 citations

COMPLEX TRANSCRIPTIONAL UNITS: DIVERSITY IN GENE EXPRESSION BY ALTERNATIVE RNA PROCESSING

1986 • 486 citations

Genes in pieces: were they ever together?

1978 • 440 citations

Transformation of rat cells by an altered polyoma virus genome expressing only the middle-T protein

1981 • 435 citations

A new sensitive method for qualitative and quantitative assay of neomycin phosphotransferase in crude cell extracts

1984 • 398 citations

Histone Genes and Histone Messengers

1979 • 373 citations

Cloning of the bronze locus in maize by a simple and generalizable procedure using the transposable controlling element Activator ( Ac )

1984 • 365 citations

Molecular analysis of the alcohol dehydrogenase 2 (Adh2) gene of maize

1985 • 338 citations

Strcuture and transcription of the nopaline synthase gene region of T-DNA

1983 • 334 citations

Implications of RNA⋅RNA Splicing in Evolution of Eukaryotic Cells

1978 • 333 citations

DNA modification of a maize transposable element correlates with loss of activity.

1986 • 331 citations

Nopaline synthase: transcript mapping and DNA sequence.

1982 • 322 citations

Size heterogeneity in the 3′ end of dihydrofolate reductase messenger RNAs in mouse cells

1980 • 288 citations

Splicing and the formation of stable RNA

1979 • 274 citations

Ca lectin gene insertion has the structural features of a transposable element

1983 • 259 citations

Comparison of Total Sequence of a Cloned Rabbit β-Globin Gene and Its Flanking Regions with a Homologous Mouse Sequence

1979 • 256 citations

Cloning and sequence analysis reveal structural variation among related zein genes in maize

1982 • 235 citations

[3] An integrated and simplified approach to cloning into plasmids and single-stranded phages

1983 • 210 citations

Molecular analysis of the alcohol dehydrogenase (Adhl) gene of maize

1984 • 207 citations

Monocot and dicot pre-mRNAs are processed with different efficiencies in transgenic tobacco

1986 • 192 citations

Splicing as a requirement for biogenesis of functional 16S mRNA of simian virus 40.

1979 • 185 citations

Maize Adh-1 promoter sequences control anaerobic regulation: addition of upstream promoter elements from constitutive genes is necessary for expression in tobacco

1987 • 168 citations

Simian virus 40 early- and late-region promoter functions are enhanced by the 72-base-pair repeat inserted at distant locations and inverted orientations.

1983 • 149 citations

SV40 recombinants carrying rabbit β-globin gene coding sequences

1979 • 139 citations

ALCOHOL DEHYDROGENASE POLYMORPHISM IN MAIZE—SIMPLE AND COMPOUND LOCI

1966 • 137 citations

DNA insertion in the first intron of maize Adh1 affects message levels: cloning of progenitor and mutant Adh1 alleles.

1984 • 137 citations

[21] Electroporation of DNA and RNA into plant protoplasts

1987 • 129 citations

Expression of abbreviated mouse dihydrofolate reductase genes in cultured hamster cells.

1982 • 121 citations

MAIZE Adhl

1985 • 120 citations

Genetic control of UDPglucose:flavonol 3-O-glucosyltransferase in the endosperm of maize

1977 • 99 citations

TWO ALLELES OF MAIZE ALCOHOL DEHYDROGENASE 1 HAVE 3' STRUCTURAL AND POLY(A) ADDITION POLYMORPHISMS

1986 • 97 citations

Rescue of a splicing defective mutant by insertion of an heterologous intron

1980 • 85 citations

Deletion mutants of simian virus 40 defective in biosynthesis of late viral mRNA.

1979 • 77 citations

Size and position of intervening sequences are critical for the splicing efficiency of pre-mRNA in the yeastSaccharomyces cerevisiae

1985 • 62 citations

Expression of mRNA electroporated into plant and animal cells

1987 • 50 citations

Expression of a bean storage protein ‘phaseolin minigene’ in foreign plant tissues

1986 • 47 citations

Dimerization of multiple maize ADHs studied in vivo and in vitro

1974 • 39 citations

IDENTIFICATION OF A GENETIC ELEMENT THAT CONTROLS THE ORGAN-SPECIFIC EXPRESSION OF Adh1 IN MAIZE

1981 • 35 citations

Synthesis of Predominantly Unspliced Cytoplasmic RNAs by Chimeric Herpes Simplex Virus Type 1 Thymidine Kinase-Human β-Globin Genes

1985 • 31 citations