Induction of Phosphodiesterases 3B, 4A4, 4D1, 4D2, and 4D3 in Jurkat T-cells and in Human Peripheral Blood T-lymphocytes by 8-Bromo-cAMP and Gs-coupled Receptor Agonists

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

β-Arrestin: a Protein that Regulates β-adrenergic Receptor Function

1990 • 1,162 citations

T cells and eosinophils in the pathogenesis of asthma

1992 • 585 citations

Protein kinases that phosphorylate activated G protein‐coupled receptors

1995 • 520 citations

Cyclooxygenase is an immediate-early gene induced by interleukin-1 in human endothelial cells.

1990 • 484 citations

Molecular mechanisms of membrane receptor desensitization

1993 • 442 citations

Phosphorylation and Activation of a cAMP-specific Phosphodiesterase by the cAMP-dependent Protein Kinase

1996 • 406 citations

Phosphodiesterase inhibitors: new opportunities for the treatment of asthma.

1991 • 383 citations

Recent Progress in Understanding the Hormonal Regulation of Phosphodiesterases

1995 • 313 citations

A Family of Human Phosphodiesterases Homologous to the Dunce Learning and Memory Gene Product of Drosophila melanogaster are Potential Targets for Antidepressant Drugs

1993 • 287 citations

Two distinct pathways for cAMP-mediated down-regulation of the β2-adrenergic receptor

1989 • 271 citations

Isolation and characterization of a previously undetected human cAMP phosphodiesterase by complementation of cAMP phosphodiesterase-deficient Saccharomyces cerevisiae

1993 • 219 citations

Identification of cyclic AMP phosphodiesterases 3, 4 and 7 in human CD4⁺ and CD8⁺ T‐lymphocytes: role in regulating proliferation and the biosynthesis of interleukin‐2

1996 • 218 citations

Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase.

1989 • 192 citations

Characterization of five different proteins produced by alternatively spliced mRNAs from the human cAMP-specific phosphodiesterase PDE4D gene

1997 • 186 citations

The short-term activation of a rolipram-sensitive, cAMP-specific phosphodiesterase by thyroid-stimulating hormone in thyroid FRTL-5 cells is mediated by a cAMP-dependent phosphorylation.

1994 • 183 citations

Molecular cloning of rat homologues of the Drosophila melanogaster dunce cAMP phosphodiesterase: evidence for a family of genes.

1989 • 171 citations

Effect of eight weeks of treatment with salmeterol on bronchoalveolar lavage inflammatory indices in asthmatics.

1994 • 160 citations

Molecular cloning and expression of human myocardial cGMP-inhibited cAMP phosphodiesterase.

1992 • 158 citations

Cytokine induction of cytosolic phospholipase A2 and cyclooxygenase-2 mRNA is suppressed by glucocorticoids in human epithelial cells

1996 • 153 citations

Cloning and characterization of mammalian homologs of the Drosophila dunce+ gene.

1989 • 147 citations

The mRNA encoding a high-affinity cAMP phosphodiesterase is regulated by hormones and cAMP.

1989 • 142 citations

Expression and regulation of human and rat phosphodiesterase type IV isogenes

1994 • 139 citations

Cloning and expression of cDNA for a human low-Km, rolipram-sensitive cyclic AMP phosphodiesterase.

1990 • 133 citations

The ratPDE3/IVd phosphodiesterase gene codes for multiple proteins differentially activated by cAMP-dependent protein kinase

1994 • 132 citations

A low-Km, rolipram-sensitive, cAMP-specific phosphodiesterase from human brain. Cloning and expression of cDNA, biochemical characterization of recombinant protein, and tissue distribution of mRNA.

1993 • 130 citations

Properties and hormonal regulation of two structurally related cAMP phosphodiesterases from the rat Sertoli cell.

1991 • 130 citations

Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants

1996 • 128 citations

Activation and selective inhibition of a cyclic AMP-specific phosphodiesterase, PDE-4D3.

1995 • 127 citations

Stimulation of beta adrenoceptors in a human monocyte cell line (U937) up-regulates cyclic AMP-specific phosphodiesterase activity.

1992 • 124 citations

Hormone-sensitive cyclic GMP-inhibited cyclic AMP phosphodiesterase in rat adipocytes. Regulation of insulin- and cAMP-dependent activation by phosphorylation

1991 • 119 citations

Regulation of distinct cyclic AMP-specific phosphodiesterase (phosphodiesterase type 4) isozymes in human monocytic cells.

