Location of the protease-inhibitory region of secretory leukocyte protease inhibitor.

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

The 3′-Terminal Sequence of Escherichia coli 16S Ribosomal RNA: Complementarity to Nonsense Triplets and Ribosome Binding Sites

1974 • 3,603 citations

DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.

1987 • 2,266 citations

A gas-liquid solid phase peptide and protein sequenator.

1981 • 2,238 citations

Protein Inhibitors of Proteinases

1980 • 2,220 citations

Mapping the extended substrate binding site of cathepsin G and human leukocyte elastase. Studies with peptide substrates related to the alpha 1-protease inhibitor reactive site.

1979 • 602 citations

A sensitive new substrate for chymotrypsin

1979 • 584 citations

Isolation, properties, and complete amino acid sequence of human secretory leukocyte protease inhibitor, a potent inhibitor of leukocyte elastase.

1986 • 477 citations

TRANSLATIONAL COUPLING DURING EXPRESSION OF THE TRYPTOPHAN OPERON OF ESCHERICHIA COLI

1980 • 384 citations

High mutation frequency in DNA transfected into mammalian cells.

1983 • 338 citations

Pulmonary Emphysema and Proteolysis 1986

1987 • 292 citations

[47] Oligonucleotide probes for the screening of recombinant DNA libraries

1987 • 218 citations

The 2.5 A X-ray crystal structure of the acid-stable proteinase inhibitor from human mucous secretions analysed in its complex with bovine alpha-chymotrypsin.

1988 • 216 citations

Synthesis in yeast of a functional oxidation-resistant mutant of human α1-antitrypsin

1984 • 209 citations

The acid‐stable proteinase inhibitor of human mucous secretions (HUSI‐I, antileukoprotease)

1986 • 178 citations

Nucleotide sequence of the gene ompA coding the outer membrane protein II^*of Escherichia coli K-12

1980 • 151 citations

Isolation and properties of recombinant DNA produced variants of human alpha 1-proteinase inhibitor.

1985 • 143 citations

Isolation and sequence of a human gene encoding a potent inhibitor of leukocyte proteases

1986 • 140 citations

The basic trypsin inhibitor of bovine pancreas IV. The linear sequence of the 58 amino acids

1965 • 134 citations

Role of mRNA translational efficiency in bovine growth hormone expression in Escherichia coli.

1984 • 131 citations

[68] Succinylation

1967 • 131 citations

Synthesis in E. coli of α1-antitrypsin variants of therapeutic potential for emphysema and thrombosis

1985 • 125 citations

Enzymic replacement of the arginyl by a lysyl residue in the reactive site of soybean trypsin inhibitor

1969 • 115 citations

Active site mapping of the serine proteases human leukocyte elastase, cathepsin G, porcine pancreatic elastase, rat mast cell proteases I and II, bovine chymotrypsin A.alpha., and Staphylococcus aureus protease V-8 using tripeptide thiobenzyl ester substrates

1984 • 91 citations

Re-evaluation of the Kinetic Constants of Previously Investigated Specific Substrates of α-Chymotrypsin¹

1955 • 66 citations

[16] Refolding of recombinant proteins

1990 • 62 citations

The active centers of Streptomyces griseus protease 3, α-chymotrypsin, and elastase: enzyme-substrate interactions close to the scissile bond

1976 • 46 citations

Kinetic Studies on the Interaction of α₁-Proteinase Inhibitor (Pittsburgh) with Trypsin-Like Serine Proteinases

1986 • 39 citations

Preparation of chemically ‘mutated’ aprotinin homologues by semisynthesis

1988 • 35 citations

Secretion of Active, Full– and Half–Length Human Secretory Leukocyte Protease Inhibitor by Sacchammyces Cerevisiae

1989 • 18 citations