Regulated overproduction of the GAL4 gene product greatly increases expression from galactose-inducible promoters on multi-copy expression vectors in yeast

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

1980 • 8,711 citations

[12] One-step gene disruption in yeast

1983 • 3,302 citations

Sterile host yeasts (SHY): A eukaryotic system of biological containment for recombinant DNA experiments

1979 • 967 citations

Specific DNA binding of GAL4, a positive regulatory protein of yeast

1985 • 767 citations

Structure of a yeast pheromone gene (MFα): A putative α-factor precursor contains four tandem copies of mature α-factor

1982 • 587 citations

A GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site.

1982 • 575 citations

Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.

1980 • 567 citations

Saccharomyces cerevisiae GAL1-GAL10 Divergent Promoter Region: Location and Function of the Upstream Activating Sequence UAS_G

1984 • 395 citations

The organization and transcription of the galactose gene cluster of Saccharomyces

1981 • 350 citations

Isolation of galactose-inducible DNA sequences from Saccharomyces cerevisiae by differential plaque filter hybridization

1979 • 295 citations

Isolation of the yeast regulatory gene GAL4 and analysis of its dosage effects on the galactose/melibiose regulon.

1982 • 280 citations

Specific protein binding to far upstream activating sequences in polymerase II promoters.

1985 • 246 citations

Experimental Manipulation of Gene Expression

1983 • 213 citations

Synthesis in yeast of a functional oxidation-resistant mutant of human α1-antitrypsin

1984 • 209 citations

Saccharomyces cerevisiae GAL1-GAL10 divergent promoter region: location and function of the upstream activating sequence UASG.

1984 • 209 citations

Transcriptional regulation of the yeast cytochrome c gene.

1979 • 196 citations

A GAL family of upstream activating sequences in yeast: roles in both induction and repression of transcription.

1986 • 162 citations

Regulation of expression of the galactose gene cluster in Saccharomyces cerevisiae

1983 • 131 citations

Disruption of regulatory gene GAL80 in Saccharomyces cerevisiae: effects on carbon-controlled regulation of the galactose/melibiose pathway genes.

1984 • 129 citations

Two major outer envelope glycoproteins of Epstein-Barr virus are encoded by the same gene

1985 • 121 citations

Constitutive synthesis of the GAL4 protein, a galactose pathway regulator in saccharomyces cerevisiae

1979 • 116 citations

Vectors for High-Level, Inducible Expression of Cloned Genes in Yeast

1983 • 114 citations

Interaction of super-repressible and dominant constitutive mutations for the synthesis of galactose pathway enzymes in Saccharomyces cerevisiae

1977 • 109 citations

Functional domains of the yeast regulatory protein GAL4.

1986 • 95 citations

Expression and secretion in yeast of a 400-kda envelope glycoprotein derived from epstein-barr virus

1987 • 75 citations

The relationship of regulatory proteins and DNase I hypersensitive sites in the yeastGAL1-10genes

1985 • 73 citations

Identification of two proteins encoded by the Saccharomyces cerevisiae GAL4 gene.

1984 • 46 citations

Promoter Elements, Regulatory Elements, and Chromatin Structure of the Yeast his3 Gene

1983 • 40 citations

A region flanking the GAL7 gene and a binding site for GAL4 protein as upstream activating sequences in yeast

1985 • 39 citations

Transcription of multiple copies of the yeast GAL7 gene is limited by specific factors in addition to GAL4

1987 • 38 citations