NUTRITIONAL REQUIREMENTS OF CELLS IN CULTURE, WITH SPECIAL REFERENCE TO NEURAL CELLS

Amino Acid Metabolism in Mammalian Cell Cultures

1959 • 4,025 citations

Nerve growth factor.

1968 • 2,025 citations

Nutrition Needs of Mammalian Cells in Tissue Culture

1955 • 1,562 citations

Nutrition of Animal Cells in Tissue Culture. I. Initial Studies on a Synthetic Medium.,

1950 • 1,066 citations

Relation of Oxygen and Temperature in the Preservation of Tissues by Refrigeration

1949 • 959 citations

Selective growth stimulating effects of mouse sarcoma on the sensory and sympathetic nervous system of the chick embryo

1951 • 947 citations

An improved nutrient solution for diploid Chinese hamster and human cell lines

1963 • 890 citations

Production of Malignancy in Vitro. IV. The Mouse Fibroblast Cultures and Changes Seen in the Living Cells

1943 • 843 citations

CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM

1965 • 833 citations

Culture of Normal Human Leukocytes

1967 • 774 citations

PURIFICATION OF A NERVE-GROWTH PROMOTING PROTEIN FROM THE MOUSE SALIVARY GLAND AND ITS NEURO-CYTOTOXIC ANTISERUM

1960 • 692 citations

The Maintenance of Human Normal Cells and Tumor Cells in Continuous Culture: I. Preliminary Report: Cultivation of Mesoblastic Tumors and Normal Tissue and Notes on Methods of Cultivation

1936 • 598 citations

The Growth in Vitro of Single Isolated Tissue Cells

1948 • 593 citations

Observations on the living developing nerve fiber

1906 • 573 citations

Growth, Nutrition, and Metabolism of Cells in Culture

1977 • 467 citations

The nerve growth factor: its mode of action on sensory and sympathetic nerve cells.

1966 • 456 citations

PRIMARY CULTURES OF DISSOCIATED SYMPATHETIC NEURONS

1973 • 426 citations

Essential role of the nerve growth factor in the survival and maintenance of dissociated sensory and sympathetic embryonic nerve cells in vitro

1963 • 417 citations

THE GROWTH AND MAINTENANCE OF TISSUE–CELL CULTURES IN FREE GAS EXCHANGE WITH THE ATMOSPHERE1

1963 • 417 citations

Replacement of serum by hormones permits growth of cells in a defined medium

1976 • 412 citations

Rapid Proliferation of Sublines of NCTC Clone 929 (Strain L) Mouse Cells in a Simple Chemically Defined Medium (MB 752/1)2

1959 • 327 citations

CELLS AND TISSUES IN CULTURE

1966 • 312 citations

A NERVE GROWTH-STIMULATING FACTOR ISOLATED FROM SNAKE VENOM

1956 • 309 citations

Fetuin, a New Globulin Isolated from Serum

1944 • 289 citations

Regulation of spontaneous activity and growth of embryonic chick heart cells in tissue culture

1967 • 280 citations

Implantation of tumors in the hind limb field of the embryonic chick and the developmental response of the lumbosacral nervous system

1948 • 263 citations

Stimulation of clonal growth of normal fibroblasts with substrata coated with basic polymers.

1976 • 254 citations

The effect of potassium on neuronal differentiation in cultures of dissociated newborn rat cerebellum

1972 • 244 citations

Selenium is an essential trace nutrient for growth of WI-38 diploid human fibroblasts.

1976 • 243 citations

Multiplication and division in mammalian cells

1976 • 242 citations

Induced differentiation of a neuroblastoma

1971 • 232 citations

Multiplication and Division in Mammalian Cells

1977 • 212 citations

Reversible dissociation of the mouse nerve growth factor protein into different subunits

1968 • 210 citations

Long-term cell culture of adult rat liver epithelial cells

1974 • 206 citations

Methods of Tissue Culture

1962 • 194 citations

Principles of Development

2018 • 187 citations

Fetal Bovine Serum: A Multivariate Standard

1975 • 182 citations

MOLECULAR GROWTH REQUIREMENTS OF SINGLE MAMMALIAN CELLS: THE ACTION OF FETUIN IN PROMOTING CELL ATTACHMENT TO GLASS

1958 • 181 citations

Osmolality of mammalian blood and of media for culture of mammalian cells

1970 • 175 citations

An improved chemically defined culture medium for strain L mouse cells based on growth responses to graded levels of nutrients including iron and zinc ions

