Purification of a human polyribosome-associated 3‘ to 5‘ exoribonuclease.

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids

1984 • 3,959 citations

Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: Results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes

1980 • 1,256 citations

Messenger RNA degradation in eukaryotes

1993 • 858 citations

Removal of poly(A) and consequent degradation of c-fos mRNA facilitated by 3′ AU-rich sequences

1988 • 691 citations

Cytoplasmic protein binds in vitro to a highly conserved sequence in the 5' untranslated region of ferritin heavy- and light-subunit mRNAs.

1988 • 684 citations

A turnover pathway for both stable and unstable mRNAs in yeast: evidence for a requirement for deadenylation.

1993 • 660 citations

3′-Terminal labelling of RNA with T4 RNA ligase

1978 • 556 citations

Two distinct destabilizing elements in the c-fos message trigger deadenylation as a first step in rapid mRNA decay.

1991 • 546 citations

A specific mRNA binding factor regulates the iron-dependent stability of cytoplasmic transferrin receptor mRNA

1989 • 511 citations

Deadenylation of the unstable mRNA encoded by the yeast MFA2 gene leads to decapping followed by 5'-->3' digestion of the transcript.

1994 • 511 citations

Polynucleotide phosphorylase and ribonuclease II are required for cell viability and mRNA turnover in Escherichia coli K-12.

1986 • 427 citations

Yeast cells lacking 5'-->3' exoribonuclease 1 contain mRNA species that are poly(A) deficient and partially lack the 5' cap structure.

1993 • 378 citations

The poly(A)-poly(A)-binding protein complex is a major determinant of mRNA stability in vitro.

1989 • 373 citations

Iron regulation of transferrin receptor mRNA levels requires iron-responsive elements and a rapid turnover determinant in the 3′ untranslated region of the mRNA.

1989 • 334 citations

Poly(A) shortening and degradation of the 3' A+U-rich sequences of human c-myc mRNA in a cell-free system.

1988 • 324 citations

Control of c-myc mRNA half-life in vitro by a protein capable of binding to a coding region stability determinant.

1992 • 301 citations

Histone mRNA concentrations are regulated at the level of transcription and mRNA degradation.

1983 • 298 citations

Evidence that the pathway of transferrin receptor mRNA degradation involves an endonucleolytic cleavage within the 3′ UTR and does not involve poly(A) tail shortening.

1994 • 290 citations

Mechanisms of mRNA decay in bacteria: a perspective

1988 • 290 citations

Regulation of Histone mRNA in the Unperturbed Cell Cycle: Evidence Suggesting Control at Two Posttranscriptional Steps

1991 • 224 citations

Translation initiation requires the PAB-dependent poly(A) ribonuclease in yeast

1992 • 212 citations

Multiple regulatory steps control histone mRNA concentrations

1988 • 199 citations

Regulation of histone mRNA in the unperturbed cell cycle: evidence suggesting control at two posttranscriptional steps.

1991 • 189 citations

Regulation of histone mRNA production and stability in serum-stimulated mouse 3T6 fibroblasts.

1983 • 186 citations

Cell-cycle regulation of histone gene expression

1986 • 183 citations

H4 histone messenger RNA decay in cell-free extracts initiates at or near the 3′ terminus and proceeds 3′ to 5′

1986 • 179 citations

The role of poly(A) in the translation and stability of mRNA

1990 • 154 citations

Mutations in the Yeast RNA14 and RNA15 Genes Result in an Abnormal mRNA Decay Rate; Sequence Analysis Reveals an RNA-Binding Domain in the RNA15 Protein

1991 • 153 citations

mRNA degradation by processive 3′-5′ exoribonucleases in Vitro and the implications for prokaryotic mRNA decay in Vivo

1991 • 149 citations

Seminal RNase: a unique member of the ribonuclease superfamily

1991 • 142 citations

3'-UTR-dependent deadenylation by the yeast poly(A) nuclease.

1992 • 140 citations

Purification and properties of a protein that binds to the C-terminal coding region of human c-myc mRNA.

