Freeze-fracture cytochemistry: a simplified guide and update on developments

Synaptic vesicle exocytosis captured by quick freezing and correlated with quantal transmitter release.

1979 • 1,408 citations

Colloidal Gold: Principles, Methods, and Applications

2012 • 1,167 citations

Freeze-Etching Nomenclature

1975 • 1,141 citations

Cell membrane receptors for drugs and hormones: A multidisciplinary approach

1979 • 850 citations

Organization of acetylcholine receptors in quick-frozen, deep-etched, and rotary-replicated Torpedo postsynaptic membrane.

1979 • 429 citations

Chemical Characterization and Surface Orientation of the Major Glycoprotein of the Human Erythrocyte Membrane

1972 • 401 citations

Principles and Techniques of Electron Microscopy: Biological Applications

1977 • 397 citations

Immunolabelling for electron microscopy

1983 • 335 citations

Techniques in immunocytochemistry

1983 • 298 citations

Freeze-etch localization of concanavalin A receptors to the membrane intercalated particles of human erythrocyte ghost membranes

1974 • 246 citations

Localization of A Antigen Sites on Human Erythrocyte Ghosts

1971 • 225 citations

Procedure for freeze-drying molecules adsorbed to mica flakes

1983 • 222 citations

New instruments which facilitate rapid freezing at 83 K and 6 K

1982 • 211 citations

Freeze Fracture: Methods, Artifacts, and Interpretations

1979 • 210 citations

Detection of microdomains in biomembranes An appraisal of recent developments in freeze-fracture cytochemistry

1983 • 181 citations

Immunolocalization of MP70 in lens fiber 16-17-nm intercellular junctions.

1987 • 124 citations

Label-fracture: a method for high resolution labeling of cell surfaces.

1984 • 120 citations

Electron Microscopy Of Subcellular Dynamics

1989 • 119 citations

Anionic sites on the membrane intercalated particles of human erythrocyte ghost membranes. Freeze-etch localization

1973 • 116 citations

Appearance and distribution of surface proteins of the human erythrocyte membrane. An electron microscope and immunochemical labeling study.

1978 • 97 citations

Incorporation and turnover of biotin-labeled actin microinjected into fibroblastic cells: an immunoelectron microscopic study.

1989 • 91 citations

Cells and tissues: A three-dimensional approach by modern techniques in microscopy

1990 • 81 citations

Freeze-Fracture Cytochemistry: Replicas of Critical Point-Dried Cells and Tissues After Fracture-Label

1981 • 80 citations

Cytoskeletal reorganization of human platelets after stimulation revealed by the quick-freeze deep-etch technique.

1987 • 69 citations

Freeze-fracture cytochemistry: localization of wheat-germ agglutinin and concanavalin A binding sites on freeze-fractured pancreatic cells.

1981 • 67 citations

Freeze-fracture cytochemistry: thin sections of cells and tissues after labeling of fractures faces.

1981 • 62 citations

Regionalization of transmembrane glycoproteins in the plasma membrane of boar sperm head is revealed by fracture-label.

1983 • 58 citations

Fracture-label:O cytochemistry of freeze-fracture faces in the erythrocyte membrane.

1981 • 58 citations

Filipin as a cholesterol probe. I. Morphology of filipin-cholesterol interaction in lipid model systems.

1984 • 57 citations

Preferential association of glycoproteins to the euchromatin regions of cross-fractured nuclei is revealed by fracture-label.

1986 • 56 citations

Free diffusion to and from the inner nuclear membrane of newly synthesized plasma membrane glycoproteins.

1987 • 51 citations

Colloidal gold as a tool in molecular biology

1983 • 45 citations

Fracture-Flip: New High-Resolution Images Of Cell Surfaces After Carbon Stabilization Of Freeze-Fractured Membranes

1988 • 42 citations

Protein‐A gold particles as markers in replica immunocytochemistry: high resolution electron microscope investigations of plasma membrane surfaces

1982 • 42 citations

Cell surface nanoanatomy of Leishmania major as revealed by fracture-flip. A surface meshwork of 44 nm fusiform filaments identifies infective developmental stage promastigotes.

