Use of in vitro protein synthesis from polymerase chain reaction-generated templates to study interaction of Escherichia coli transcription factors with core RNA polymerase and for epitope mapping of monoclonal antibodies

Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia

1985 • 9,128 citations

A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots

1984 • 2,189 citations

Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose

1984 • 1,697 citations

Production of monoclonal antibodies: Strategy and tactics

1980 • 1,594 citations

STRUCTURE AND FUNCTION OF BACTERIAL SIGMA FACTORS

1988 • 990 citations

Factor Stimulating Transcription by RNA Polymerase

1969 • 983 citations

IN VITRO SYNTHESIS OF PROTEIN IN MICROBIAL SYSTEMS

1973 • 675 citations

ompT encodes the Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification

1988 • 673 citations

The htpR gene product of E. coli is a sigma factor for heat-shock promoters

1984 • 593 citations

Sigma factors fromE. coli, B. subtilis, phage SP01, and phage T4 are homologous proteins

1986 • 460 citations

GENETIC CONTROL OF NITROGEN ASSIMILATION IN BACTERIA

1982 • 449 citations

Function of a Bacterial Activator Protein That Binds to Transcriptional Enhancers

1989 • 440 citations

The heat shock response of E. coli is regulated by changes in the concentration of σ32

1987 • 414 citations

Products of nitrogen regulatory genes ntrA and ntrC of enteric bacteria activate glnA transcription in vitro: evidence that the ntrA product is a sigma factor.

1985 • 352 citations

Transcription of glnA by purified Escherichia coli components: core RNA polymerase and the products of glnF, glnG, and glnL.

1985 • 335 citations

Genomic Amplification with Transcript Sequencing

1988 • 324 citations

Access to a Messenger RNA Sequence or Its Protein Product Is Not Limited by Tissue or Species Specificity

1989 • 306 citations

Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions

1987 • 241 citations

Identification and characterization of recD, a gene affecting plasmid maintenance and recombination in Escherichia coli

1986 • 219 citations

The T7 phage gene 10 leader RNA, a ribosome-binding site that dramatically enhances the expression of foreign genes in Escherichia coli

1988 • 189 citations

Involvement of Pseudomonas putida RpoN sigma factor in regulation of various metabolic functions

1989 • 157 citations

Characterization of the Escherichia coli transcription factor .sigma.70: localization of a region involved in the interaction with core RNA polymerase

1989 • 144 citations

Involvement of the ntrA gene product in the anaerobic metabolism of Escherichia coli

1987 • 116 citations

The nucleotide sequence of the nitrogen-regulation genentrAofKlebsiella pneumoniaeand comparison with conserved features in bacterial RNA polymerase sigma factors

1985 • 105 citations

Histidine regulation in Salmonella typhimurium: an activator attenuator model of gene regulation.

1975 • 99 citations

Structure and function of the .sigma.-70 subunit of Escherichia coli RNA polymerase. Monoclonal antibodies: localization of epitopes by peptide mapping and effects on transcription

1988 • 80 citations

The nucleotide sequence of the sigma factor gene ntrA (rpoN) of Azotobacter vinelandii: Analysis of conserved sequences in NtrA proteins

1987 • 74 citations

Escherichia coli sigma 54 RNA polymerase recognizes Caulobacter crescentus flbG and flaN flagellar gene promoters in vitro

1989 • 69 citations

Cell-free coupled transcription-translation system for investigation of linear DNA segments.

1980 • 55 citations

DNA amplification-restricted transcription-translation: rapid analysis of rhesus rotavirus neutralization sites.

1990 • 46 citations

Studies of the role of the Escherichia coli heat shock regulatory protein sigma 32 by the use of monoclonal antibodies.

1987 • 41 citations

Regulation of Gene Expression

1994 • 27 citations

Isolation and characterization of transducing phage coding for sigma subunit of Escherichia coli RNA polymerase.

1979 • 20 citations

RNA polymerase sigma-related proteins in Escherichia coli: Detection by antibodies against a synthetic peptide

1987 • 14 citations

Analysis of ColE1 expression in vitro after chromosome fragmentation

1983 • 9 citations

Incomplete polypeptides are formed in vitro by premature chain termination

1985 • 4 citations