1995 • 118 citations

Isobutylmethylxanthine stimulates adenylate cyclase by blocking the inhibitory regulatory protein, Gi.

1988 • 99 citations

Could isoenzyme-selective phosphodiesterase inhibitors render bronchodilator therapy redundant in the treatment of bronchial asthma?

1992 • 97 citations

Salbutamol Up-regulates PDE4 Activity and Induces a Heterologous Desensitization of U937 Cells to Prostaglandin E2

1995 • 92 citations

Regular albuterol, nedocromil sodium, and bronchial inflammation in asthma.

1995 • 92 citations

Structure of two rat genes coding for closely related rolipram-sensitive cAMP phosphodiesterases. Multiple mRNA variants originate from alternative splicing and multiple start sites.

1994 • 92 citations

Prolonged beta adrenoceptor stimulation up-regulates cAMP phosphodiesterase activity in human monocytes by increasing mRNA and protein for phosphodiesterases 4A and 4B.

1996 • 85 citations

Cyclic nucleotide phosphodiesterases from purified human CD4⁺ and CD8⁺ T lymphocytes

1995 • 79 citations

Activation of the particulate low Km phosphodiesterase of adipocytes by addition of cAMP-dependent protein kinase.

1988 • 78 citations

The cDNA of a human lymphocyte cyclic-AMP phosphodiesterase (PDE IV) reveals a multigene family

1993 • 75 citations

Molecular cloning and functional expression in yeast of a human cAMP‐specific phosphodiesterase subtype (PDE IV‐C)

1995 • 74 citations

Phosphodiesterase 4 and tolerance to beta 2-adrenoceptor agonists in asthma.

1996 • 73 citations

Phosphodiesterase 4 and tolerance to β2-adrenoceptor agonists in asthma

1996 • 72 citations

THE BETA-AGONIST CONTROVERSY

1996 • 71 citations

Calcium mobilization in Jurkat cells via A_2b adenosine receptors

1997 • 70 citations

Characterization of the cDNA and Gene Encoding Human PDE3B, the cGIP1 Isoform of the Human Cyclic GMP-Inhibited Cyclic Nucleotide Phosphodiesterase Family

1996 • 67 citations

Cyclic AMP-dependent Regulation of Lipid Mediators in White Cells: A Unifying Concept for Explaining the Efficacy of Theophylline in Asthma

1987 • 57 citations

A New Cyclic Nucleotide Phosphodiesterase Isozyme Expressed in the T-Lymphocyte Cell Lines

1993 • 52 citations

A mouse homolog of dunce, a gene important for learning and memory in drosophila, is preferentially expressed in olfactory receptor neurons

1995 • 50 citations

Isolation of a cDNA encoding a human rolipram-sensitive cyclic AMP phosphodiesterase (PDE IVD)

1994 • 46 citations

Molecular cloninc and expression, in both COS-1 cells and S. cerevisiae, of a human cytosolic type-IVA cyclic AMP specific phosphodiesterase (hPDE-IVA-h6.1)

1994 • 46 citations

Identification of cyclic AMP‐phosphodiesterase variants from the PDE4D gene expressed in human peripheral mononuclear cells

1996 • 40 citations

Modulation of cellular responses by hormones: role of cAMP specific, rolipram-sensitive phosphodiesterases

1994 • 32 citations

Phosphodiesterase IV inhibitors as therapy for eosinophil-induced lung injury in asthma.

1994 • 31 citations

Protein kinase A regulation of cAMP phosphodiesterase expression in rat skeletal myoblasts.

1994 • 29 citations

Selective Phosphodiesterase Inhibitors in the Therapy of Asthma

1995 • 26 citations

Identification of Phosphodiesterase IV Activity and Its Cyclic Adenosine Monophosphate-Dependent Up-Regulation in a Human Keratinocyte Cell Line (HaCaT)

1995 • 25 citations

Methylxanthines and Phosphodiesterase Inhibitors in the Treatment of Airways Disease

1995 • 17 citations

Isoprenaline induction of cAMP-phosphodiesterase in guinea-pig macrophages occurs in the presence, but not in the absence, of the phosphodiesterase type IV inhibitor rolipram

1995 • 17 citations