Differentiation of ovine brown adipocyte precursor cells in a chemically defined serum‐free medium

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

A simple, rapid, and sensitive DNA assay procedure

1980 • 5,006 citations

DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.

1987 • 2,266 citations

Thermogenic mechanisms in brown fat.

1984 • 1,593 citations

Rapid similarity searches of nucleic acid and protein data banks.

1983 • 1,577 citations

Promoting effect of glucocorticoids on the differentiation of human adipocyte precursor cells cultured in a chemically defined medium.

1989 • 623 citations

Participation of one isozyme of cytosolic glycerophosphate dehydrogenase in the adipose conversion of 3T3 cells.

1979 • 481 citations

Molecular approach to thermogenesis in brown adipose tissue: cDNA cloning of the mitochondrial uncoupling protein.

1985 • 241 citations

Expression of uncoupling protein mRNA in thermogenic or weakly thermogenic brown adipose tissue. Evidence for a rapid beta-adrenoreceptor-mediated and transcriptionally regulated step during activation of thermogenesis.

1986 • 238 citations

Circulation, Respiration, and Metabolism

1985 • 234 citations

Complete cDNA-derived amino acid sequence of rat brown fat uncoupling protein.

1986 • 230 citations

Alpha- and beta-adrenergic induction of the expression of the uncoupling protein thermogenin in brown adipocytes differentiated in culture.

1990 • 204 citations

Development of a chemically defined serum-free medium for differentiation of rat adipose precursor cells

1987 • 180 citations

Glucocorticoid regulation of adipocyte differentiation: hormonal triggering of the developmental program and induction of a differentiation-dependent gene.

1985 • 139 citations

Adipose conversion of 3T3-L1 cells in a serum-free culture system depends on epidermal growth factor, insulin-like growth factor I, corticosterone, and cyclic AMP.

1990 • 123 citations

Sequential changes in the expression of mitochondrial protein mRNA during the development of brown adipose tissue in bovine and ovine species. Sudden occurrence of uncoupling protein mRNA during embryogenesis and its disappearance after birth

1989 • 120 citations

Morphology of adipose cells in lambs at birth and during subsequent transition of brown to white adipose tissue in cold and in warm conditions

1972 • 115 citations

Noradrenaline controls the concentration of the uncoupling protein in brown adipose tissue

1984 • 111 citations

Hormonal regulation of the differentiation of rat adipocyte precursor cells in primary culture.

1987 • 95 citations

Induction of the Triglyceride Pathway Enzymes and of Lipolytic Enzymes during Differentiation in a 'Preadipocyte' Cell Line

1978 • 93 citations

Differential regulation of beta 1- and beta 2-adrenergic receptor protein and mRNA levels by glucocorticoids during 3T3-F442A adipose differentiation.

1990 • 92 citations

Dexamethasone regulates the beta-adrenergic receptor subtype expressed by 3T3 L1 preadipocytes and adipocytes.

1982 • 92 citations

The role of data in scientific progress

1985 • 87 citations

Growth and adipose differentiation of sheep preadipocyte fibroblasts in serum‐free medium

1983 • 83 citations

Brown adipocytes differentiated in vitro can express the gene for the uncoupling protein thermogenin: Effects of hypothyroidism and norepinephrine

1989 • 78 citations

Isolation of myoblastic, fibro-adipogenic, and fibroblastic clonal cell lines from a common precursor and study of their requirements for growth and differentiation

1981 • 77 citations

Synthesis of mitochondrial uncoupling protein in brown adipocytes differentiated in cell culture.

1990 • 73 citations

Characterization of mitochondrial-uncoupling protein in bovine fetus and newborn calf

1987 • 73 citations

The gene for rat uncoupling protein: Complete sequence, structure of primary transcript and evolutionary relationship between exons

1988 • 58 citations

Requirements for Triggering of Adipocyte Differentiation by Glucocorticoids and Indomethacin*

1987 • 55 citations

Norepinephrine‐induced synthesis of the uncoupling protein thermogenin (UCP) and its mitochondrial targeting in brown adipocytes differentiated in culture

1990 • 43 citations

Growth of preadipocyte cell lines and cell strains from rodents in serum-free hormone-supplemented medium

1984 • 38 citations

Expression of the mitochondrial uncoupling protein in brown adipocytes

1987 • 35 citations

Differentiation of rabbit adipocyte precursor cells in a serum-free medium

1989 • 26 citations

Adrenergic regulation of the uncoupling protein expression in foetal rat brown adipocytes in primary culture

1989 • 26 citations

Nucleotide sequence of a cDNA encoding bovine brown fat uncoupling protein. Homology with ADP binding site of ADP/ATP carrier

1989 • 21 citations

Biochemical Mechanisms of Thermogenesis

1985 • 9 citations