1989 • 42 citations

Analysis of membrane structure in the transitional epithelium of rat urinary bladder. 3. Localization of cholesterol using filipin and digitonin

1981 • 41 citations

Postreplication labeling of E‐leaflet molecules: Membrane immunoglobulins localized in sectioned, labeled replicas examined by TEM and HVEM

1987 • 40 citations

Introduction of a high-resolution cytochemical method for studying the distribution of phospholipids in biological tissues.

1988 • 39 citations

Labelled‐replica techniques: post‐shadow labelling of intramembrane particles in freeze‐fracture replicas

1982 • 39 citations

Immunocytochemical study of the partition and distribution of Sindbis virus glycoproteins in freeze-fractured membranes of infected baby hamster kidney cells.

1985 • 39 citations

Freeze-fracture cytochemistry: partition of glycophorin in freeze-fractured human erythrocyte membranes.

1982 • 38 citations

Cell surface labelling with gold colloid particulates: the use of avidin and staphylococcal protein A‐coated gold in conjunction with biotin and fc‐bearing ligands

1981 • 38 citations

A correlative study on the topographical distribution of the receptors for low density lipoprotein (LDL) conjugated to colloid gold in cultured human skin fibroblasts employing thin section, freeze-fracture, deep-etching, and surface replication techniques.

1982 • 37 citations

The cell surface of Trypanosoma cruzi: a fracture-flip, replica-staining label-fracture survey.

1989 • 36 citations

Restriction of glycolipids to the outer half of a plasma membrane: Concanavalin A labeling of membrane halves in acanthamoeba castellanii

1983 • 34 citations

Compartmentalization of intracellular membrane glycocomponents is revealed by fracture-label.

1984 • 33 citations

Membrane assembly in retinal photoreceptors. II. Immunocytochemical analysis of freeze-fractured rod photoreceptor membranes using anti- opsin antibodies

1985 • 31 citations

Freeze-fracture studies of membranes

1991 • 31 citations

Molecular demarcation of surface domains as established by label-fracture cytochemistry of boar spermatozoa.

1987 • 29 citations

The acrosomal membrane of boar sperm: a Golgi-derived membrane poor in glycoconjugates.

1985 • 28 citations

Freeze‐fracture cytochemistry: Review of methods

1989 • 28 citations

Labeling of structural elements at the ventral plasma membrane of fibroblasts with the immunogold technique.

1987 • 27 citations

Vitronectin at sites of cell-substrate contact in cultures of rat myotubes.

1986 • 27 citations

Label-fracture of cell surfaces by replica staining.

1988 • 26 citations

Macromolecular dynamics of the cell surface during the formation of coated pits is revealed by fracture-flip

1988 • 22 citations

T-lymphocyte heterogeneity: wheat germ agglutinin labeling of transmembrane glycoproteins.

1982 • 22 citations

Immunocytochemical demonstrations of cytoplasmic and cell-surface EGF receptors in A431 cells using cryo-ultramicrotomy, surface replication, freeze-etching and label fracture

1985 • 21 citations

High density of transmembrane glycoproteins on the flagellar surface of boar sperm cells.

1984 • 21 citations

Detection of surface-bound ligands by freeze-fracture autoradiography.

1985 • 18 citations

Distribution of myosin and relationship to actin organization in cortical and subcortical areas of antibody-labelled, quick-frozen, deep-etched fibroblast cytoskeletons

1987 • 18 citations

Strategy and tactics in electron microscopy of cell surfaces

1989 • 17 citations

A Guide to Fracture Label: Cytochemical Labeling of Freeze-Fractured Cells

1986 • 16 citations

Surface views of nuclear pores in isolated rat liver nuclei as revealed by fracture-flip/Triton-X.

