Can calmodulin function without binding calcium?

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Molecular cloning: A laboratory manual

1990 • 85,659 citations

Experiments in molecular genetics

1972 • 28,260 citations

Methods in yeast genetics

1979 • 7,824 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

Methods in Yeast Genetics

1988 • 4,905 citations

DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.

1987 • 2,266 citations

Fluorographic detection of radioactivity in polyacrylamide gols with the water-soluble fluor, sodium salicylate

1979 • 1,976 citations

[10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics

1987 • 1,375 citations

Measurement of protein-binding phenomena by gel filtration

1962 • 1,275 citations

Structure of calmodulin refined at 2.2 Å resolution

1988 • 1,019 citations

A hyper-recombination mutation in S. cerevisiae identifies a novel eukaryotic topoisomerase

1989 • 532 citations

Energetics of Ligand Binding to Proteins

1975 • 519 citations

Structural similarities between the Ca2+-dependent regulatory proteins of 3':5'-cyclic nucleotide phosphodiesterase and actomyosin ATPase.

1976 • 517 citations

Isolation of the yeast calmodulin gene: Calmodulin is an essential protein

1986 • 400 citations

Isolation, Characterization, and Expression in Escherichia coli of the DNA Polymerase Gene from Thermus aquaticus

1989 • 370 citations

Effects of cations on affinity of calmodulin for calcium: ordered binding of calcium ions allows the specific activation of calmodulin-stimulated enzymes. Theoretical approach to study of multiple ligand binding to a macromolecule

1981 • 321 citations

Genetic analysis of the mitotic transmission of minichromosomes

1985 • 310 citations

IRA2, a second gene of Saccharomyces cerevisiae that encodes a protein with a domain homologous to mammalian ras GTPase-activating protein.

1990 • 305 citations

Yeast has homologs (CNA1 and CNA2 gene products) of mammalian calcineurin, a calmodulin-regulated phosphoprotein phosphatase.

1991 • 297 citations

Identification and characterization of the calmodulin-binding domain of neuromodulin, a neurospecific calmodulin-binding protein.

1988 • 262 citations

Specification of surface mating systems among conjugative drug resistance plasmids in Escherichia coli K-12

1980 • 257 citations

Calmodulin is required for cell-cycle progression during G1 and mitosis.

1989 • 248 citations

Mutations in the glucocorticoid receptor zinc finger region that distinguish interdigitated DNA binding and transcriptional enhancement activities.

1989 • 240 citations

Rapid and Efficient Site-Specific Mutagenesis without Phenotypic Selection

1989 • 233 citations

Essential role for induced Ca2+ influx followed by [Ca2+]i rise in maintaining viability of yeast cells late in the mating pheromone response pathway. A study of [Ca2+]i in single Saccharomyces cerevisiae cells with imaging of fura-2.

1990 • 227 citations

A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae

1988 • 221 citations

Active involvement of Ca2+ in mitotic progression of Swiss 3T3 fibroblasts.

1990 • 183 citations

Site-directed Mutation of the Trigger Calcium-binding Sites in Cardiac Troponin C

1989 • 175 citations

Analysis and in vivo disruption of the gene coding for calmodulin in Schizosaccharomyces pombe.

1987 • 169 citations

Genetic evidence that zinc is an essential co-factor in the DNA binding domain of GAL4 protein

1987 • 169 citations

Isolation of nonsense suppressor mutants in Pseudomonas

1976 • 153 citations

Calcium binding to complexes of calmodulin and calmodulin binding proteins

1985 • 152 citations

Characterization and expression of the unique calmodulin gene of Aspergillus nidulans.

1990 • 133 citations

Mutations in paramecium calmodulin indicate functional differences between the C-terminal and N-terminal lobes in vivo

1990 • 132 citations

Two zinc fingers of a yeast regulatory protein shown by genetic evidence to be essential for its function

1987 • 128 citations

Multiple Ca2+/calmodulin-dependent protein kinase genes in a unicellular eukaryote.

1991 • 123 citations

Characterization of the 110-kdalton actin-calmodulin-, and membrane-binding protein from microvilli of intestinal epithelial cells.

1983 • 121 citations

The 110-kD protein-calmodulin complex of the intestinal microvillus is an actin-activated MgATPase.

1987 • 120 citations

Restoration by Calmodulin of a Ca ²⁺ -Dependent K ⁺ Current Missing in a Mutant of Paramecium

1986 • 100 citations

Calmodulin-binding proteins in the nuclei of quiescent and proliferatively activated rat liver cells.

1990 • 98 citations

Calcium-independent stimulation of Bordetella pertussis adenylate cyclase by calmodulin

1982 • 96 citations

The effects of deletions in the central helix of calmodulin on enzyme activation and peptide binding

1989 • 96 citations

Nucleotide sequence of the Saccharomyces cerevisiae ADE3 gene encoding C1-tetrahydrofolate synthase.

1986 • 87 citations

Calcium and calmodulin-dependent phosphorylation of a 62 kd protein induces microtubule depolymerization in sea urchin mitotic apparatuses

1988 • 86 citations

Vertebrate and yeast calmodulin, despite significant sequence divergence, are functionally interchangeable.

1989 • 84 citations

Cell lysis by induction of cloned lambda lysis genes

1981 • 76 citations

Ca²⁺ion binding to porcine pancreatic phospholipase A₂ and its function in enzyme-lipid interaction

1978 • 60 citations

Purification and biochemical properties of calmodulin from Saccharomyces cerevisiae

1987 • 59 citations

Yeast Calmodulin: Structural and Functional Differences Compared with Vertebrate Calmodulin1

1987 • 59 citations

Identification of the products of bacteriophage P22 genes, including a new late gene

1980 • 58 citations

A mutant Paramecium with a defective calcium-dependent potassium conductance has an altered calmodulin: a nonlethal selective alteration in calmodulin regulation.

1987 • 51 citations

Calcium and the regulation of mitotic events

1986 • 43 citations

Saccharomyces cerevisiae protein kinase dependent on Ca2+ and calmodulin

1989 • 42 citations

Functional expression of chicken calmodulin in yeast

1989 • 41 citations

High-affinity calcium-binding proteins in escherichia coli

1985 • 35 citations

Effect of microinjectecd calcium‐calmodulin on mitosis in PtK2 cells

1987 • 34 citations

Calmodulin stabilization of kinetochore microtubule structure to the effect of nocodazole.

1988 • 28 citations

Avian pp60c-src is more active when expressed in yeast than in vertebrate fibroblasts.

1987 • 28 citations

Calmodulins with deletions in the central helix functionally replace the native protein in yeast cells.

1991 • 27 citations

Sarcoplasmic calcium-binding proteins in insect muscle Isolation and properties of locust calmodulin

1981 • 23 citations

Ca²⁺/calmodulin‐dependent protein kinase in Saccharomyces cerevisiae

1987 • 22 citations

Substitution at position 116 of Schizosaccharomyces pombe calmodulin decreases its stability under nitrogen starvation and results in a sporulation-deficient phenotype.

1989 • 22 citations

Facile and gentle method for quantitative lysis of Escherichia coli and Salmonella typhimurium

1984 • 12 citations