Initial steps in protein membrane insertion. Bacteriophage M13 procoat protein binds to the membrane surface by electrostatic interaction.

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA

1972 • 2,842 citations

Functional messenger RNAs are produced by SP6in vitrotranscription of cloned cDNAs

1984 • 1,533 citations

Multiple Mechanisms of Protein Insertion into and Across Membranes

1985 • 720 citations

Regulation of a membrane component required for protein secretion in escherichia coli

1982 • 356 citations

Nuclear location signals in polyoma virus large-T

1986 • 351 citations

The antifolding activity of SecB promotes the export of the E. coli maltose-binding protein

1988 • 326 citations

Sequence of the leader peptidase gene of Escherichia coli and the orientation of leader peptidase in the bacterial envelope.

1983 • 307 citations

A signal sequence receptor in the endoplasmic reticulum membrane

1987 • 291 citations

Mutations which alter the function of the signal sequence of the maltose binding protein of Escherichia coli

1980 • 268 citations

Phosphatidylglycerol is involved in protein translocation across Escherichia coli inner membranes

1988 • 263 citations

Role of positive charge on the amino-terminal region of the signal peptide in protein secretion across the membrane.

1982 • 234 citations

Effects of two sec genes on protein assembly into the plasma membrane of Escherichia coli.

1985 • 217 citations

Effects of the complete removal of basic amino acid residues from the signal peptide on secretion of lipoprotein in Escherichia coli.

1983 • 214 citations

SecA protein, a peripheral protein of the Escherichia coli plasma membrane, is essential for the functional binding and translocation of proOmpA.

1989 • 206 citations

Synthesis, assembly into the cytoplasmic membrane, and proteolytic processing of the precursor of coliphage M13 coat protein.

1980 • 200 citations

ProOmpA is stabilized for membrane translocation by either purified E. coli trigger factor or canine signal recognition particle

1988 • 196 citations

Net N-C charge imbalance may be important for signal sequence function in bacteria

1986 • 176 citations

The “trigger factor cycle” includes ribosomes, presecretory proteins, and the plasma membrane

1988 • 162 citations

Evidence for posttranslational translocation of β-lactamase across the bacterial inner membrane

1982 • 153 citations

The SecY membrane component of the bacterial protein export machinery: analysis by new electrophoretic methods for integral membrane proteins.

1985 • 129 citations

Alterations in the extracellular domain of M13 procoat protein make its membrane insertion dependent on secA and secY

1988 • 128 citations

[76] Reconstitution of membrane processes

1979 • 127 citations

M13 procoat inserts into liposomes in the absence of other membrane proteins.

1985 • 121 citations

Filamentous phage pre-coat is an integral membrane protein: Analysis by a new method of membrane preparation

1982 • 112 citations

Import of frog prepropeptide GLa into microsomes requires ATP but does not involve docking protein or ribosomes.

1987 • 111 citations

Protein structure by solid state nuclear magnetic resonance

1985 • 107 citations

The cytoplasmic carboxy terminus of M13 procoat is required for the membrane insertion of its central domain

1986 • 92 citations

Conserved residues of the leader peptide are essential for cleavage by leader peptidase.

1985 • 90 citations

31P nuclear magnetic resonance and freeze-fracture electron microscopy studies on Escherichia coli. I. Cytoplasmic membrane and total phospholipids

1980 • 87 citations

Role of the leader peptide of maltose-binding protein in two steps of the export process

1988 • 72 citations

Import of honeybee prepromelittin into the endoplasmic reticulum. Requirements for membrane insertion, processing, and sequestration.

1986 • 71 citations

Role of amino-terminal positive charge on signal peptide in staphylokinase export across the cytoplasmic membrane of Escherichia coli.

1987 • 69 citations

Bacteriophage M13 Procoat Protein Inserts Into the Plasma Membrane as a Loop Structure

1987 • 66 citations

Both hydrophobic domains of M13 procoat are required to initiate membrane insertion.

1986 • 65 citations

Reconstitution of rapid and asymmetric assembly of M13 procoat protein into liposomes which have bacterial leader peptidase.

1983 • 65 citations

The role of the positively charged N-terminus of the signal sequence of E. coli outer membrane protein PhoE in export

1989 • 44 citations

Two receptors act sequentially

1987 • 20 citations