Vam2/Vps41p and Vam6/Vps39p Are Components of a Protein Complex on the Vacuolar Membranes and Involved in the Vacuolar Assembly in the Yeast Saccharomyces cerevisiae

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Green Fluorescent Protein as a Marker for Gene Expression

1994 • 6,572 citations

[12] Unidirectional digestion with exonuclease III in DNA sequence analysis

1987 • 898 citations

Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants.

1992 • 832 citations

The fungal vacuole: composition, function, and biogenesis

1990 • 758 citations

[35] Epitope tagging and protein surveillance

1991 • 526 citations

Isolation and characterization of yeast mutants in the cytoplasm to vacuole protein targeting pathway.

1995 • 493 citations

PROTEINASE MUTANTS OF SACCHAROMYCES CEREVISIAE

1977 • 460 citations

Applications of high efficiency lithium acetate transformation of intact yeast cells using single‐stranded nucleic acids as carrier

1991 • 433 citations

Biochemistry and Function of Vacuoles

1978 • 400 citations

Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers

1990 • 376 citations

Green‐fluorescent protein as a new vital marker in plant cells

1995 • 371 citations

Sec6, Sec8, and Sec15 are components of a multisubunit complex which localizes to small bud tips in Saccharomyces cerevisiae.

1995 • 310 citations

Changes induced in the permeability barrier of the yeast plasma membrane by cupric ion

1988 • 283 citations

Endocytosis in yeast: Several of the yeast secretory mutants are defective in endocytosis

1985 • 280 citations

Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae.

1989 • 258 citations

Endocytosis in yeast: Evidence for the involvement of a small GTP-binding protein (Ypt7p)

1992 • 235 citations

Multiple methods of visualizing the yeast vacuole permit evaluation of its morphology and inheritance during the cell cycle.

1987 • 224 citations

Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae. I. Isolation and characterization of two classes of vam mutants.

1992 • 188 citations

The SLP1 gene of Saccharomyces cerevisiae is essential for vacuolar morphogenesis and function.

1990 • 123 citations

Sec8p and Sec15p are components of a plasma membrane-associated 19.5S particle that may function downstream of Sec4p to control exocytosis.

1992 • 114 citations

Biogenesis of the Vacuole in Saccharomyces cerevisiae

1992 • 108 citations

The VPS16 gene product associates with a sedimentable protein complex and is essential for vacuolar protein sorting in yeast.

1993 • 103 citations

Nucleotide sequence ofAMS1, the structure gene of vacuolarα-mannosidase of Saccharomyces cerevisiae

1989 • 102 citations

Vacuolar ion channel of the yeast, Saccharomyces cerevisiae.

1987 • 87 citations

Structure and function of the yeast vacuolar membrane proton ATPase

1989 • 79 citations

Characterization of the END1 gene required for vacuole biogenesis and gluconeogenic growth of budding yeast.

1989 • 72 citations

Mutants of Saccharomyces cerevisiae with defective vacuolar function

1988 • 72 citations

Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae. II. VAM7, a gene for regulating morphogenic assembly of the vacuoles.

1992 • 53 citations

Chemiosmotic coupling of ion transport in the yeast vacuole: Its role in acidification inside organelles

1994 • 27 citations

Mutational analysis of Vam4/Ypt7p function in the vacuolar biogenesis and morphogenesis in the yeast,Saccharomyces cerevisiae

1996 • 16 citations