1989 • 14 citations

Capping of HLA antigens in human lymphocytes as followed by immunogold label-fracture.

1989 • 14 citations

High-resolution surface views of human lymphocytes during capping of CD4 and HLA antigens as revealed by immunogold fracture-flip

1990 • 14 citations

Molecular cytochemistry of CD3 and CD4 antigens in human lymphocytes as studied by label-fracture and by fracture-label

1989 • 14 citations

Use of peldri II as a sublimation dehydrant in place of critical‐point drying in fracture‐label cytochemistry and in backscattered electron imaging fracture‐label

1990 • 13 citations

Polypeptides of the chloroplast envelope membranes as visualized by immunochemical techniques.

1986 • 12 citations

Demonstration of antigens at both sides of plasma membranes in one coincident electron microscopic image: a double-immunogold replica study of virus-infected cells.

1988 • 12 citations

Structural and cytochemical differentiation of membrane elements of the apical membrane of amphibian urinary bladder epithelial cells. A label fracture study

1985 • 12 citations

Direct visualization of the interrelationship between intramembrane and extracellular structures.

1989 • 11 citations

FREEZE‐FRACTURE, LABELLED‐REPLICA, AND ELECTROPHYSIOLOGICAL STUDIES OF JUNCTIONAL FOLD DESTRUCTION IN MY ASTHENIA GRAVIS AND EXPERIMENTAL AUTOIMMUNE MYSTHENIA GRAVIS*

1981 • 11 citations

Interactions of lectins with specific cell types in toad urinary bladder. Surface distribution revealed by colloidal gold probes and label fracture.

1986 • 11 citations

Topographical and planar distribution of Helix pomatia lectin‐binding glycoconjugates in secretory granules and plasma membrane of pancreatic exocrine acinar cells of the rat: Demonstration of membrane heterogeneity

1989 • 11 citations

Backscattered electron imaging of lectin binding sites in tissues following freeze‐fracture cytochemistry

1988 • 10 citations

Immunogold Labelling of Cryosections and Cryofractures

1987 • 10 citations

Label-Fracture Cytochemistry

1989 • 9 citations

Monolayer freeze‐fracture autoradiography: origins and directions

1982 • 9 citations

Surface views of spermatozoa as revealed by fracture-flip

1989 • 9 citations

Fracture—Permeation: A technique to assess cytoplasm compactness after glutaraldehyde‐fixation

1986 • 9 citations

Colloidal Gold Immunoreplica Method

1989 • 8 citations

Ultrastructural patterns of ferritin permeation into glutaraldehyde‐fixed freeze‐fractured sarcomeres characterize stages of contraction in striated muscle

1986 • 8 citations

Ultrastructural and chemical analysis of the outer membrane leaflet of the human red blood cell.

1983 • 8 citations

Localization of light-harvesting complex II to the occluded surfaces of photosynthetic membranes.

1989 • 7 citations

An improved method for freeze-fracture radioautography of tissues and cells, as applied to duodenal epithelium and thymic lymphocytes.

1984 • 6 citations

Gold-Labeled Concanavalin A Binding Sites at the Cell Surface of Two Phycobionts Visualized by Deep-Etching

1982 • 5 citations

Membrane cytochemistry of the atrial peptide secretory pathway

1989 • 5 citations

Composite replicas: Methodologies for direct evaluation of the relationship between intramembrane and extramembrane structures

1989 • 5 citations

New techniques for the ultrastructural identification of human skeletal muscle fibre types

1987 • 2 citations

Filipin as a cholesterol probe

1984 • 2 citations

HVEM and conventional TEM studies of post-shadow labeled surface molecules in freeze-etch replicas

1986 • 2 citations

Immunolabelling of freeze-fractured tissue: thin sections of rat aorta after labelling of elastic laminae.

1987 • 2 citations