Localized mutagenesis defines regions of the Bacillus thuringiensis delta-endotoxin involved in toxicity and specificity.

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Insecticidal crystal proteins of Bacillus thuringiensis

1989 • 1,950 citations

Preparation and partial characterization of amino acid transporting brush border membrane vesicles from the larval midgut of the cabbage butterfly (Pieris brassicae)

1987 • 605 citations

Loops in Globular Proteins: A Novel Category of Secondary Structure

1986 • 533 citations

Colloid-osmotic lysis is a general feature of the mechanism of action of Bacillus thuringiensis δ-endotoxins with different insect specificity

1987 • 448 citations

Specificity of Bacillus thuringiensis delta-endotoxins is correlated with the presence of high-affinity binding sites in the brush border membrane of target insect midguts.

1988 • 444 citations

Receptors on the brush border membrane of the insect midgut as determinants of the specificity of Bacillus thuringiensis delta-endotoxins

1990 • 365 citations

Cloning and expression of the Bacillus thuringiensis crystal protein gene in Escherichia coli.

1981 • 355 citations

Mechanism of Insect Resistance to the Microbial Insecticide Bacillus thuringiensis

1990 • 353 citations

Specificity of Bacillus thuringiensisδ‐endotoxins

1989 • 351 citations

Specific-primer-directed DNA sequencing

1986 • 264 citations

Functional Analysis of Domains II, Ib, and III of Pseudomonas exotoxin

1989 • 193 citations

Two highly related insecticidal crystal proteins of Bacillus thuringiensis subsp. kurstaki possess different host range specificities

1989 • 182 citations

The toxicity of twoBacillus thuringiensis δ-endotoxins to gypsy moth larvae is inversely related to the affinity of binding sites on midgut brush border membranes for the toxins

1990 • 170 citations

Specificity of Bacillus thuringiensis var. colmeri insecticidal delta-endotoxin is determined by differential proteolytic processing of the protoxin by larval gut proteases

1986 • 154 citations

Location of the Bombyx mori specificity domain on a Bacillus thuringiensis delta-endotoxin protein.

1989 • 139 citations

On the Microassembly of Integral Membrane Proteins

1990 • 135 citations

Bacillus thuringiensis toxin inhibits K⁺‐gradient‐dependent amino acid transport across the brush border membrane of Pieris brassicae midgut cells

1986 • 133 citations

The hypervariable region in the genes coding for entomopathogenic crystal proteins of Bacillus thuringiensis: nucleotide sequence of the kurhd1 gene of subsp. kurstaki HD1

1986 • 101 citations

The measurement of insoluble proteins using a modified Bradford assay

1988 • 90 citations

Efficient transformation of Bacillus thuringiensis and B. cereus via electroporation: Transformation of acrystalliferous strains with a cloned delta-endotoxin gene

1989 • 86 citations

Specificity-determining regions of a lepidopteran-specific insecticidal protein produced by Bacillus thuringiensis.

1990 • 77 citations

Facile preparation and characterization of the toxin from Bacillus thuringiensis var. kurstaki

1989 • 72 citations

Models for the structure and function of the Bacillus thuringiensis δ-endotoxins determined by compilational analysis

1990 • 70 citations

Sequence of an operon containing a novel δ-endotoxin gene fromBacillus thuringiensis

1991 • 58 citations

Specificity of Bacillus thuringiensis for lepidopteran larvae: factors involved in vivo and in the structure of a purified protoxin

1989 • 50 citations

Functional mapping of an entomocidal δ-endotoxin

1989 • 46 citations

Barium and Calcium Block Bacillus Thuringiensis Subspecies Kurstaki δ-Endotoxin Inhibition of Potassium Current Across Isolated Midgut of Larval Manduca Sexta

1988 • 42 citations

The Bacillus thuringiensis delta-endotoxin. Evidence for a two domain structure of the minimal toxic fragment.

1990 • 40 citations

A Simple and Efficient Procedure for Generating Random Point Mutations and for Codon Replacements Using Mixed Oligodeoxynucleotides

1988 • 38 citations

Neither barium nor calcium prevents the inhibition by Bacillus thuringiensis δ‐endotoxin of sodium‐ or potassium gradient‐dependent amino acid accumulation by tobacco hornworm midgut brush border membrane vesicles

1989 • 36 citations

In vivo generation of hybrids between two Bacillus thuringiensis insect-toxin-encoding genes

1991 • 36 citations

Expression of Bacillus thuringiensis delta-endotoxin genes during vegetative growth

1990 • 35 citations

Characterization of the toxicity and cytopathic specificity of a cloned Bacillus thuringiensis crystal protein using insect cell culture

1987 • 31 citations

Two structural domains as a general fold of the toxic fragment of the Bacillus thuringiensisδ‐endotoxins

1991 • 31 citations

Genetics and Biotechnology of Bacilli

1990 • 20 citations