Substrate Phage: Selection of Protease Substrates by Monovalent Phage Display

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

On the size of the active site in proteases. I. Papain

1967 • 5,150 citations

Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.

1987 • 5,066 citations

High efficiency transformation of E.coli by high voltage electroporation

1988 • 2,789 citations

Serine Proteases: Structure and Mechanism of Catalysis

1977 • 1,238 citations

Structure of Subtilisin BPN′ at 2.5 Å Resolution

1969 • 543 citations

Generation of β-globin by sequence-specific proteolysis of a hybrid protein produced in Escherichia coli

1984 • 374 citations

An Escherichia coli mutation preventing degradation of abnormal periplasmic proteins.

1988 • 374 citations

Hormone phage: An enrichment method for variant proteins with altered binding properties

1990 • 360 citations

The HIV-1 Protease as a Therapeutic Target for AIDS

1992 • 343 citations

Selecting high-affinity binding proteins by monovalent phage display

1991 • 312 citations

[28] Assay of coagulation proteases using peptide chromogenic and fluorogenic substrates

1981 • 310 citations

Correlation between sites of limited proteolysis and segmental mobility in thermolysin

1986 • 297 citations

Highly sensitive peptide‐4‐methylcoumaryl‐7‐amide substrates for blood‐clotting proteases and trypsin

1988 • 245 citations

Probing Steric and Hydrophobic Effects on Enzyme-Substrate Interactions by Protein Engineering

1986 • 240 citations

Engineering Enzyme Specificity by "Substrate-Assisted Catalysis"

1987 • 219 citations

Proteolytic processing and physiological regulation

1989 • 195 citations

The human growth hormone receptor. Secretion from Escherichia coli and disulfide bonding pattern of the extracellular binding domain.

1990 • 164 citations

High-level secretion of human growth hormone by Escherichia coli

1987 • 150 citations

[12] Gene fusions for purpose of expression: An introduction

1990 • 145 citations

Importance of hydrogen-bond formation in stabilizing the transition state of subtilisin

1986 • 136 citations

Engineering subtilisin BPN′ for site‐specific proteolysis

1989 • 110 citations

Post-translational modifications of proteins: some problems left to solve

1989 • 86 citations

Crystal structure of a bacterial protein proteinase inhibitor (Streptomyces subtilisin inhibitor) at 2·6 Å resolution

1979 • 83 citations

Probing the mechanism and improving the rate of substrate-assisted catalysis in subtilisin BPN'

1991 • 69 citations

Active-site mapping of bovine and human blood coagulation serine proteases using synthetic peptide 4-nitroanilide and thio ester substrates

1984 • 55 citations

Fusion proteins in biotechnology and structural biology

1992 • 34 citations

Site-Specific Proteolysis of Fusion Proteins

1990 • 32 citations