Suppressors of the Ca(2+)-sensitive yeast mutant (csg2) identify genes involved in sphingolipid biosynthesis. Cloning and characterization of SCS1, a gene required for serine palmitoyltransferase activity.

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

A simple method for displaying the hydropathic character of a protein

1982 • 22,471 citations

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Methods in Yeast Genetics

1988 • 4,905 citations

A rapid boiling method for the preparation of bacterial plasmids

1981 • 3,334 citations

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

1984 • 2,401 citations

A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector

1987 • 1,181 citations

A rapid, efficient method for isolating DNA from yeast

1986 • 358 citations

Inositol Phosphorylceramide, a Novel Substance and the Chief Member of a Major Group of Yeast Sphingolipids Containing a Single Inositol Phosphate

1974 • 203 citations

Cloning and characterization of LCB1, a Saccharomyces gene required for biosynthesis of the long-chain base component of sphingolipids

1991 • 185 citations

Guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen of Saccharomyces cerevisiae

1993 • 183 citations

The phosphoinositol sphingolipids of Saccharomyces cerevisiae are highly localized in the plasma membrane

1991 • 173 citations

Identification of a glycolipid precursor of the Trypanosoma brucei variant surface glycoprotein.

1986 • 170 citations

Physical map of the Saccharomyces cerevisiae genome at 110-kilobase resolution.

1991 • 156 citations

The isolation and characterization of a mutant strain of Saccharomyces cerevisiae that requires a long chain base for growth and for synthesis of phosphosphingolipids.

1983 • 118 citations

Biosynthesis of phosphoinositol-containing sphingolipids from phosphatidylinositol by a membrane preparation from Saccharomyces cerevisiae

1980 • 116 citations

The contact site A glycoprotein of Dictyostelium discoideum carries a phospholipid anchor of a novel type.

1989 • 114 citations

A novel protein, CSG2p, is required for Ca2+ regulation in Saccharomyces cerevisiae.

1994 • 113 citations

Cloning and characterization of the Bacillus sphaericus genes controlling the bioconversion of pimelate into dethiobiotin

1990 • 102 citations

Sphingolipid long-chain-base auxotrophs of Saccharomyces cerevisiae: genetics, physiology, and a method for their selection

1992 • 100 citations

Biosynthesis of mannosylinositolphosphoceramide in Saccharomyces cerevisiae is dependent on genes controlling the flow of secretory vesicles from the endoplasmic reticulum to the Golgi.

1991 • 96 citations

ISOLATION AND PARTIAL CHARACTERIZATION OF A MAJOR INOSITOL-CONTAINING LIPID IN BAKER'S YEAST, MANNOSYL-DIINOSITOL, DIPHOSPHORYL-CERAMIDE

1969 • 90 citations

Characterization of enzymatic synthesis of sphingolipid long-chain bases in Saccharomyces cerevisiae: mutant strains exhibiting long-chain-base auxotrophy are deficient in serine palmitoyltransferase activity

1992 • 71 citations

The nucleotide sequence of the HEM1 gene and evidence for a precursor form of the mitochondrial 5-aminolevulinate synthase in Saccharomyces cerevisiae

1986 • 64 citations

2-Amino-3-ketobutyrate CoA ligase of Escherichia coli: stoichiometry of pyridoxal phosphate binding and location of the pyridoxyllysine peptide in the primary structure of the enzyme

1990 • 34 citations

Nucleotide sequence of the 2-amino-3-ketobutyrate coenzyme A ligase (kbl) gene ofE.coli

1988 • 31 citations