The pFF plasmids: cassettes utilising CaMV sequences for expression of foreign genes in plants

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

1987 • 9,696 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Assaying chimeric genes in plants: The GUS gene fusion system

1987 • 4,516 citations

[1] Production of single-stranded plasmid DNA

1987 • 2,642 citations

Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter

1985 • 1,387 citations

beta-Glucuronidase from Escherichia coli as a gene-fusion marker.

1986 • 993 citations

Oligonucleotide-Directed Mutagenesis: A Simple Method Using Two Oligonucleotide Primers and a Single-Stranded DNA Template

1984 • 947 citations

Duplication of CaMV 35 S Promoter Sequences Creates a Strong Enhancer for Plant Genes

1987 • 887 citations

Expression of bacterial genes in plant cells.

1983 • 836 citations

Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae

1983 • 810 citations

Expression of genes transferred into monocot and dicot plant cells by electroporation.

1985 • 749 citations

The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation

1984 • 718 citations

Introns increase gene expression in cultured maize cells.

1987 • 703 citations

A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation

1983 • 594 citations

Direct gene transfer to plants

1984 • 586 citations

The CaMV 35S enhancer contains at least two domains which can confer different developmental and tissue-specific expression patterns

1989 • 509 citations

Chimeric genes as dominant selectable markers in plant cells

1983 • 487 citations

A set of plant expression vectors for transcriptional and translational fusions

1987 • 449 citations

The 5'-leader sequence of tobacco mosaic virus RNA enhances the expression of foreign gene transcriptsin vitroandin vivo

1987 • 409 citations

The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by M13mp7 shotgun sequencing

1981 • 402 citations

Regulation of Gene Expression in Higher Plants

1987 • 401 citations

Transcription of cauliflower mosaic virus DNA: detection of promoter sequences, and characterization of transcripts

1982 • 332 citations

Factors Influencing Gene Delivery into Zea Mays Cells by High–Velocity Microprojectiles

1988 • 314 citations

Multiple cis regulatory elements for maximal expression of the cauliflower mosaic virus 35S promoter in transgenic plants.

1989 • 305 citations

Expression in plants of two bacterial antibiotic resistance genes after protoplast transformation with a new plant expression vector

1986 • 299 citations

Enhanced translation of chimaeric messenger RNAs containing a plant viral untranslated leader sequence

1987 • 284 citations

Comparison of cauliflower mosaic virus 35S and nopaline synthase promoters in transgenic plants

1987 • 232 citations

Stable genetic transformation of intact Nicotiana cells by the particle bombardment process

1988 • 226 citations

A chimaeric hygromycin resistance gene as a selectable marker in plant cells

1985 • 209 citations

Resistance to hygromycin B

1985 • 209 citations

Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector

1984 • 199 citations

Functional regions of the cauliflower mosaic virus 35S RNA promoter determined by use of the firefly luciferase gene as a reporter of promoter activity

1987 • 197 citations

High mutation rate of a spleen necrosis virus-based retrovirus vector.

1986 • 157 citations

A short conserved sequence is involved in the light-inducibility of a gene encoding ribulose 1,5-bisphosphate carboxylase small subunit of pea

1985 • 155 citations

Expression of a foreign gene linked to either a plant-virus or a Drosophila promoter, after electroporation of protoplasts of rice, wheat, and sorghum

1986 • 147 citations

Different 3' end regions strongly influence the level of gene expression in plant cells.

1989 • 139 citations

Relative strengths of the 35S califlower mosaic virus, 1′, 2′, and nopaline synthase promoters in transformed tobacco sugarbeet and oilseed rape callus tissue

1988 • 128 citations

Light-Regulated Expression of a Pea Ribulose-1,5-Bisphosphate Carboxylase Small Subunit Gene in Transformed Plant Cells

1984 • 120 citations

Characterisation of cauliflower mosaic virus DNA sequences which encode major polyadenylated transcripts

1981 • 115 citations

Characterisation of the 5′-leader sequence of tobacco mosaic virus RNA as a general enhancer of translation in vitro

1987 • 111 citations

Properties of an isolated transcription stimulating sequence derived from the cauliflower mosaic virus 35S promoter

1988 • 90 citations

Cauliflower mosaic virus 35 S promoter directs S phase specific expression in plant cells

1987 • 54 citations

Different 3' End Regions Strongly Influence the Level of Gene Expression in Plant Cells

1989 • 25 citations

Evidence for ribosome scanning during translation initiation of mRNAs in transformed plant cells

1985 • 20 citations