Homogeneous functional insulin receptor from 3T3-L1 adipocytes. Purification using N alpha B1-(biotinyl-epsilon-aminocaproyl)insulin and avidin-sepharose.

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A simplified method for cyanogen bromide activation of agarose for affinity chromatography

1974 • 1,951 citations

Avidin

1975 • 1,425 citations

Use of Polyethylene Glycol to Separate Free and Antibody-Bound Peptide Hormones in Radioimmunoassays†

1971 • 1,316 citations

[42] Detection and quantification of phosphotyrosine in proteins

1983 • 1,184 citations

[8] End-group analysis using dansyl chloride

1972 • 801 citations

The interaction of wheat germ agglutinin with sialoglycoproteins. The role of sialic acid.

1979 • 602 citations

A simplified method of sample preparation for determination of tritium, carbon-14, or sulfur-35 in blood or tissue by liquid scintillation counting

1966 • 530 citations

Evidence for the involvement of vicinal sulfhydryl groups in insulin-activated hexose transport by 3T3-L1 adipocytes.

1985 • 512 citations

Insulin Receptor: Evidence That It Is a Protein Kinase

1983 • 413 citations

Tyrosine-specific protein kinase activity is associated with the purified insulin receptor.

1983 • 324 citations

Electrophoretic resolution of three major insulin receptor structures with unique subunit stoichiometries.

1980 • 312 citations

Purification of insulin receptor with full binding activity.

1983 • 273 citations

The structure of insulin receptor and its subunits. Evidence for multiple nonreduced forms and a 210,000 possible proreceptor.

1982 • 225 citations

Affinity Chromatography and Purification of the Insulin Receptor of Liver Cell Membranes

1972 • 219 citations

Receptor-mediated phosphorylation of the hepatic insulin receptor: Evidence that the M _r 95,000 receptor subunit is its own kinase

1983 • 213 citations

Role of glycosylation in the processing of newly translated insulin proreceptor in 3T3-L1 adipocytes.

1984 • 207 citations

Insulin receptor is an insulin-dependent tyrosine protein kinase: copurification of insulin-binding activity and protein kinase activity to homogeneity from human placenta.

1984 • 201 citations

Purification of the insulin receptor from human placenta by chromatography on immobilized wheat germ lectin and receptor antibody.

1980 • 187 citations

The use of bifunctional biotinyl compounds to determine the arrangement of subunits in avidin

1971 • 183 citations

Activation of pyruvate dehydrogenase by direct addition of insulin to an isolated plasma membrane/mitochondria mixture: Evidence for generation of insulin's second messenger in a subcellular system

1980 • 172 citations

The subunit structure of rat liver insulin receptor. Antibodies directed against the insulin-binding subunit.

1980 • 144 citations

The properties of subunits of avidin coupled to Sepharose

1973 • 131 citations

The insulin receptor protein kinase. Physicochemical requirements for activity.

1983 • 119 citations

Development of insulin responsiveness of the glucose transporter and the (Na+,K+)-adenosine triphosphatase during in vitro adipocyte differentiation.

1982 • 108 citations

An avidin monomer affinity column for the purification of biotin-containing enzymes

1979 • 103 citations

Insulin, chemistry, structure, and function of insulin and related hormones

1980 • 92 citations

Insulin-stimulated tyrosine protein kinase. Characterization and relation to the insulin receptor.

1984 • 85 citations

Putative mediators of insulin action: regulation of pyruvate dehydrogenase and adenylate cyclase activities.

1982 • 85 citations

Purification of human liver propionyl-CoA carboxylase by carbon tetrachloride extraction and monomeric avidin affinity chromatography

1980 • 78 citations

Regulation of insulin receptor metabolism. Differentiation-induced alteration of receptor synthesis and degradation.

1981 • 76 citations

Pyruvate carboxylase: Isolation of the biotin-containing tryptic peptide and the determination of its primary sequence

1977 • 72 citations

Characterization of purified insulin receptor subunits.

1984 • 68 citations

Insulin and growth factors stimulate the phosphorylation of a Mr-22000 protein in 3T3-L1 adipocytes

1983 • 67 citations

Insulin-stimulated tyrosine protein kinase. Characterization and relation to the insulin receptor.

1984 • 67 citations

Kinetics of insulin receptor transit to and removal from the plasma membrane.

1983 • 66 citations

Studies on the Insulin Mediator: II. Separation of Two Antagonistic Biologically Active Materials from Fraction II

1980 • 64 citations

Biotinylinsulins as potential tools for receptor studies

1977 • 58 citations

Acetyl coenzyme A carboxylase. Rapid purification of the chick liver enzyme and steady state kinetic analysis of the carboxylase-catalyzed reaction.

1982 • 55 citations

Induction of pyruvate carboxylase apoenzyme and holoenzyme in 3T3-L1 cells during differentiation

1980 • 53 citations

Purification of the adipocyte insulin receptor by immunoaffinity chromatography.

1980 • 49 citations

Bis(tert.-butyloxycarbonyl)insulin

1971 • 37 citations

Dissociation Between Plasma Triglyceride Concentration and Tissue Lipoprotein Lipase Deficiency in Insulin-deficient Rats

1980 • 37 citations

Binding of insulin to solubilized insulin receptor from human placenta. Evidence for a single class of noninteracting binding sites.

1983 • 35 citations

Direct comparison of the rates of internalization and degradation of covalent receptor-insulin complexes in 3T3-L1 adipocytes. Internalization of occupied receptors is not the rate-limiting step in receptor-hormone complex degradation.

1984 • 24 citations