Fine Affinity Discrimination by Yeast Surface Display and Flow Cytometry

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Rapid evolution of a protein in vitro by DNA shuffling

1994 • 1,901 citations

Yeast surface display for screening combinatorial polypeptide libraries

1997 • 1,668 citations

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution.

1994 • 1,274 citations

Display of heterologous proteins on the surface of microorganisms: From the screening of combinatorial libraries to live recombinant vaccines

1997 • 511 citations

Antibody phage display technology and its applications

1998 • 506 citations

Bound water molecules and conformational stabilization help mediate an antigen-antibody association.

1994 • 471 citations

Isolation of High-affinity Monomeric Human Anti-c-erbB-2 Single chain Fv Using Affinity-driven Selection

1996 • 295 citations

Increasing the secretory capacity of Saccharomyces cerevisiae for production of single-chain antibody fragments

1998 • 255 citations

Strategies for selection of antibodies by phage display

1998 • 245 citations

Phage display of combinatorial antibody libraries

1997 • 223 citations

Antibody affinity maturation using bacterial surface display

1998 • 194 citations

Selection of functional T cell receptor mutants from a yeast surface-display library

1999 • 171 citations

Optimal Screening of Surface-Displayed Polypeptide Libraries

1998 • 140 citations

A Mutational Analysis of the Binding of Two Different Proteins to the Same Antibody

1996 • 131 citations

Isolation of anti-T cell receptor scFv mutants by yeast surface display

1997 • 130 citations

The Contribution of Contact and Non-contact Residues of Antibody in the Affinity of Binding to Antigen

1993 • 128 citations

Phage display: applications, innovations, and issues in phage and host biology

1998 • 98 citations

Phage display: applications, innovations, and issues in phage and host biology

1998 • 85 citations

Affinity maturation of the BR96 anti-carcinoma antibody by codon-based mutagenesis.

1995 • 64 citations

Engineering multiple properties of a protein by combinatorial mutagenesis.

1993 • 61 citations

The effect of water activity on the association constant and the enthalpy of reaction between lysozyme and the specific antibodies D1.3 and D44.1

1996 • 59 citations

Selective phage infection mediated by epitope expression on F pilus 1 1Edited by J. Karn

1997 • 42 citations

Review

1997 • 35 citations

Biosynthetic polypeptide libraries

1999 • 33 citations

Selection Procedures for Nonmatured Phage Antibodies: A Quantitative Comparison and Optimization Strategies

1995 • 32 citations

Thermodynamic characterization of affinity maturation: the D1.3 antibody and a higher-affinity mutant

1998 • 16 citations