1994 • 125 citations

Degradation of apolipoprotein II mRNA occurs via endonucleolytic cleavage at 5′-AAU-3′/5′-UAA-3′ elements in single-stranded loop domains of the 3′-noncoding region

1989 • 118 citations

Regulation of human histone gene expression: transcriptional and posttranscriptional control in the coupling of histone messenger RNA stability with DNA replication

1987 • 111 citations

Coupling of replication type histone mRNA levels to DNA synthesis requires the stem-loop sequence at the 3' end of the mRNA.

1987 • 110 citations

The increment of SII-facilitated transcript cleavage varies dramatically between elongation competent and incompetent RNA polymerase II ternary complexes

1993 • 106 citations

Identification of a 3'-->5' exonuclease activity associated with human RNA polymerase II.

1993 • 103 citations

In vitro deadenylation of mammalian mRNA by a HeLa cell 3′ exonuclease.

1991 • 102 citations

Properties of the exonuclease activity that degrades H4 histone mRNA.

1987 • 102 citations

Messenger RNA turnover in cell-free extracts

1990 • 101 citations

Ribonuclease multiplicity, diversity, and complexity

1993 • 99 citations

Sequence-specific endonucleolytic cleavage and protection of mRNA in Xenopus and Drosophila.

1993 • 99 citations

Properties of a HeLa cell 3' exonuclease specific for degrading poly(A) tails of mammalian mRNA.

1992 • 98 citations

Histone mRNA Degradation In Vivo: the First Detectable Step Occurs at or near the 3′ Terminus

1986 • 98 citations

The rate-limiting step in yeast PGK1 mRNA degradation is an endonucleolytic cleavage in the 3'-terminal part of the coding region.

1992 • 96 citations

The kinetics of mammalian gene expression

1991 • 91 citations

Thin-layer Chromatography

1967 • 90 citations

Processing of 9E3 mRNA and regulation of its stability in normal and Rous sarcoma virus-transformed cells.

1989 • 86 citations

Purification and characterization of Escherichia coli RNase I

1990 • 82 citations

Autogenous Regulation of Histone mRNA Decay by Histone Proteins in a Cell-Free System

1987 • 82 citations

Requirements for the Translational Repression of Ferritin Transcripts in Wheat Germ Extracts by a 90-kDa Protein from Rabbit Liver

1989 • 80 citations

Purification and properties of a nuclear exoribonuclease from Ehrlich ascites tumor cells.

1967 • 76 citations

E. coli RNases: Making sense of alphabet soup

1985 • 76 citations

Endonucleolysis in the turnover of insulin-like growth factor II mRNA.

1992 • 73 citations

Regulation of the mRNA for monocyte-derived neutrophil-activating peptide in differentiating HL60 promyelocytes.

1989 • 71 citations

Different complexes are formed on the 3′ end of histone mRNA with Nuclear and polyribosomal proteins

1991 • 64 citations

Removal of a 3′ non-coding sequence is an initial step in degradation of groα mRNA and is regulated by interleukin-1

1992 • 64 citations

Substrate specificity of the exonuclease activity that degrades H4 histone mRNA.

1987 • 58 citations

Site-specific cleavage of IGF-II mRNAs requires sequence elements from two distinct regions of the IGF-II gene

1992 • 51 citations

A 5′ exoribonuclease from cytoplasmic extracts of mouse sarcoma 180 ascites cells

1993 • 39 citations

Isolation and purification of double-stranded ribonuclease from calf thymus.

1977 • 37 citations

Isolation and characterization of two enzymatic activities from chick embryos which degrade double-stranded RNA.

1977 • 36 citations

A 5′–3′ exoribonuclease of human placental nuclei: purification and substrate specificity

1987 • 32 citations

Partial purification of a double-stranded RNA specific ribonuclease (RNAse D) from Krebs II ascites cells

1976 • 29 citations

Protection of mRNA against nucleases in cytoplasmic extracts of mouse sarcoma ascites cells

1993 • 22 citations

Purification and properties of a novel nucleolar exoribonuclease capable of degrading both single-stranded and double-stranded RNA

1985 • 20 citations

Individual purified core and linker histones induce histone H4 mRNA destabilization in vitro

1993 • 17 citations

Evolutionary conserved multiprotein complexes interact with the 3′ untranslated region of histone transcripts

1992 • 14 citations

Analysis of the multiple 5? and 3? termini of poly(A) + and poly(A)? deficient thymidylate synthase mRNA in growth-stimulated mouse fibroblasts

1989 • 7 citations