1970 • 148 citations

THE MODE OF ACTION OF SOME PURGATIVE SALTS

1910 • 139 citations

Subunit structure and amino acid composition of mouse submaxillary gland nerve growth factor

1971 • 134 citations

Electrophysiologic study of cultured neurons dissociated from spinal cords and dorsal root ganglia of fetal mice

1973 • 127 citations

Nerve growth factor and its mode of action

1975 • 124 citations

Potassium concentration and number of neurons in cultures of dissociated ganglia

1970 • 124 citations

Amino acid sequences of mouse 2.5S nerve growth factor. II. Isolation and characterization of the thermolytic and peptic peptides and the complete covalent structure

1973 • 124 citations

Amino Acid Requirements of the Novikoff Hepatoma in vitro.

1959 • 116 citations

A Protein Growth Factor for Mammalian Cells in Culture

1958 • 116 citations

The potassium-sensitivity of isolated embryonic heart cells increases with development

1970 • 107 citations

Immunocytochemical localization of nerve growth factor (NGF) in the submandibular gland of adult mice by light and electron microscopy

1976 • 103 citations

The Adaptation of Additional Lines of NCTC Clone 929 (Strain L) Cells to Chemically Defined Protein-Free Medium NCTC 109<xref ref-type="fn" rid="FN1">2</xref>

1957 • 100 citations

Quality control studies on fetal bovine serum used in tissue culture

1971 • 94 citations

Effect of room fluorescent light on the deterioration of tissue culture medium

1976 • 93 citations

The Nutrition of Animal Tissues Cultivated <italic>In Vitro</italic>. I. A Survey of Natural Materials as Supplements to Synthetic Medium 199

1955 • 92 citations

The cultivation of tissues from chick embryos in solutions of NaCl, CaCl₂, KC1 and NaHCO₃

1911 • 84 citations

Glutamine metabolism by animal cells growing in a synthetic medium

1962 • 83 citations

AN IMPROVED CHEMICALLY DEFINED BASAL MEDIUM (CMRL-1415) FOR NEWLY EXPLANTED MOUSE EMBRYO CELLS

1966 • 81 citations

Neurons and glia in neural cultures

1975 • 80 citations

Nutritional requirements of primary cultures a neglected problem of modern biology

1974 • 79 citations

Albumin Replacement by Fatty Acids in Clonal Growth of Mammalian Cells

1963 • 75 citations

K + induced stimulation of oxygen uptake in cultured cerebral glial cells

1973 • 70 citations

Differentiation in vitro of Embryonic Cartilage and Bone in a Chemically-Defined Medium.

1965 • 69 citations

The cultivation of sympathetic nerves from the intestine of chick embryos in saline solutions

1912 • 68 citations

Evidence for a direct effect of temperature and thyroid hormone on myelinogenesis in vitro

1966 • 66 citations

Distinction between nerve growth factor and glial factor

1975 • 66 citations

Cell cultures of fetal rat brain

1973 • 66 citations

Relationships between glial and neuronal elements in the development of long term cultures of the spinal cord of the fetal mouse

1970 • 64 citations

An Autoclavable Powdered Culture Medium for Mammalian Cells

1968 • 64 citations

CULTIVATION OF MAMMALIAN CELLS IN DEFINED MEDIA WITH PROTEIN AND NONPROTEIN SUPPLEMENTS

1966 • 61 citations

Tissue cultures of cat cerebellum

1956 • 59 citations

Nonidentity of Fetuin and Protein Growth (Flattening) Factor

1959 • 58 citations

Effect of potassium on neuron survival in cultures of dissociated human nervous tissue

1971 • 58 citations

Effects of steroid hormones in fetal bovine serum on plating and cloning of human cells in vitro

1976 • 56 citations

Cultivation of Animal Cells in Chemically Defined Media, A Review

1973 • 55 citations

Nerve growth factor—Recent developments and perspectives

1976 • 48 citations

Critical adjustment of cysteine and glutamine concentrations for improved clonal growth of WI-38 cells

1977 • 47 citations

Construction and Use of Synthetic Media

1965 • 44 citations

A Chemically Defined Medium for Growth of Animal Cells in Suspension

1963 • 42 citations

Studies on the cultivation of mammalian cell lines in a serum-free, chemically defined medium

1973 • 40 citations

CONSTRUCTION OF TISSUE CULTURE MEDIA

1972 • 39 citations

Studies in vitro on single beating rat heart cells

1966 • 35 citations

A physico-chemical study of fetal calf sera used as tissue culture nutrient correlated with biological tests for toxicity

1967 • 35 citations

Chemosensitivity of mouse neuroblastoma cells in vitro

1973 • 34 citations

The cultivation of chick tissues in media of known chemical constitution

1912 • 33 citations

Oxygen and light effects on chromosomal aberrations in mouse cells in vitro

1977 • 32 citations

An improved heat‐stable glutamine‐free chemically defined medium for growth of mammalian cells

1971 • 32 citations

Phenylketonuria Metabolites in Cerebellum Culture Morphology

1967 • 30 citations

A Feeding Solution for Cultures of Human Fibroblasts

1933 • 30 citations

Growth ofChlamydia psittaci strain menigopneumonitis in mouse L cells cultivated in a defined medium in spinner cultures

1972 • 29 citations

Cell culture of the endocrine pancreas of the mouse in chemically defined media

1974 • 29 citations

REGULATION OF OXYGEN CONSUMPTION IN NEUROBLASTOMA CELLS: EFFECTS OF DIFFERENTIATION AND OF POTASSIUM

1973 • 28 citations

Transient inhibition of cell proliferation in rat glioma monolayer cultures by cortisol.

1976 • 28 citations

Chemically characterized concentrated corodies11Corody, pl. Corodies is defined as “an allowance as of food, provision, aliment, etc.”, here it is used in place of “nutrient media”. for continuous cell culture (the 7C's culture media)

1968 • 22 citations

“Feeding the Baby”-Designing the Culture Milieu to Enhance Cell Stability1

1974 • 21 citations

Effect of choline, high potassium, and low sodium on the number of neurons in cultures of dissociated chick ganglia

1971 • 21 citations

Heat-stable Chemically Defined Medium for Growth of Animal Cells in Suspension

1968 • 18 citations

The quantitative glucose and mineral nutrient requirements of mouse LS (suspension) cells in chemically defined medium

1971 • 15 citations

CULTIVATION OF NERVE TISSUE

1972 • 14 citations

Unique Requirements for Clonal Growth1

1974 • 14 citations

EFFECTS OF PYRIDOXINE DEFICIENCY IN NERVOUS TISSUE MAINTAINED IN VITRO

1969 • 13 citations

Somatic cells in vitro: their relationship to progenitive cells and to artificial milieux.

1967 • 13 citations

Inactivation of erythropoietin by Koshland's tryptophan reagent and by membrane filtration

1968 • 13 citations

An improved synthetic medium suitable for tissue culture of various mammalian cells.

1975 • 11 citations

Effect of osmotic pressure on neurogenesis in cultures of chick embryo spinal cords

1975 • 9 citations

The Serial Cultivation of Suspended BHK-21/13 Cells in Serum-Free Waymouth Medium

1976 • 8 citations

Heat-stable Chemically Defined Medium for Growth of Animal Cells in Suspension

1968 • 8 citations

Some <italic>In Vitro</italic> Requirements of Cells of C3H Mouse, Chinese Hamster, Green Monkey, and Human Origin

1966 • 7 citations

Maintenance of Fibroblasts in Artificial and Serumless Media

1938 • 7 citations

Chapter 9 Growth of Functional Glial Cells in a Serumless Medium

1975 • 5 citations

ARTIFICIAL FLUIDS AS UNINJURIOUS AS POSSIBLE TO ANIMAL TISSUES.

1896 • 4 citations